Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Single-domain antibodies"”.
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Generating single-domain antibodies against fibronectin splice variants
Here, I describe the process of generating single-domain antibodies which bind to splice variants of fibronectin containing EIIIA or EIIIB. Alpacas were immunized with either purified antigen cocktails, or from the ECM of tumor samples, and antibody libraries were generated. Using these libraries …
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Isolation and Characterization of Anti-SLP Single Domain Antibodies for the Therapy of C. difficile Infection
… characterized 11 SLP-specific recombinant single-domain antibodies (sdAbs), in terms of affinity and specificity, intrinsic stability, and ability to inhibit cell motility. Several sdAbs exhibit promising characteristics for a potential oral therapeutic based on their high affinity, high …
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Engineering functional recombinant proteins based on antibody domains and fragments of C. botulinum neurotoxin
… molecules. The focus of this study is single chain Fv (Fragment variable) and single domain antibodies. Recombinant botulinum neurotoxin fragments derived from serotype A, B, C and D are selected as backbones for an investigation into the feasibility and properties of novel recombinant …
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Application of silicon photonic microring resonators to the study of surface bioconjugation and protein capture agent binding kinetics
… immobilized hydrazines and aldehyde-modified antibodies is shown to be an efficient method to functionalize model oxide-coated sensor substrates with protein capture agents, allowing derivatization of substrates with reduced incubation time and sample consumption. The efficiency of this …
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Over-expression in Escherichia coli of the ToxA51 llama single domain antibody targeting Clostridium difficile enterotoxin A
Single domain antibodies (sdAb) have emerged as a family of novel antibodies that have vast applications. Of particular relevance, ToxA5.l, a sdAb capable of binding to, and therefore neutralizing, Clostridium difficile toxin A (TcdA) has been developed. The antibiotic resistant version of C. …
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Rational engineering strategies to improve antibody fragment pharmacokinetics and bioavailability
Therapeutic antibodies have become one of the most widely used classes of biotherapeutics due to their unique antigen specificity and their ability to be engineered against diverse disease targets. There is significant interest in utilizing truncated antibody fragments, including single-domain …
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Kinetics of Disordered Proteins and their Interactions
… as Aβ42 consists of using specially engineered single-domain antibodies (sdAbs) with a modified complementarity determining region (CDR). These complementarity determining regions (CDRs) are often disordered and their dynamics are poorly understood. I performed enhanced sampling simulations of …
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Advancing nanobody discovery and engineering with sequence-based machine learning
Nanobodies, also known as single-domain antibodies, are small antibody fragments naturally produced in camelids. Notable for their high expressibility, solubility, and stability, they exhibit binding affinities comparable to those of conventional antibodies. Since the approval of the first …
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Systematic development of rationally designed antibodies targeting predetermined epitopes of interest and protein aggregation
… of aggregation-prone regions of proteins using antibodies, but a major challenge in establishing antibodies against pre-selected epitopes is the effectiveness of the target regions to behave as antigens. To address this problem, a method of rational design was recently established in our group …
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Sélection et caractérisation de Nanobodies inhibiteurs de métallo-β-lactamases
… Ces fragments d’anticorps correspondent aux domaines variables des anticorps à chaîne lourde produits par les camélidés (HCAbs). Aussi appelés VHHs, ou « single-domain antibodies » (sdAbs), ils sont considérés comme les plus petits fragments fonctionnels dérivés d’anticorps, et peuvent être …
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Development of VHH- and antibody- based imaging and diagnostic tools
… a site for labeling in a controlled manner. Single-domain antibodies (VHHs) from camelids are amongst the smallest (15 KDa) proteins that can recognize a diverse set of targets with excellent specificity. Chemoenzymatic labeling of molecules using sortase allows site-specific attachment of a …
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B CELL EPITOPE MAPPING OF RIVAX, A CANDIDATE RICIN VACCINE ANTIGEN
… studies have shown that toxin neutralizing antibodies recognized four immunodominant regions on RTA i.e. four epitope clusters (namely cluster I to IV). RTA’s active site is surrounded by cluster III. Surprisingly, mAb IB2, which defines cluster III is the only antibody that recognized this …
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Targeting the N-myc oncoprotein using nanobody technology
… neuroblastoma. Nanobodies, camelid-derived single-domain antibodies, are a relatively new drug class. Whilst some are already in clinical trials for a wide range of diseases, these are specific for cell-surface or extracellular targets. However, their properties also make them ideal for use …