Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 103 for “"Single-cell analysis"”.
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Single cell analysis of Keap1-Nrf2 dynamics
… protein which allows Nrf2 to respond to changing cellular conditions. In the basal state, Nrf2 binds to two sites of a Keap1 dimer allowing its ubiquitination in a Cullin-3/Rbx1-dependent manner. In response to electrophiles and oxidants (termed inducers, which bind directly to Keap1) …
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Single Cell Analysis of the HIV-1 Latent Reservoir
… of rare, long-lived, latently infected cells. These cells, called the latent reservoir, are thought to resist immune clearance and viral cytopathic effects by harboring a transcriptionally quiescent integrated HIV-1 provirus. As a result, interruption of suppressive therapy almost …
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Single Cell Analysis of B Cell Responses to Malaria
… In particular, advances in technologies such as single cell RNA sequencing are allowing scientists to studying many of these aspects in ways never attempted before. It is known that the generation of a functional germinal centre (GC) to produce affinity matured long lived plasma cells (LLPCS) and …
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Single Cell Analysis of B Cell Responses to Malaria
… In particular, advances in technologies such as single cell RNA sequencing are allowing scientists to studying many of these aspects in ways never attempted before. It is known that the generation of a functional germinal centre (GC) to produce affinity matured long lived plasma cells (LLPCS) and …
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Massively multiplexed imaging probes for comprehensive single-cell analysis
… microscopy techniques are widely used to study cellular physiology in their native tissue environments. In particular, the use of fluorescent probes to tag different cell populations, subcellular compartments, specific proteins and nucleotide sequences has enabled examination of cellular …
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Development of High-Throughput Platforms for Single-Cell Analysis
… deal with highly heterogeneous populations of cells where the contributions of individual players cannot be ignored. Single-cell technologies aim to resolve this heterogeneity by analyzing many individual cells in a high-throughput manner. Here we developed two examples of such tools that rely …
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Automated hydrodynamic trap for single cell analysis in free solution
… to study inducible gene expression at the single cell level, albeit without the ability to control external stimuli with precise methods. Most are limited by long diffusive timescales to alternate environmental concentrations. In this work, we report a microfluidic-based platform for single …
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Integrated, single-cell analysis of transcriptional phenotype and clonotypic identity
Single-cell RNA sequencing (scRNA-seq) currently affords the ability to resolve whole transcriptomes of single cells with substantial throughput and has revolutionized studies of gene expression. Recent technical advances have enabled the matching of T cell receptor (TCR) and B cell receptor (BCR) …
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Single-cell Analysis of Structural and Immune Cell Interactions in Periodontitis
… provided insight into inflammatory pathways, single-cell RNA sequencing (scRNA-seq) enables detailed characterization of cell-type–specific responses and coordinated interactions within the periodontal microenvironment. OBJECTIVE: This study aimed to compare the transcriptional landscapes of …
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Development of enabling technologies for single cell analysis with mass spectrometry
… with robust performance. A growing field of single cell chemical analysis has arisen around these figures of merit. While early reports utilized manual isolation and extraction, recent developments in high-throughput sampling have enabled the examination of large populations of cells. One …
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High throughput microfluidic technologies for cell separation and single-cell analysis
The heterogeneity of individual cellular behavior in response to physical and chemical stimuli has raised increasing attention in many biological processes. There is great incentive in developing techniques for high throughput single-cell measurements and manipulations. Particularly, cell size has …
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Label-Free Microfluidic Devices for Single-Cell Analysis and Liquid Biopsies
… efficient, and have rapid processing and analysis. Microfluidic platforms in cancer research have proven to be advantageous due to its operation at the microscale, which has low costs, favorable physics, high precision, short experimentation time, and requires minimal reagent and sample …
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Single cell analysis of bacterial communication and gene transfer by Enterococcus faecalis
… resistance and transfer between E. faecalis cells is facilitated by cell-to-cell communication. This signaling triggers expression of genes from pCF10 that encode for transfer machinery. The response to signaling is robust and has been extensively studied at the population level. However, it …
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Single cell analysis by capillary electrophoresis with laser-induced native fluorescence detection
<p>Individual mammalian cells were analyzed by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). This technique was chosen due to its high separation efficiencies, small sample volumes and sensitive detection. Native fluorescence was used, in which the analyte was not …
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A genome-wide, single-cell analysis of vascular smooth muscle cell plasticity
Vascular smooth muscle cells (VSMCs) possess a remarkable capacity to change phenotype in response to injury or inflammation. In healthy arteries, VSMCs exist in a contractile state, but upon vascular inflammation or injury, they can switch into an activated state, in which they downregulate the …
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A Pan-Cancer Single-Cell Analysis of Intratumoral Copy Number Diversity and Evolution
… <p>To address these gaps, we developed Acoustic Cell Tagmentation (ACT), an advanced single-cell single-molecule DNA sequencing (scDNA-seq) technology, to resolve the copy number substructure of human tumors and investigate the evolution of aneuploidy. Applying ACT to eight triple-negative breast …
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Single-cell analysis of hematopoietic cell fate on niche-mimetic platforms using Raman microspectroscopy
… controlled and reproducible expansion of stem cells ex vivo. Hematopoietic stem cells (HSCs) are the best-studied class of tissue-restricted stem cells and their microenvironment in vivo has also been the subject of considerable research. Although a host of cellular, molecular and physical cues …
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Defining cell state regulators in cancers using single-cell analysis and CRISPR-Cas9 screening
… higher levels of plasticity. Development of single-cell profiling has had a particular impact for elucidating the composition of cell states occurring within individual tumours, and offers high-dimensional data which can be utilised for unsupervised signature extraction. The aim of this …
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Wavelength-Resolved Fluorescence Detection in Capillary Electrophoresis: Application to Single -Cell Analysis of Monoamine Neurotransmitters
The wavelength-resolved CE-LINF has found application to other small-volume mass-limited samples in complex matrices, such as clinical and environmental samples. The emerging advances in hardware, such as the availability of inexpensive and rugged deep ultraviolet metal vapor lasers, promise to …
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Efficient visualization for large-scale and high-dimensional single-cell data
… method for large-scale and high-dimensional single-cell data. Single-cell analysis can uncover the mysteries in the state of individual cells and enable us to construct new models of heterogeneous tissues. State-of-the-art technologies for single-cell analysis have been developed to measure …
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