Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 14 of 14 for “"Single-Nuclei RNA-Sequencing"”.
-
Molecular Mechanisms of Opioid Use Disorder in Human Brain Models
… angiogenic gene networks perturbed in the RNA and protein of OUD subjects, as well as downregulation of many neuron-correlated synaptic genes. Using single-nuclei RNA sequencing, I found global suppression of cell-cell signaling networks across all cell types, particularly involved in …
-
Towards dissecting neural ensembles : development of genetic profiling and targeting approaches
… ensembles. First, we present the development of single nuclei RNA sequencing methods and the application of the methods to chart the cellular diversity and its signature gene expression patterns in the adult mouse hippocampus. Second, we introduce rational design and predictable implementation of …
-
The Role of the Circadian Clock in the Paraventricular Nucleus of the Hypothalamus in Diurnal Energy Balance
<p>Circadian rhythms are internal biological rhythms that drive oscillations in physiology and metabolism. Maintained at the level of individual cells throughout the body, the circadian clock is necessary for driving tissue-specific temporal programs of energy balance. Though loss of the circadian …
-
Stromal interactions in pleural mesothelioma
… It is a stromal rich malignancy with cancer cell nuclei frequently accounting for less than 25% of all tumour nuclei. The remainder are largely comprised of stromal cells, the roles of which have been relatively neglected by existing studies. Of particular interest are the cancer-associated …
-
Epithelial cell plasticity in chronic liver disease (MASLD/NAFLD)
… is impaired due to disease burden, alternative regenerative mechanisms emerge to repair the liver parenchyma (Gadd et al., 2020). A potential mechanism of interest is the transdifferentiation of cholangiocytes into hepatocytes via either direct differentiation or via a progenitor cell …
-
The development and use of cutting edge next generation sequencing methodologies to study RNA viruses
With new next generation sequencing technologies and methodologies being published often, our ability to sequence viruses and host transcriptomes (in response to viral infection) has expanded – driving virology forward. Here we show the development and use of novel methodologies to study RNA …
-
Identifying unique cell states in early liver oncogenesis with transcription coupled repair
… appear to precede tumour initiation, as DNA sequencing has shown that cancer driver mutations can be found in otherwise-healthy tissues at high levels. Though an increase in mutational burden increases the risk of cancer, not all cells with driver mutations will form tumours. There is a …
-
Selective Neuronal Vulnerability in Neocortices from Patients with C9ORF72-related Neurodegeneration
… not yet been performed. With recent advances in single-cell and spatial transcriptomic approaches, our hypothesis can now be investigated in an unbiased fashion and at unprecedented resolution. The work in this thesis focuses on identifying selectively vulnerable neurons in the post-mortem …
-
Investigating iPSC-Derived Macrophage Stimulation with Oxidised Low-Density Lipoprotein as an Effective In Vitro Model of Foam Cells in Atherosclerosis
… potential foam cell-like populations using single cell RNA sequencing (scRNA-seq) of plaques, however due to known limitations of whole cell and more recent findings expanding our current knowledge of foam cell biology, much remains to be explored. Foam cells can be generated in vitro by …
-
Investigation into the Neurogenetics of Vocal Learning in Tursiops Truncatus, the Bottlenose Dolphin
… (LMC) and functionally analogous vocal learning nuclei in songbirds (HVC and RA). To screen for the putative “vocal learning” specialized subregion of the identified dolphin motor cortex, we conducted double-label Fluorescent in Situ Hybridization (FISH) experiments for SLIT1, a downregulated …
-
Quantitative spatial transcriptomics of the developing brain
… can be studied at the transcriptomic level using RNA sequencing methods, but techniques that quantitatively investigate the spatial dynamics of cell heterogeneity across large tissue areas at a single-cell level are lacking. Applying this capability to the developing brain would enable …
-
Myc and Cyclin T1 co-expression as a therapeutic strategy for cardiac repair following myocardial infarction
… mouse model setting and from a bicistronic mRNA vector could drive adult rodent cardiomyocyte cell-cycle re-entry in the context of injury and lead to functional improvement. Methods Whole-heart single-nuclei RNA sequencing (snRNAseq), in vivo experiments, histology methods, and molecular …
-
Elucidating intratumour heterogeneity through investigation of clonal, transcriptomic, and proteomic features across models of triple negative breast cancer and diffuse intrinsic pontine glioma
… challenges, this work utilises a combination of RNA sequencing based approaches, proteomics, and imaging techniques. The previously developed WILD-seq lineage tracing technology is applied to the D2A1-m2 model of breast cancer metastasis, enabling the tracing of barcoded clones through RNA …
-
Integrated Analysis of Molecular and Clinical Features in Melanoma Patients with Brain Metastases
… 1991 and 2015 at our institution with bulk RNA-seq data generated from their MBMs. On univariate analysis, the expression of multiple immune gene signatures was associated with improved OS. The gene expression signatures of several immune cell types positively correlated with OS, except for …