Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"Single-Molecule Detection"”.
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Unlocking single molecule detection sensitivities in surface-enhanced Raman spectroscopy
Single molecule detection sensitivities in surface-enhanced Raman spectroscopy require uniform fabrication of sub-5nm gaps between plasmonic nanoparticles over large areas. For this purpose, researchers have employed ultra-precise fabrication techniques like electron-beam lithography that offer …
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Sensing with Nanomaterials: Controlling Transport, Single Molecule Detection, and Voltage Sensing
Two-dimensional materials provide an intriguing means to not only study physical phenomena but also serve as disruptive membranes for ionic selectivity and sensing based applications. Atomic thinness of these materials affords a unique environment in an all-surface material to unlock challenges …
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Digital Protein Analysis: Technologies for Protein Diagnostics and Proteomics through Single-Molecule Detection
… including disease states. Protein biomarker detection is likely to play a major role in patient health and aid in the personalization of medicine. However, protein detection has lagged behind other bio-analytical methods due to intrinsic properties of proteins as well as the complexity of …
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Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis
Current clinical techniques for nucleic acid detection and analysis often involve PCR, lacking adequate specificity or sensitivity to meet the stringent requirements in certain applications. This research aims to develop an innovative molecular assay and the associated hardware to rapidly signal …
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Polymer-Based Microfluidic Devices for High Throughput Single Molecule Detection: Applications in Biological and Drug Discovery
… small footprints suitable for optical imaging. Single-molecule detection (SMD) is another technology which has emerged to facilitate the realization of high throughput data processing afforded by its ability to eliminate sample processing steps and generate results with high statistical …
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Genome analysis: mutation analysis using near infrared laser-induced fluorescence (NIR-LIF) and single molecule detection in microfluidic devices
… genetic analysis even in case where only a single copy of the DNA molecule is present in the sample. Research presented in this dissertation first examines the efficiency of in vitro amplification of single copy DNA with subsequent sequencing analysis of PCR product. The PCR products …
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Engineering Noble-Metal Nanoparticles for Sensing and Imaging with Surface-Enhanced Raman Scattering
… mechanism and to implement this technique for single-molecule detection and imaging. In addition, SERS was used as a sensitive probe to study molecules confined to a nanoparticle's surface. The first part of this work focused on measuring the SERS from different Ag and Au nanoparticles and …
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Exploring transport and phase behavior in nanoporous carbon materials
… on applications involving membrane fabrication, single-molecule detection, oil reservoir modeling, and drug delivery. While transport and phase behavior in larger nanopores (>50 nm) approach bulk or are well-described by continuum models, much less is known about much smaller nanopores where the …
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Nanopores for detecting and sensing biological molecules
In spite of significant advances in the detection, separation and counting of single biological molecules (DNA, proteins, aminoacids, etc.) with solid-state nanopores, atomically-resolved scanning and detection of these molecules remains a significant challenge. In most nanopore-based DNA …
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Analysis of Polyethylene Glycol in the α-Hemolysin Nanopore
… been shown to be a useful analytical tool for single molecule detection. They have been used to study the composition of DNA and other molecules of interest. These pores are usually α-hemolysin which is a toxin from Staphylococcus aureus or more recently nanoscale synthetic solid state pores. …
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Scanning tunneling microscopy and spectroscopy of wet chemically synthesized porous graphene nanoribbons on hydrogen passivated silicon (100)
… expand the possibilities for applications in single-molecule detection and selectivity, including desalination and DNA sequencing.
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Advanced nucleic acid detection with solid-state nanopores: from fundamental understanding to cutting-edge platforms
… sensing has emerged as a powerful technique for single molecule detection, due to its operational simplicity, high sensitivity, and real-time analytical capability. Despite these advantages, significant challenges remain in achieving multiplexed detection of diverse targets and in enabling …
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Development of the single-molecule tracking and fluorescence lifetime techniques for biophysical measurements and biomedical applications
Single-molecule detection allows us to reveal the biophysical features of rare species in complex samples. Direct characterization of annealing/melting kinetics of nucleic acids without the need for synchronization of molecular states can be achieved with single-molecule detection, but the current …
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Single-molecule orientation estimation using a polarisation camera
… for measuring the orientation and position of single fluorescent molecules. The method - named POLCAM - is based on the use of a polarisation camera. Current methods for single-molecule orientation estimation require optical setups and algorithms that can be prohibitively slow and complex, …
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Protein and RNA folding: from bulk towards high throughput single molecule experiments
… of a high throughput time correlated single photon counting experiment is presented. Drops of ~10 μm diameter are optically trapped and interrogated by a femtosecond probe laser beam. Fluorescence photons emitted by the sample inside the drop are collected in all 4π steradians with …
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Fabrication of Hollow Optical Waveguides on Planar Substrates
… in liquid-core ARROW waveguides have achieved single-molecule detection sensitivity. Integrated optical filters based on ARROW waveguides were fabricated, and preliminary results of a capillary electrophoresis separation device using a hollow ARROW indicate the feasibility of such devices for …
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Development of Original and Notable Techniques for Protein Analysis, Native Identification, and Characterisation
… sizing, combined with confocal microscopy. The single molecule detection allows to distinguish particles in function of their brightness, breaking the homogenous limitation of these microfluidic techniques under classical epifluorescence microscopy. The fourth chapter shows an elegantly simple …
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Studying Models of the Amyloid Cascade Hypothesis Using Single-Molecule Imaging
… Aβ aggregates. Our group has been developing single-molecule and super- resolution microscopy techniques to characterise these aggregates. While these methods were previously applied to human samples, they have not been used to study disease models. Cellular and animal models have numerous …
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Polyelectrolyte multilayers (PEM) in micro / nanofluidics for novel BioMEMS platforms
… channels offer new opportunities for advanced biomolecule manipulation and separation science because they provide unique capabilities such as ion-perm selectivity and nanometer-sized structures. In order to establish industrial applications for biotechnology and medicine, including separation of …
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Resonant Detection of Nano to Microscopic Objects Using Whispering Gallery Modes
… medium. This thesis demonstrates how label-free molecules binding to the microsphere surface perturb the optical resonance by interaction with this evanescent field. The effect is demonstrated by surface adsorption of a protein (serum albumin). The general use as a biosensor is shown by specific …
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