Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Single molecule fluorescence resonance energy transfer (smFRET)"”.
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The Role of Initiation Factor Dynamics in Translation Initiation
… in Escherichia coli. Specifically, I have used single-molecule fluorescence resonance energy transfer (smFRET) as a primary tool for investigating how the dynamics of IF1 and IF3 regulate the accuracy with which the translational machinery selects an initiator transfer RNA (tRNA) and the correct …
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Single-molecule FRET analysis of the dynamics of AMPA receptor
Single-molecule fluorescence resonance energy transfer (smFRET) spectroscopy has advanced to become a powerful tool in characterizing biomolecules to the molecular level. In this thesis, the smFRET technique was utilized for the first time to analyze the dynamics of the conformational changes of …
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The Molecular Mechanism of the Escherichia Coli vitamin B12 Transporter BtuCD-F: Real-time Observation of the Transporter in Motion
… BtuCD-F reconstituted into lipid bilayers using single molecule fluorescence resonance energy transfer (smFRET) measurements. Fluorescent probes have been introduced at a variety of diagnostic sites, enabling smFRET to be used to measure distance changes in different conformational states as well …
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Single-molecule fluorescence resonance energy transfer study of SNARE-mediated membrane fusion
… of protein-mediated membrane fusion through single-molecule fluorescence resonance energy transfer (smFRET). Membrane fusion is one of the important cellular processes by which two initially distinct lipid bilayers merge their hydrophobic cores, resulting in one interconnected structure. For …
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Study Of Protein-Rna Interactions Using Fluorescence Resonance Energy Transfer (fret) And Single-Molecule Fret
… and dynamics of RNPs are poorly understood. Single-molecule Fluorescence Resonance Energy Transfer (smFRET) techniques that provide information about heterogeneity and dynamic behaviors of molecules have been developed to investigate inter- and intra-molecular interactions. Here we have used …
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Characterization of leucyl-TRNA synthetase from homo sapiens and escherichia coli in aminoacylation, amino acid editing and interdomain interactions
… the connective polypeptide 1 domain (CP1). Post-transfer editing involves the translocation of mischarged tRNA from the aminoacylation to the editing active site where mischarged tRNA binds for hydrolysis of the noncognate amino acid to enhance fidelity. Based on crystal structure analysis, the …
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Single molecule studies of helicase mechanisms
… resolutions, as well as the ability to look at molecules one at a time instead of in an ensemble, have been shown to be helpful in elucidating the specific actions in many biological systems. Single molecule FRET (smFRET) microscopy provides these abilities with a distance scale of 2-8 nm, 8 ms …