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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 99 for “"Single molecule fluorescence"”.
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Single molecule fluorescence studies of replicative helicases
… into mechanical energy for translocation on single stranded (ss) DNA and unwinding of double stranded (ds) DNA. All of the 12 known replicative helicases are hexameric helicases that are ring-shaped in structure and are homohexamers with the exception of the eukaryotic minichromosomal …
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Single molecule fluorescence studies of biomolecular interactions
Single molecule fluorescent techniques have become standard approaches to study protein-DNA interactions. However, these techniques have largely been confined by limitations in assays to studying the interaction between simple DNA substrates and a single protein. During my PhD I developed several …
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Biophysical Applications of Single Molecule Fluorescence Localization
Fluorescence single molecule localization has become a powerful tool for investigating problems in biological physics. However, there are numerous limitations on the types of measurements that can be made with typical instrument designs based on objective-type TIR and wide field CCD imaging. We …
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Single molecule fluorescence microscopy of carbon nanotubes
Single molecule microscopy has been extensively used in the past decade to study individual biomolecules with excellent spatial and temporal resolution. Meanwhile, characterization of nanomaterials and their development towards a variety of biological applications has progressed rapidly. Despite …
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Characterization of splicing mechanisms by single-molecule fluorescence
… cofactor, the DEAD-box protein Mss116, using single-molecule fluorescence. Consistent with folding studies at very high magnesium concentrations, our single-molecule data show that Mss116 can promote the folding of group II introns under near physiological conditions <i>in vitro</i>. …
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Single-Molecule Fluorescence Studies of ReAsH and Myosin VI
Single-molecule fluorescence spectroscopy has become one of the most powerful techniques in chemistry and biology. Recently, a new fluorescence technique called fluorescence imaging with one nanometer accuracy (FIONA) was shown to localize the position of a fluorescent molecule within a nanometer. …
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Single molecule fluorescence and force spectroscopy of hairpin ribozyme
Submitted by Elizabeth Kent (eckent2@illinois.edu) on 2012-06-07T18:47:11Z No. of bitstreams: 1 2006_nahas.pdf: 5361832 bytes, checksum: 0d81d12a930597d8c5ce651d401a9bd7 (MD5)
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Single molecule fluorescence spectroscopy of ClpXP-mediated substrate degradation
… cleavage occurs. Here, we present the single molecule fluorescence assay for probing the interaction between the ClpXP enzyme and its substrates. A covalently crosslinked ClpX hexamer maintain functionally stable form at the low concentration of single molecule level. Surface …
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Single Molecule Fluorescence Studies of Prions and Prion-like Proteins
… and Parkinson’s disease. In this thesis, I use single-aggregate imaging to study fibril fragmentation and elongation of individual murine PrP aggregates from seeded aggregation in vitro. From fluorescence imaging of individual PrP aggregates on the coverslip surface, elongation and fragmentation …
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Exploring the mechanome with optical tweezers and single molecule fluorescence
The combination of optical tweezers and single molecule fluorescence into an instrument capable of making combined, coincident measurements adds an observable dimension that allows for the examination of the localized effects of applied forces on biological systems. This technological advance had …
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Catalytic Studies Of Noble Metal Nanocatalysts Using Single-Molecule Fluorescence Microscopy
… heterogeneous, which makes studying them at the single-particle level desirable, as it then becomes possible to quantify these differences. A highthroughput fluorescence microscopy technique was developed in which a nonfluorescent reactant gets catalyzed to a fluorescent product on a gold …
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Single-molecule fluorescence resonance energy transfer study of SNARE-mediated membrane fusion
… of protein-mediated membrane fusion through single-molecule fluorescence resonance energy transfer (smFRET). Membrane fusion is one of the important cellular processes by which two initially distinct lipid bilayers merge their hydrophobic cores, resulting in one interconnected structure. For …
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Structural Organization and Chemical Activity Revealed by New Developments in Single-Molecule Fluorescence and Orientation Imaging
<p>Single-molecule (SM) fluorescence and its localization are important and versatile tools for understanding and quantifying dynamical nanoscale behavior of nanoparticles and biological systems. By actively controlling the concentration of fluorescent molecules and precisely localizing individual …
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Using optical tweezers, single molecule fluorescence and the ZIF268 protein-DNA system to probe mechanotransduction mechanisms
… chemistry, such a bead can be attached to a single molecule as a handle, permitting the application of force on the single molecule. Measuring the force applied in real-time is dependent on detecting the bead's displacement from the trapping laser beam axis. Back-focal-plane detection …
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Combined optical trapping and single molecule fluorescence to study the force-dependent binding kinetics between filamentous actin and its partners
… binding kinetics of such proteins at the single molecule level by using an instrument that combines optical trapping with single molecule fluorescence. The main focus of this work was the design and construction of the experimental equipment. The results show position detection …
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Theoretical study of single-molecule spectroscopy and vibrational spectroscopy in condensed phases
… are employed to study several problems of single-molecule spectroscopy and vibrational spectroscopy in condensed phases. The first part of the thesis concentrates on studying dynamic disorders probed by single molecule fluorescence spectroscopy. Event statistics and correlations of …
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Fluorescent Tools for Watching Conformational Changes of Biological Molecules
Finally, we used a single molecule fluorescence quenching assay to study the timing of the neck linker conformational change that occurs as kinesin walks processively along a microtubule. We observed repetitive, alternating cycles of fluorescence and quenching as individual kinesin motors walked …
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Fluorescence imaging microscopy studies on single molecule diffusion and photophysical dynamics
Within the last years, e.g. by investigating the fluorescence of single molecules in biological cells, remarkable progress has been made in cell biology extending conventional ensemble techniques concerning temporal / spatial resolution and the detection of particle subpopulations [82]. In addition …
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