Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Single chain variable fragments"”.
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Synthesis and biological evaluation of auristatin-F recombinant antibody-drug conjugates for cancer therapy
… DNA technology, and incorporated antibody single-chain variable fragments (scFvs) exhibiting specificity towards the epidermal growth factor receptor (EGFR), chondroitin sulfate proteoglycan receptor (CSPG4) and CD44 receptors more highly expressed in various cancer types. The design and …
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Genetic engineering of recombinant anti-mycolic acid antibody fragments for use in tuberculosis diagnostics
Mycolic acids are long chain lipids from the cell walls of Mycobacterium tuberculosis. The Nkuku phage display library was previously used to obtain monoclonal antibody binders to mycolic acids. In total 11 binders were obtained of which one was selected (MAC10) for further investigation by genetic …
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Developing novel SNAP tag-based antibody fusion proteins directionally conjugated to fluorophores for immunophenotyping of acute myeloid leukemia
… next-generation sequencing, real-time polymerase chain reaction and cytogenetic analysis. Immunophenotyping by flow cytometry and immunohistochemistry can be used to identify specific proteins or antigens upregulated in AML. In low and middle-income countries, particularly Africa, AML diagnosis is …
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Engineering of recombatinant antimalria antibodies for application in paratransgenesis
… for bacterial expression and formatted as single chain variable fragments (scFv). Synthetic genes encoding the scFv 4B7 and Pf-NPNA-1 were constructed, with varying linker length, in the VH-VL and VL-VH orientation. The scFvs were cloned into different expression plasmids to evaluate a …
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Golgi Specificity and Development of Autoreactive B Cells
<p>B cell receptors with certain heavy/light chain pairs predominate in the periphery of anti-DNA heavy chain transgenic mice while certain other B cell receptors are absent. We wanted to know why. To answer this, we re-constructed B cell receptors represented in the anti-DNA transgenic mice by …
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Evaluation of tumour-associated antigens to optically label cutaneous basal cell carcinoma for surgical excision
… superficial and deep layers of the skin by a single-excision procedure. We used an integrated computational tool to re-analyze publicly available cDNA microarray data in combination with theoretical search to identify BCC-associated antigens. Accordingly, six different antigens were selected …
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Generation and application of recombinant antibody fragments for prostate cancer detection.
… of murine and avian recombinant antibody fragments to free PSA (fPSA) was performed. Animals were immunised, RNA extracted and cDNA synthesised. The antibody variable genes were amplified by PCR, gel-purified and cloned into a phagemid vector. Electrocompetent cells were transformed and …