Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 169 for “"Single cell level"”.
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Quantitative investigation of bacterial chemotaxis at the single-cell level
Living cells sense and respond to constantly changing environmental conditions. Depending on the type of stimuli, the cell may response by altering gene expression pattern, secreting molecules, or migrating to a different environment. Directed movement of cells in response to chemical stimuli is …
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Regulation of the Anaphase-Promoting Complex Examined at the Single Cell Level
<p>Cell cycle transitions are driven by oscillations of cyclin-cyclin dependent kinase (CDK) activity and associated cyclin degradation, mediated by ubiquitylation by the anaphase-promoting complex (APC). In this work, I analyzed the regulation of the APC by its activator Cdh1 in budding yeast in …
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Quantification of microRNA regulation and its consequences at the single cell level
… have been found to participate in almost every cellular process and their dysregulation is associated with many diseases. miRNAs recognize their targets by base paring to miRNA response elements (MREs), which are predominantly located at 3' untranslated region (3'UTR) of mRNAs. This thesis …
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A toolset for linking phenotype and gene expression at the single-cell level
The development of single-cell RNA-sequencing has led to a new degree of resolution in the characterization of complex, heterogeneous biological systems. However, existing methods are often limited in their ability to link these whole-transcriptome profiles with complimentary measurements of …
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Gene expression profiling in different stages of development of Arabidopsis thaliana leaftrichomes at the single cell level
Each organ of a multicellular organism is unique at the level of its tissues and cells. Furthermore, responses to environmental stimuli or developmental signals occur differentially at the single cell or tissue level. This underlines the necessity of precise investigation of the “building block of …
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A microfluidic approach for the initiation and investigation of surface-mediated signal transduction processes on a single-cell level
… transduction processes that are induced by cellular interactions, defined events along the signal transduction cascade and subsequent activation steps have to be analyzed and then also correlated with each other. This cannot be achieved by ensemble measurements because averaging biological …
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BIOINFORMATICS ANALYSIS OF FULL-LENGTH RNA NANOPORE SEQUENCING AT SINGLE CELL LEVEL TO STUDY TRANSCRIPTIONAL AND MUTATIONAL PATTERNS IN HUMAN LEUKEMIA
… cancer originating from hematopoietic stem cells, exhibits substantial intra-tumoral heterogeneity impacting disease development, prognosis, and treatment response. However, understanding the interplay between various layers of heterogeneity at the single-cell level remains limited due to …
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Controlling Microbial Adhesion to the Surfaces Using Topographical Cues
… nanofiber-textured model surfaces is analyzed at single-cell level. The results reveal similarities between the effect of topography on bacteria-surface interactions and vesicle-surface interactions. The results are discussed in the context of controlling bacterial adhesion to surfaces using …
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INTEGRATED SINGLE-CELL MUTATION, GENE EXPRESSION AND ISOFORM ANALYSIS TO DECONVOLVE ACUTE MYELOID LEUKEMIA HETEROGENEITY
… cancer arising from the hematopoietic stem cell (HSC). As other tumor types, AMLs are characterized by multiple and interconnected levels of intra-tumor heterogeneity, including genetic (DNA mutations), phenotypic (transcriptional patterns) and ecological (interactions with host …
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Visualizing Allele Specific Expression In Single Cells
Single molecule RNA FISH techniques have enabled the quantification of gene expression at the single cell level, revealing significant variability that has been previously obscured by techniques measuring population averages. These techniques, however, have been limited in that they cannot classify …
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Computational Modeling of Bacterial Biofilms
… measurements of complex bacterial communities at single-cell resolution are now possible to obtain. Guided by these rich experimental data sets, we improve a recently proposed three-dimensional individual-based simulation framework to uncover governing microscopic dynamics at single-cell level …
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Cellular responses to mechanical stresses applied via magnetic manipulators
A mechanotransduction model aims to explain how a cell senses mechanical forces and translates them into molecular events, such as changes in intracellular ion concentrations, gene expression, post-translational protein modification, or cytoskeletal redistribution. Many studies of …
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Development and Application of a Microfluidic Cultivation Platform for the Analysis and Control of Industrially Relevant Synthetic Microbial Co-Cultures
… now, microfluidics has been mainly used for single-cell analysis of monocultures.<br />In this thesis, different chip designs and model consortia were developed to analyze and control the interaction of synthetic co-cultures. In a first study, it was shown that unilateral dependence can be …
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The role of criticality of gene regulatory networks on emergent properties of biological systems
… networks (GRNs) and dynamics of GRNs at a single cell level has been vigorously studied. However, the relationship between the criticality of GRNs and properties of multicellular organisms at a higher level has not been fully explored. Here we aim at revealing potential roles of the …
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Causal Representation Learning for Predicting Genetic Perturbation Effects on Single Cells
… deepened our understanding of gene regulation in cells. Among these, Perturb-seq has emerged as a powerful technique, enabling high-resolution profiling of transcriptomic responses to genetic perturbations at the single-cell level. Such insights have profound implications for functional genomics …
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Methods to interrogate cells and their interactions with single-cell resolution
… and unbiased representations of diverse intracellular molecules at the single-cell level. With this technological advancement, researchers have begun deconvolving population-level measurements to understand whether prior observations were homo- or heterogeneous across the sample. In order to …
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Modeling of gene regulatory networks controlling cell-fate decisions in Bacillus subtilis
To adapt to various environments, bacterial cells can activate distinct gene-expression programs and differentiate into mutually exclusive cell types. This process is called cell-fate decision. Bacillus subtilis is a well-studied model system for investigating bacterial cell-fate decisions. In …
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Observing microbial processes at the microscale with In Situ technology
… where nutrient concentrations exceed background levels by orders of magnitude. While the impact of this microscale heterogeneity has been investigated in the laboratory with microbial isolates and theoretical models, microbial ecologists have lacked adequate tools to interrogate microscale …
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