Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"Single cell genomics"”.
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Decoding Copy Number Substructure and Evolution From Single Cell Genomics
… However, these observations rely on limited cell numbers and inherent experimental bias from first-generation single cell technologies. Therefore, the evolutionary trajectory after the punctuated burst remains unknown. To address this question, we sequenced 9,765 cells from 8 primary TNBCs …
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Discovering variation from cell atlases: comparative methods for single-cell genomics
Single-cell genomics technologies have become the norm to investigate cell-to-cell heterogeneity and collective research efforts have built “cell atlases” to characterize previously unknown cell types and states in tissues. For this task, the collection of cells from multiple donors or tissue sites …
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Single-cell genomics in rabbit and mouse elucidate eutherian embryonic development
… accessibility profiles of almost 180,000 single cells and high-resolution histology sections from embryos spanning gastrulation, implantation, amniogenesis, and early organogenesis. Using a novel computational pipeline, we compare the transcriptional landscape of rabbit and mouse at the …
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Investigating The Ecology and Evolution of Normal Breast Tissues and Breast Cancer With Single Cell Genomics
<p>There is vast cellular heterogeneity in human breast tissues, with different transcriptional programs in the stromal, epithelial, and immune components, however, it remains unclear how their reprogramming and interplay leads to the progression of invasive phenotypes such as Triple- Negative …
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Application of the single cell genomics in deciphering tumor heterogeneity and its role in tumor progression and drug resistance
Tumor progression, from the single mutated cell to the advanced stages of cancer, represents an evolutionary process. During tumor progression, cancer cells acquire new genetic mutations, becoming more heterogeneous, leading to tumor progression and resistance to therapy. However, clear genetic …
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Investigating The Rare Aneuploid Cells in Normal Breast and Therapeutic Resistance in Triple Negative Breast Cancer Using Single Cell Genomics
<p>Aneuploid epithelial cells are common in breast cancer, however their presence in normal breast tissues is not well understood. To address this question, we applied single cell DNA sequencing to profile copy number alterations (CNAs) in 83,206 epithelial cells from breast tissues of 49 healthy …
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Profiling Host Respiratory Responses to SARS-CoV-2 Infection
Single-cell genomics have revolutionized the granularity with which we can dissect cellular phenotypes in both health and disease. Global atlases of homeostatic tissues have revealed rare cell populations critical to organ function and provide comprehensive genomic reference maps for the scientific …
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Cells of the Developing Human Heart and Great Vessels
… heart and the great vessels comprise various cell types that collectively support the heart’s homeostasis. Single-cell genomics has expanded our knowledge of the heart’s cellular heterogeneity. By gaining a deeper understanding of its cellular makeup, we can uncover how these cells converge to …
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Tissue-resident immunity in the human kidney
… are subserved by a wide array of specialised cell types with distinct developmental origins and anatomical organisation. Amongst these cells lies a network of tissue-resident immune cells with incompletely characterised roles in tissue homeostasis, repair, and defence. Furthermore, different …
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Computational methods for dissecting multicellular mechanisms of complex diseases
Single-cell genomics technologies have enabled unbiased characterization of cell types and cellular states. However, the high-dimensional nature of this data necessitates computational and statistical methods to uncover the biological processes that shape it. In my thesis research, I developed …
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Multilineage modelling of early placental responses to pathogens at single-cell resolution
… placental models such as human trophoblast stem cells (hTSCs), organoids, and placental explants, and perform a comprehensive single-cell transcriptomic analysis of their responses. Firstly, I outline the physiological structure and role of the placenta in supporting and protecting the developing …
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Improving genome assembly by identifying reliable sequencing data
… (WGA) data is more challenging compared to multi-cell data due to the coverage variation introduced by the amplification step (MDA, MALBEC, etc.), which implies that applying a simple k- mer frequency cutoff is unreasonable. We observed that with sufficient coverage, using partial reads (read …
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Dissecting cellular crosstalk and genetic perturbations in haematopoietic regulation
Haematopoietic stem and progenitor cells (HSPCs) proliferate and differentiate to produce a large number of mature blood and immune cells on a daily basis while maintaining themselves as the reservoir for lifelong haematopoiesis. Haematopoiesis is dynamically regulated by activating …
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Single cell -omic landscapes in normal and perturbed haematopoiesis
The use of single cell genomics technologies to generate atlases of healthy tissues has served both as a valuable comparator resource for perturbed datasets, and to permit the discovery of novel biological processes in the differentiation of stem cells into mature tissues. Large scale profiling of …
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Understanding Development of Human Immunity One Cell at a Time
The emergence of single-cell and spatial multi-omic technologies has revolutionized our understanding of the immune cells. The international Human Cell Atlas consortium has spearheaded and coordinated a global effort to construct atlases of human tissues across multiple developmental stages. This …
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Deciphering Features of Protective or Maladaptive Cellular Immunity in the Airways Following Primary and Repeated Pathogen Exposure
… from homeostatic conditions. The airway’s cellular constituents – epithelial, stromal, and immune cells – maintain local and global homeostasis by facilitating gas exchange and providing a barrier against noxious environmental agents (e.g., xenobiotics, allergens, toxins, and microbes). …
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Comparative single-cell transcriptomics of complete insect nervous systems
The brain is made of many types of specialized cells. Information processing in these cells is responsible for generating innate and learnt behaviour. Gene expression, neuron activity, and neuron connectivity influence information processing, but bridging the gap between genes, circuits and …
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Using single-cell RNA-seq to assess the effect of common genetic variants on gene expression during development
… opportunity to measure gene expression at the single cell level, and the chance to study cellular heterogeneity. This represents a remarkable advance over traditional bulk sequencing methods, particularly to study cell fate commitment events in development. The challenge of studying early human …
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Systematic analysis of the molecular mechanisms of T cell entry into tissues
It has now become evident that a subset of T cells establish residency in non-lymphoid tissues and function to provide local immune protection and tissue homeostasis. Classically, immunologists have inferred immune function from immune cells sampled from the blood and lymphoid organs, despite the …
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