Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Single Turnover"”.
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Single-Enzyme Kinetics with Fluorogenic Substrates: Strategies Towards Accurate Single-Turnover Detection
Contains fulltext : 161454.pdf (Publisher’s version ) (Open Access)
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Thermodynamic and kinetic regulation of coupling factor
… was assessed by monitoring the number of single-turnover flashes needed to initiate flash-induced ADP phosphorylation at varying $\Delta$G$\sb{\rm ATP}$ values.
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Studies on the Efficiency of Energy Conservation in the Initial Stages of Photophosphorylation
… so incubated were illuminated by a train of single turnover flashes, ATP synthesis began long before the added Tricine could have been titrated.
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Metal complexes of non-heme ligands: biological applications
… and chymotrypsin were inactivated by ferryls (single turnover), and by an iron complex in presence of biologically relevant oxidant, H<sub>2</sub>O<sub>2</sub> (catalytic conditions). Analysis of the CA–I inactivation products by SDS–PAGE, ESI–MS and LC/MS/MS confirmed that the protein is …
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Synthesis Of N-Acetyl And C-Tert-Butyl Natural Amino Acid Substrates That Mimic Residues Of Polypeptides And Their Reactivity With [feiv(o)(n4py)]2+: Kinetic And Mechanistic Investigations
… of glutathione by metal-based oxidants, both single turnover and catalytic reactions. The introduction discusses the importance of glutathione in biology and the mechanism of its oxidation by copper ions. The discussion sections cover two related projects. The first is the single turnover …
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Seasonal variability of phytoplankton photophysiology in the Southern Ocean: an analysis of uncertainties and the impact of assumptions
… phytoplankton productivity is through active single-turnover chlorophyll-a fluorescence (ST-ChlF) techniques, such as Fast Repetition Rate fluorometry (FRRf), which infer photosynthetic capacity by measuring the transport of electrons during photosynthesis. While promising, this technique …
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Comparison of two measures of PSI electron transport in winter rye
… change at 820nm . It was found, with a single turnover flash, that thylakoids isolated from cold grown plants have a 50% greater number of P700 oxidized than thylakoids isolated from warm grown plants. Incubation of thylakoids in the dark at 35 C did not change the number of P700 …
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Molecular engineering of new protein labeling methodology based on rational design and in vitro evolution
… 25 was obtained in a model selection. Due to the single-turnover nature of the selection, however, this scheme was not able to enrich highly active catalysts over moderately active ones. In separate work, we turned our attention to the streptavidin-biotin pair. Again using bead-less IVC, we …
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The reaction kinetics and three-dimensional architecture of a catalytic RNA
… its ability to promote a reaction similar to a single step in RNA polymerization: attack of a primer 3'-hydroxyl on a 5'-triphosphate, with formation of a new 3'-5' bond and release of pyrophosphate. Derivatives have been shown to catalyze general primer extension reactions, making the ligase a …
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Heterogeneous reservoirs in the marine carbon cycle
… are then derived for the specific case of a single turnover time and a lognormal distribution. We apply these ideas to the problem of dissolved organic carbon (DOC) cycling in the ocean. Current models of bulk concentration and isotope data suggest a microbially sourced DOC reservoir …
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Lesion Bypass of N2-ethylguanine by the Human Y Family DNA Polymerases Iota and Kappa
… were determined using steady-state and single turnover kinetic assays with Mg2+ or Mn2+ as the activating metal. The efficiency of dCMP incorporation opposite N2-ethylGua by the DNA pol ι is increased ~2000-fold in the presence of Mn2+ compared to Mg2+. The increased efficiency is due to …
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Characterisation of phenol hydroxylase and its auxiliary proteins, DmpM and DmpK, from pseudomonas spl Strain CF600
… up of three copurifying polypeptides, DmpLNO. Single turnover experiments confirm that DmpLNO contains the active site, but requires DmpM for efficient turnover. Mössbauer, EPR and UV/vis spectroscopy data, demonstrated the presence of two types of binuclear iron centers in DmpLNO. Treatment …
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The structural basis of RNA-catalyzed RNA polymerization
… catalyzes a reaction chemically identical to a single turnover of RNA-dependent RNA polymerization. Such an activity would have been requisite for the emergence of a self-replicase ribozyme, an enzyme that, according to the RNA World hypothesis, would be fundamental for the emergence of life. …
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Heterogeneous reservoirs in the marine carbon cycle
… are then derived for the specific case of a single turnover time and a lognormal distribution. We apply these ideas to the problem of dissolved organic carbon (DOC) cycling in the ocean. Current models of bulk concentration and isotope data suggest a microbially sourced DOC reservoir …
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Exploring the reactivity of bacterial multicomponent monooxygenases
… in the presence of the regulatory protein PHM. A single longlived diiron(III) intermediate with 6 = 0.59 mm/s and A EQ = 0.63 mm/s and no discernable optical bands accumulates along the reaction pathway. The spectroscopic parameters of this intermediate are similar to those reported recently for a …
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Enzymology of DHHC-mediated Protein S-Acylation
… for classic steady-state kinetic experiments. Single turnover assays supported the hypothesis with radiolabeled fatty acid transferring from acyl-DHHC to protein substrate. Unexpectedly, these investigations also revealed that DHHC proteins display different acyl-CoA chain length preferences. A …
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Catalytic Mechanism and Maturation of the Metalloenzyme Nitrile Hydratase
<p>Nitrile hydratases are metalloenzymes that catalyze the hydration of nitriles to their corresponding amides in a specific manner at ambient pressures and temperatures at neutral pH. Traditional industrial methods require high temperature and pressure, extreme pH, and heavy metals. NHases are …
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Understanding orchestrated chemical reactions in toluene/o-xylene monooxygenase from pseudomonas sporium OX1
… Steady-state activity, regiospecificity, and single-turnover yields were also determined for the T201 mutants. Dioxygen activation by the reduced T201 variants was monitored by stopped-flow UV-vis and M6ssbauer spectroscopy. These studies demonstrated that the same dioxygen activation …
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Exploring reactivity and component interactions in Toluene/o-Xylene Monooxygenase from pseudomonas sp. OX1
… Through characterization of the steady-state turnover of WT ToMOH and three mutant enzymes, N202A, Q228A, and Q228E, we demonstrate that these residues are critical for turnover. Mechanistic analysis reveals that these residues are critical for water egress and efficiently consuming NADH to …