Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 366 for “"Single Stranded"”.
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Molecular Dynamics Study of Single Stranded Peptide Nucleic Acids
… the conformational and dynamical properties of single stranded <em>aeg</em>PNA and <em>cp</em>PNA were undertaken to determine how the cyclopentane ring will improve binding and to determine the contributions of both entropy and dihedral angle preference to the observed stronger binding. The …
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Transforming Activity in Single-Stranded Dna From Bacillus Subtilis
Made available in DSpace on 2014-12-09T17:35:44Z (GMT). No. of bitstreams: 1 6711832.pdf: 3258275 bytes, checksum: df05fff3da0ff175a81f77aff9db44d9 (MD5) Previous issue date: 1967
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Growth and deterministic assembly of single stranded carbon nanotube
… This method includes vertical growth of single strand carbon nanotubes, pellet casting, planarization, pellet separation, transplanting and bonding. A new CNT PECVD machine has been designed and built to custom fit to our specifications for vertically grown single strand CNTs.
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Single-Stranded Dna Scanning And Deamination With Single-Molecule Resolution
<p>SINGLE-STRANDED DNA SCANNING AND DEAMINATION WITH SINGLE-MOLECULE RESOLUTION</p> <p>by</p> <p>GAYAN SENAVIRATHNE</p> <p>December 2013</p> <p>Advisor: Dr. David Rueda & Dr.Ashok Bhagwat</p> <p>Major: Chemistry (Analytical) </p> <p>Degree: Doctor of Philosophy</p> <p>APOBEC3G (Apo3G) and …
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Graphitic Surface Attachment by Single-Stranded DNA and Metal Nanoparticles
… to be explored. The noncovalent binding of single-stranded DNA oligonucleotides to carbon nanotubes and graphene has been seen to give rise to effective gas sensors. We examine similar systems to each of these in turn, imaging carbon nanotubes decorated with single-stranded DNA in …
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Fluorescence imaging of single molecule dynamics on long single stranded DNA
Recent advances in single-molecule fluorescence imaging techniques have allowed the direct observation of protein dynamics on DNA, but the progress has been largely limited to double-stranded DNA (dsDNA) or short single-stranded DNA (ssDNA). Here, a single molecule imaging approach for observing …
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Immunostimulatory and Cellular Toxic Effects of Single-Stranded RNA On Cancer Cells
<p>Whether 5'-triphosphate-single-stranded-RNA (5 '-ppp-ssRNA) is immunostimulatory was controversial in the literature. In order to clarify the immunostimulatory effect of 5'-ppp-ssRNA, we synthesized ssRNAs and tested the IFNp (interferon-beta) level and cellular toxicity of ssRNAs in SKOV3 …
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Comparative Immunological Analyses of Anti-Deoxynucleotide and Anti Single-Stranded Dna Antibodies
Made available in DSpace on 2014-12-14T07:14:48Z (GMT). No. of bitstreams: 1 8004137.pdf: 6213863 bytes, checksum: d5b92c3339cbb096a98d432d17a51f9f (MD5) Previous issue date: 1979
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Single stranded DNA: A new model system for single molecule polymer dynamics
… nearly two decades, fluorescently-labeled double stranded DNA (dsDNA) has been the model system for studying single molecule polymer dynamics in non-equilibrium conditions. However, dsDNA is a semiflexible polymer with markedly different local molecular properties compared to flexible polymer …
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Cloning and characterization of a single-stranded DNA-binding protein of Leishmania major
… of direct head to tail tandem repeats and single dispersed gene copies. In the present study, a single repeat unit of the Leishmania donovani GP63 tandem array was cloned and sequenced. Alignment of the L. donovani GP63 gene sequence with the previously determined GP63 gene sequences from …
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Single -Molecule and Ensemble Fluorescence Studies of Single -Stranded DNA and Replication Proteins
… study three members of the replication system: single-stranded DNA, single-stranded DNA binding proteins, and the sliding clamp and clamp loader proteins. Fluorescence studies have been widely used to study the properties of biomolecules and here we will use both Fluorescence Polarization …
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Single molecule force spectroscopy of single stranded DNA binding protein and Rep helicase
Single-stranded DNA binding (SSB) proteins are essential accessory proteins that protect single-stranded DNA (ssDNA) during genome maintenance. Escherichia coli SSB is a prototypical homo-tetrameric SSB protein that can wrap up to 65 nucleotides of ssDNA in one of its binding modes. Here we present …
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The effect of single-stranded DNA binding protein RPA2 on XPD helicase processivity
… repair (NER). Its function is to unwind double-stranded DNA, allowing access to the bases that connect the strands and code genetic information. Previous work has shown that XPD activity is enhanced by the single-stranded DNA binding protein replication protein A (RPA2). However, the mechanism …
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Binding configurations of single-stranded DNA binding protein and their influence on DNA recombinase
… scenario, this damage results in double-stranded DNA (dsDNA) breaks. Double-stranded DNA breaks are lethal, and difficult to repair, with potential complications from genome rearrangement. To prevent this genetic instability, a cell can utilize a homologous chromosome as a template to …
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The evolutionary impacts of secondary structures within genomes of eukaryote-infecting single-stranded DNA viruses
… they reside because even randomly generated single-stranded RNA/DNA sequences will form stable secondary structures. Nevertheless, with additional genome evolution analyses involving the detection of natural selection, sequence co-evolution, and genetic recombination, it is possible to both …
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CAPILLARY ELECTROPHORESIS ANALYSIS OF SINGLE-STRANDED DNA APTAMERS AND THEIR INTERACTIONS WITH PROTEIN TARGETS AND CARBON DOTS
Aptamers are short, single-stranded oligonucleotides, which fold into particular 3-dimensional structures permitting high-affinity binding to target molecules. In the first part of this work, capillary electrophoresis experiments were conducted to determine the dissociation constant of a unique …
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Molecular, Biochemical, and Evolutionary Analysis of Single-Stranded Dna-Binding Proteins From the Mesophilic Archaeon Methanosarcina Acetivorans
… replication across the three domains of life is single-stranded DNA-binding protein. At the replication fork, these proteins bind to stabilize and protect transiently exposed ssDNA from nucleases during DNA transactions. The ssDNA-binding protein is referred to as SSB in bacteria, whereas in …
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The characterisation of aspects related to the single stranded RNA binding ability of African horsesickness virus nonstructural protein NS2
… part of the family Reoviridae. It is a double stranded RNA virus with ten genome segments that encode seven structural and four nonstructural proteins. AHSV nonstructural protein NS2, encoded by segment eight, is a single stranded RNA (ssRNA) binding protein. The formation of viral inclusion …
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