Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Single Strand Annealing"”.
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THE DNA DAMAGE CHECKPOINT REGULATES MISMATCH REPAIR COMPLEXES DURING SINGLE STRAND ANNEALING PATHWAY
… information. The vast majority of lesions are single-strand DNA breaks caused by physiological processes such as DNA replication or oxidative stress during cellular metabolism. Moreover, single-strand DNA breaks can be converted to DNA double-strand breaks (DSBs), which are the most dangerous …
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Genetic Control of Genomic Alterations Induced in Yeast by Interstitial Telomeric Sequences
… likely occur by the formation of a double-stranded DNA break within the ITS, followed by repair of the break utilizing the single-strand annealing pathway. The point mutations induced by the ITS require the error-prone DNA polymerase zeta. Unlike the terminal inversions, these events are …
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EXAMINING THE ROLE OF THE XAB2 PROTEIN IN HOMOLOGOUS RECOMBINATION
<p>DNA double strand break (DSB) repair is critical to maintain genomic integrity and cell viability. DSBs can occur during the course of cell cycle during replication or transcription, or by exogenous agents such as chemicals or ionizing radiation. For my thesis, I studied homologous recombination …
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The Design and Development of Artificial Zinc Finger Transcription Factors and Zinc Finger Nucleases to the hTERT Locus
… screened in combinatorial pairs in cell-based single-strand annealing assays and gene targeting assays. These screening strategies have pinpointed several ZFN pairs that may be useful in genomic editing of the hTERT locus. Our findings provide guidelines for modifying ZFP architecture to a …
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Analyzing Genomic Studies and a Screen for Genes that Suppress Information Loss During DNA Damage Repair
… can have extremely detrimental effects. Double strand breaks are the most severe form of damage and can be repaired in several different ways or countered by other cellular responses. DNA context is important; cell cycle, chromosomal structure, and sequence all can make DSBs more likely or more …
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Disabling the intrinsic resistome of Mycobacterium tuberculosis: elucidating hierarchies of DNA repair and mutagenesis that undermine current antibiotic efficacy
… the absence of UvrB was compensated by the RecC/Single-strand Annealing pathway. In contrast, DnaE2 deficiency revealed the conditional essentiality of the PadR family transcriptional regulator, MSMEG_2868, under MMC exposure. Importantly, in all cases, results from the Tn screen were validated …
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Gene editing in Aedes aegypti
… (HEs) are selfish elements which catalyze double-stranded DNA (dsDNA) breaks in a sequence-specific manner. The activities of four HEs (Y2-I-AniI, I-CreI, I-PpoI, and I-SceI) were investigated for their ability to catalyze the excision of genomic segments from the Ae. aegypti genome. All four …
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Development of Novel Technologies to Investigate DNA Double-Strand Break Repair Uncovers a Role for the ATM Kinase in Error-Free NHEJ with Implications for Neurodegenerative Diseases
DNA double strand breaks (DSBs) are considered to be the most lethal genotoxic lesion because they can result in chromosomal translocations or result in a major loss of genetic information if repaired incorrectly. To preserve genomic integrity, mammalian cells have evolved a set of complimentary …
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Studies of site-specific DNA double strand break repair in plants
… <p>Multiple pathways are used to repair Double Strand Breaks (DSBs), including Nonhomologous End Joining (NHEJ) and Homologous Recombination (HR) which can take Single Strand Annealing (SSA) or Gene Conversion (GC) pathway. DSB repair in plants has been extensively studied using site-specific …