Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 295 for “"Single Cells"”.
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Visualizing Allele Specific Expression In Single Cells
Single molecule RNA FISH techniques have enabled the quantification of gene expression at the single cell level, revealing significant variability that has been previously obscured by techniques measuring population averages. These techniques, however, have been limited in that they cannot classify …
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Quantification of gene expression in single cells
… of apparently homogeneous cell populations and single cells often give highly divergent results. Cells exhibit varying responsiveness to stimuli and gene expression and they are in many aspects stochastic and unpredictable. We have developed a method to measure gene expression quantitatively in …
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Multiplexed angiogenic biomarker quantification on single cells
Clinical and biomedical research seeks single-cell quantification to better understand their roles in a complex, multi-cell environment. Recently, quantification of vascular endothelial growth factor receptors (VEGFRs) provided important insights into endothelial cell (EC) characteristics and …
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Transcriptional and epigenetic fluctuations in single cells
… in the transcriptional and proteomic state of single cells is a common feature of living systems. The focus of this work is to understand the epigenetic orgin of this heterogeneity. For biochemical noise arising from the intrinsic stochasticity of molecular interactions, we have experimentally …
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Analytical Measurements of Single Cells and Cellular Organelles
… others have the ability to assay the contents of single neurons, far more interesting is the assay single vesicles within those neurons. Since a possible means of neuronal information encoding may be due to the localization of different vesicle populations in different areas of a single cell, this …
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Signal processing and decision making in single cells
Cells are not simple passive observers oblivious to their environment, but sense and adapt to environmental changes in order to thrive. In addition to sensing the presence of signals in the environment, cells can extract information relating to the dynamics and spatial location of these signals and …
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Targeted sequencing : single cells and single strand breaks
… identified as the underlying cause of specific single gene disorders. However, complex diseases still pose a daunting challenge that require both an understanding of cell function as well as how the genome interacts with its cellular environment. Sequencing technologies are now routinely applied …
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Measuring compositional and growth properties of single cells
… or for smaller organisms. The advancement of single-cell measurement techniques and the understanding of cell-to-cell variability have renewed the interest in size characterization. In addition, knowledge of how individual cells grow and coordinate their growth with the cell cycle is of …
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Variability and Dynamics of Apoptotic Events in Single Cells
… variability in the onset of apoptosis between cells in culture, even in clonal populations. Here we developed a reporter (pmDEVD) for caspase activation in single cells. The pmDEVD reporter relocalizes from the plasma membrane to the cytoplasm after caspase activation. We find that the reporter …
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Genome sequencing and phenotypic analysis of single cells in cancer
… sequencing of all genes in the human genome from single circulating tumor cells (CTCs) is both possible and useful clinically. We also describe a statistical framework for evaluating amplification bias in single-cell sequencing and two new methods for enriching CTCs from blood. In the second half …
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Dynamics of gene expression and signal transduction in single cells
… dynamics that are either out of phase among cells or are driven by stochastic cellular components[34]. In this work, we combined time-lapse microscopy, quantitative image analysis and fluorescent protein reporters, which allowed us to directly observe multiple cellular components over time in …
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High-throughput multiplexed transcriptome and protein measurements in single cells
… measurements of transcriptome and proteome in single cells. This method takes advantage of droplet barcoding and hydrogel chemistry to capture mRNAs and proteins in thousands of cells. We report the challenges of acquiring sequencing data from cell gels due to complex mechanisms underlying bead …
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Directed Cell Migration: From Single Cells to Collectively Moving Cell Groups
… driven thermally by Brownian motion, eukaryotic cells move in a particular direction to accomplish designated tasks that are involved in diverse biological processes such as organ development and tumor progression. In this dissertation, I present experiments, analysis, and modeling of directed …
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Generating immunological signatures of diseases by multiplex analysis of single cells
The large diversity of cells that comprise the human immune system requires methods that can resolve the contributions of individual cells to an immunological response. The release of cytokines is one of several important functions carried out by immune cells. Analytical methods that yield multiple …
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Large-area cell-tracking cytometry for biophysical measurements of single cells
Utility of single-cell biophysical markers is often limited due to the low-specificity nature of biophysical markers and lack of existing techniques which can test multiple biophysical characteristics for single cells. To address this challenge, I developed a multiparameter intrinsic cytometry …
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Causal Representation Learning for Predicting Genetic Perturbation Effects on Single Cells
… deepened our understanding of gene regulation in cells. Among these, Perturb-seq has emerged as a powerful technique, enabling high-resolution profiling of transcriptomic responses to genetic perturbations at the single-cell level. Such insights have profound implications for functional genomics …
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Multi-frequency characterization of single cells through CM factors and dielectrophoresis
… to discern the electrical properties of single cells by observing them at multiple frequencies. We first simulate experimental conditions to show that as we increase the number of measured frequencies, we are able to better discriminate among different cells. Furthermore, we use the …
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Quantitative analysis of apoptotic decisions in single cells and cell populations
… for the elimination of damaged or unneeded cells in multicellular organisms. Inactivation of apoptotic cell death is a necessary step in the development of cancer, while hypersensitivity to apoptosis is a factor in degenerative diseases. Many of the molecular components controlling apoptosis …
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Rate of hemolytic antibody production by single cells in vivo in rabbits
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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