Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 1454 for “"Single Cell"”.
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Nanoelectroporation of single cell plants
… yielding a near uniform population of single cells that were significantly larger than their light grown counterparts, forming an attractive target for genetic transformation. In further studies, the single cell sporelings were proven to be metabolically active, through FDA staining for …
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Harmonic Acoustics for Single Cell Manipulation
<p>Technologies that can manipulate single particles and cells in a high precision, high throughput and contact-free manner have long been motivated by applications in materials science, physics, medicine, and the life sciences. However, current single cell manipulation tools, such as atomic force …
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Biophysical approaches to single-cell characterization
High-throughput single-cell RNA-seq (scRNA-seq) is used to describe complex tissues by characterizing transcriptional states of individual cells. Defining a cell’s position, both in regard to tissue margins and its social context, is essential for understanding the intrinsic and extrinsic variables …
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Uncertainty & robustness for single-cell studies
… capable of measuring molecular profiles at single-cell granularity, across thousands or millions of cells, offers unprecedented insight into the form, function, and circuitry of biological systems. At the same time, these technologies present particular statistical and computational …
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Aligning heterogenous single cell assay datasets
Pluripotent stem cells offer strong promise for regenerative medicine but the pluripotent cell state is poorly understood. The goal of this thesis is the development of methods to analyze how the multiple facets of cell state-including gene expression, chromosome contacts, and chromatin …
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A single-cell perspective on infection
… is ultimately determined by a series of cellular interactions between invading pathogens and host immune cells. It has long been understood that these interactions, even when they occur in tissue culture models, give rise to a wide variety of different outcomes, some beneficial to the …
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Inference of single cell profiles from histology stains with the Single-Cell omics from Histology Analysis Framework (SCHAF)
… biology involves an intricate balance between cell-intrinsic processes and interactions between cells organized in specific spatial patterns, which can be respectively captured by single-cell profiling methods, such as single-cell RNA-seq (scRNA-seq), and histology imaging data, such as …
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Multivariate Statistical Analysis In Single Cell Transcriptomics
With technological advances in the last decade, single cell RNA sequencing (scRNAseq) has emerged as an exciting weapon in the modern scientist’s arsenal to unravel cellular heterogeneity. The ability to measure the transcriptome in each individual cell in any given tissue poses challenges galore, …
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Single-Cell Lineage Tracing Of Cancer Metastasis
… lineage recorder with highly efficient single-cell capture of both transcriptional and phylogenetic information. Applying macsGESTALT to a mouse model of metastatic pancreatic cancer, we recover ~380,000 CRISPR target sites and reconstruct dissemination of ~28,000 single cells across …
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Single cell analysis of Keap1-Nrf2 dynamics
… protein which allows Nrf2 to respond to changing cellular conditions. In the basal state, Nrf2 binds to two sites of a Keap1 dimer allowing its ubiquitination in a Cullin-3/Rbx1-dependent manner. In response to electrophiles and oxidants (termed inducers, which bind directly to Keap1) …
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Capillary Electrophoretic Technologies for Single Cell Metabolomics
… neurotransmitters and neuromodulatory compounds. Single cell measurements aid in the discovery of neurotransmitters used by small subsets of neurons that would be diluted below detection limits or masked by ubiquitous compounds when working with larger brain regions. Also, as neurochemistry can be …
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Experimental Design of Single Cell Sequencing Experiments
This thesis addresses limitations of current single-cell sequencing technologies and proposes alternative experimental designs to increase statistical power. Single-cell sequencing enables high-throughput and high-dimensional studies of bio- logical systems. This is particularly useful in …
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Single-cell reporters for inflammatory caspase activity
… of diverse signaling receptors and shape cell fate. Inflammatory caspases mediate the proteolytic processing of inflammatory cytokines and are essential in maintaining immune function, but also lead to disease when deregulated. In order to examine the activity of inflammatory caspases, we …
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Utilising Single-Cell Technology to Investigate Gene Regulation
Since the inception of single-cell sequencing in 2009, experimental and computational technologies have enabled the accurate quantification of gene expression on a cell-by-cell basis. By capturing the transcriptome of thousands of single cells within complex tissues, ranging from organoids to …
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STATISTICAL FOUNDATIONS OF SINGLE CELL OPEN CHROMATIN ASSAYS
Single cell genomic assays are emerging technologies that measure biomolecules in each individual cell. The ability to measure molecules at the single-cell level provides unique opportunities to disentangle complex biological processes. Powered by technological advancements and the ever-decreasing …
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Machine leanring algorithms for single-cell data analysis
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01
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Exploring single cell neurochemistry with multimodal MALDI MS
… upon the active coordination of many different cell types and subtypes at cellular resolutions. While measuring neurochemistry at cellular resolutions is important for understanding emergent properties, such as cognition and memory, analyzing the chemical nature of the brain at the single cell …
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Resolving developmental dynamics using single-cell sequencing data
… disrupted form in disease. Over the past decade, single-cell sequencing has become one of the key technologies to generate high resolution in vivo snapshots to study developmental trajectories. After giving an overview of the current state of single cell technologies and computational methods, I …
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