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Showing 1 to 19 of 19 for “"Sindbis virus"”.
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The Role of RNAi in Mammalian Cells in Response to Sindbis virus infection
Abstract Viruses are obligate intracellular parasites that need to interact with their host in order to replicate successfully. The understanding of this complex interaction between host and virus is essential for developing new therapeutic strategies as viral infections pose a serious challenge in …
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The construction and characterization of recombinant sindbis virus vectors as a platform for anthrax vaccine development
… epitopes responsible for antibody binding. Sindbis virus, the prototypical member of the genus alphavirus, has been extensively used as a vector of foreign gene expression. Several characteristics, including ease of manipulation, high levels of transgenic expression, and lack of …
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The Efficacy of DNA Vaccine Using a Sindbis Virus-based Vector Against Nucleocapsid Protein of Hantaan Virus
"Hantaan virus (HTNV) is a causative agent of hemorrhagic fever with renal syndrome (HFRS). More than 100,000 cases of HFRS are reported yearly, with a mortality rate of between 2% and 10%. But, there is no effective and safe vaccine against HFRS. Even though neutralizing antibodies against the …
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Sindbis virus usurps the cellular HUR protein to stabilize its transcripts and promote infections of mammalian and mosquito cells
Members of the genus Alphavirus are recognized as significant human pathogens. Infection of vertebrate hosts often results in febrile illness and occasionally severe encephalitis. The archetypical alphavirus is Sindbis virus, which we have utilized in these studies. The genomic and subgenomic RNAs …
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Epidemiology of Sindbis fever in South Africa and development of a real-time pan-alphavirus PCR assay for the detection of Sindbis and other medically important alphaviruses
The genus Alphavirus in the family Togaviridae consists of approximately 30 single stranded, positive-sense RNA viruses which cause diseases ranging from mild, febrile illness with rash and arthritis to life-threatening encephalitis. Sindbis virus is the prototype virus for this genus and is the …
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Autophagy in Antiviral Immunity
… inhibition in a mouse model of herpes simplex virus type I (HSV-1) encephalitis, investigated the role of autophagy in protection against Sindbis virus infection of the central nervous system, and identified novel host genes involved in targeting viral proteins to the autophagy pathway. We …
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Isolation and characterisation of extracellular vesicles in baculovirus infection of insect cells
The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an insect-specific virus. Its infection can be studied in Spodoptera frugiperda. It has a double-stranded DNA genome and a biphasic lifecycle, producing budded virus (BV) to infect neighbouring cells and occlusion …
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Infection and Dissemination of TaV-GFP Tagged Sindbis in Aedine Mosquitoes and Cell Lines
Arthropod-borne-viruses (arboviruses) pose a global threat due to their ability to be transmitted by hematophagous insects to vertebrate hosts resulting in a range of serious infectious diseases. Sindbis virus (SINV) is the prototype arbovirus of the genus Alphavirus in the family Togaviridae. The …
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Isolation and characterization of dengue virus membrane-associated replication complexes from Aedes aegypti
Ultrastructural studies of flavivirus replication have long observed proliferation of host membranes. Membrane-bound replication compartments have recently been isolated and characterized from flavivirus-infected mammalian cells, providing insight into the morphology, organelle of origin, and …
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Antiviral activities of selected Chinese medicinal plants
… 22 plant families were assayed for inhibition of Sindbis and murine cytomegalovirus infections in mammalian cell cultures. Sixteen of the species showed antiviral activity. Elsholtzia ciliata (Thunb.) Hyland of the mint family (Lamiaceae) showed the highest activity. It has more than one active …
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The inducible antiviral immune response of Drosophila melanogaster
… Fliegen, welche entweder mit dem Drosophila C Virus (DCV), dem Flock- house Virus (FHV) oder dem Sindbis Virus (SINV) infiziert waren. Infektion mit diesen drei positiv orientierten Einzelstrang RNS Viren führte zu einer starken transkriptionellen Antwort, welche deutlich virusspezifische …
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Mosquito Species Abundance, Diversity and Arbovirus Prevalence across Different Land-use in The Gambia
Mosquito-borne arboviruses represent an escalating public health threat across sub-Saharan Africa, yet surveillance remains limited in many countries, including The Gambia. This study investigates mosquito species abundance and diversity, as well as the arbovirus prevalence across human, …
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Two Odorant-Binding Protein Genes in Mosquitoes: Comparative Genomics, Expression, and Function
… the function of these Obp genes more directly, a Sindbis virus based expression system is established to knockdown the two Obp gene orthologs in Aedes aegypti. The effective knockdown of Obp1 and Obp7 genes (8 and 100-fold, respectively) is accomplished in female mosquito olfactory tissues. The …
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Evidence for RNA interference as an antiviral immune response to alphavirus infection in the mosquitoes Anopheles gambiae and Aedes aegypti
Mosquito-borne viruses remain a serious threat to public health worldwide. A lack of effective vaccines and vector control programs have led to the geographical expansion of the vectors and their associated agents, thereby increasing the percent of the global population that are affected. The …
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Evaluation of arthropod-borne virus recombination potential and phenotype: In vitro and in vivo analysis of flavivirus and alphavirus model systems
… whereby positive sense single stranded RNA viruses exchange segments of genetic information. Recent phylogenetic analyses of naturally occurring recombinant flaviviruses have raised concerns regarding the potential for the emergence of virulent recombinants either post-vaccination or …
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A Role for Interferon Stimulated Gene-15 (ISG15) During Chikungunya Virus Infection
… Stimulated Gene-15: ISG15) During Chikungunya Virus Infection</p><p>By</p><p>Scott William Werneke</p><p>Doctor of Philosophy in Biology and Biomedical Science</p><p>(Immunology)</p><p>Washington University in St. Louis, 2013</p><p>Professor Deborah J. Lenschow, Chairperson</p><p>Chikungunya …
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Autophagy in Zellweger Syndrome Spectrum Disorder and Cancer
… PEX13 is required for selective autophagy of Sindbis virus (virophagy) and of damaged mitochondria (mitophagy), and that disease-associated PEX13 mutants I326T and W313G are defective in mitophagy. The selective mitophagy function of PEX13 is shared with another peroxin family member PEX3, but …
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Mosquito Odorant Receptors: C-terminal Motifs, Subfamily Expansion, and Function
… for inference of receptor function. Recombinant Sindbis viruses were used to knockdown mRNA levels of these receptors. GFP-expressing recombinant Sindbis viruses were shown to infect chemosensory tissue. Additional viruses containing fragments of receptor genes were found capable of lowering …
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Utilizing Proteomic Techniques to Discover Host Protein Interactions with the E1 Glycoprotein of Venezuelan Equine Encephalitis Virus (VEEV) for Anti-Viral Discovery
Venezuelan equine encephalitis virus (VEEV) is an alphavirus that causes disease in humans and equines eliciting both an agricultural and public health threat. In humans, the disease typically presents as a febrile illness with common signs of fever and malaise. Four to fourteen percent of …