Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Sindbis"”.
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Epidemiology of Sindbis fever in South Africa and development of a real-time pan-alphavirus PCR assay for the detection of Sindbis and other medically important alphaviruses
… and arthritis to life-threatening encephalitis. Sindbis virus is the prototype virus for this genus and is the most widely distributed alphavirus globally. Little epidemiological data exists for this virus due to inadequate surveillance efforts in most countries, and it is suspected that Sindbis …
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The Role of RNAi in Mammalian Cells in Response to Sindbis virus infection
… in the interaction between an RNA virus (Sindbis virus) and its mammalian host cell. A sensitive image–based viral replication assay was developed to follow Sindbis virus replication in HEK293C cells and the main anti-viral innate responses to virus infection described. Virus replication …
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Infection and Dissemination of TaV-GFP Tagged Sindbis in Aedine Mosquitoes and Cell Lines
… in a range of serious infectious diseases. Sindbis virus (SINV) is the prototype arbovirus of the genus Alphavirus in the family Togaviridae. The purpose of this study was to investigate the use of a fluorescent tagged reporter virus in both in vitro and in vivo environments. The fluorescent …
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The construction and characterization of recombinant sindbis virus vectors as a platform for anthrax vaccine development
… epitopes responsible for antibody binding. Sindbis virus, the prototypical member of the genus alphavirus, has been extensively used as a vector of foreign gene expression. Several characteristics, including ease of manipulation, high levels of transgenic expression, and lack of …
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The Efficacy of DNA Vaccine Using a Sindbis Virus-based Vector Against Nucleocapsid Protein of Hantaan Virus
… strategy was applied to HTNV in this study. Sindbis virus-based expression vector was carefully designed and constructed, in order to induce the transient high level expression of target gene. In the case of DNA vaccination by plasmid encoding nucleocapsid gene, a single dose injection of …
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Sindbis virus usurps the cellular HUR protein to stabilize its transcripts and promote infections of mammalian and mosquito cells
… encephalitis. The archetypical alphavirus is Sindbis virus, which we have utilized in these studies. The genomic and subgenomic RNAs of Sindbis virus strongly resemble cellular mRNAs as they are capped at their 5’ ends and polyadenylated at their 3’ termini. These features allow the viral RNAs …
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Antiviral activities of selected Chinese medicinal plants
… 22 plant families were assayed for inhibition of Sindbis and murine cytomegalovirus infections in mammalian cell cultures. Sixteen of the species showed antiviral activity. Elsholtzia ciliata (Thunb.) Hyland of the mint family (Lamiaceae) showed the highest activity. It has more than one active …
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Bionomics of vector-borne diseases in sites adjacent to lakes Victoria and Baringo in Kenya
… and 18 wildlife species. Further detection of Sindbis and Bunyamwera viruses was done on blood-fed mosquito homogenates by Vero cell culture and RTPCR in Culex, Aedeomyia, Anopheles and Mansonia mosquitoes from Baringo that had fed on humans and livestock. In TBPs assay, 585 tick pools were …
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Overexpression of Serum Response Factor in Astrocytes Improves Neuronal Plasticity in a Model of Fetal Alcohol Spectrum Disorders
… OD plasticity in alcohol-treated ferrets using a Sindbis viral vector to express a constitutively active form of SRF during the period of monocular deprivation. Using optical imaging of intrinsic signals and single unit recordings we observed that overexpression of a constitutively active form of …
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Autophagy in Antiviral Immunity
… the role of autophagy in protection against Sindbis virus infection of the central nervous system, and identified novel host genes involved in targeting viral proteins to the autophagy pathway. We found that the HSV-1 encoded neurovirulence protein ICP34.5 interacted with the host autophagy …
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Isolation and characterisation of extracellular vesicles in baculovirus infection of insect cells
… (RNAi). In Drosophila melanogaster, following Sindbis virus infection, an RNAi signal was reportedly spread via extracellular vesicles (EVs). Extracellular vesicles incorporate proteins and nucleic acids of the cell they originate from. Proteins involved in their biogenesis are frequently used …
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Advances in Two-Photon Fluorescence Microscopy for High-Resolution Anatomical and Functional Imaging of Cell Populations in the Intact Brain
… Zellpopulationen entwickelt. Insbesondere wurden Sindbis- und Lentiviren zum Transfer und der gerichteten Expression genetisch kodierter Fluoreszenzindikatoren in Neuronen eingesetzt. Weiterhin wurde eine Methode zur spezifischen Färbung von Astroglia entdeckt und die transgene Expression von …
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Mosquito Odorant Receptors: C-terminal Motifs, Subfamily Expansion, and Function
… for inference of receptor function. Recombinant Sindbis viruses were used to knockdown mRNA levels of these receptors. GFP-expressing recombinant Sindbis viruses were shown to infect chemosensory tissue. Additional viruses containing fragments of receptor genes were found capable of lowering …
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Isolation and characterization of dengue virus membrane-associated replication complexes from Aedes aegypti
… staining for dsRNA between DENV and Sindbis virus (SINV). We also evaluated replication of DENV mutants in the DENV-resistant transgenic mosquito strain known as Carb77 and whether mutations in DENV genome sequence lead to evasion of the enhanced RNAi response of Carb77 mosquitoes. …
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Cross-reactivity among alphaviruses provides insight into viral emergence and novel defense strategies
… while Old World alphaviruses, such as Sindbis, chikungunya, and Mayaro viruses (SINV, CHIKV, and MAYV, respectively) present with an incapacitating polyarthralgia that can persist for years following initial infection. To date, the most effective means of controlling these arboviral …
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The inducible antiviral immune response of Drosophila melanogaster
… (DCV), dem Flock- house Virus (FHV) oder dem Sindbis Virus (SINV) infiziert waren. Infektion mit diesen drei positiv orientierten Einzelstrang RNS Viren führte zu einer starken transkriptionellen Antwort, welche deutlich virusspezifische Indukti- onsmuster zeigte. Um die biologische Signifikanz …
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Mosquito Species Abundance, Diversity and Arbovirus Prevalence across Different Land-use in The Gambia
… (two pools), Bunyamwera virus (one pool) and Sindbis virus (two pools), representing the first confirmed detection of these arboviruses in field-collected mosquitoes from The Gambia. Interestingly, all five pools were identified during the late rainy season across inland sylvatic, …
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Characterization of a Newly Identified Alphavirus, Eilat Virus
… infection with EILV reduced virus replication of Sindbis, chikungunya, eastern, western and Venezuelan equine encephalitis virus by 100- to 10,000-fold indicating that EILV virus may alter host susceptibility to superinfection and therefore the ability to transmit pathogenic alphaviruses. Taken …
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Investigating the effects of pH on alphaviral E3-E2 glycoprotein association, organization, and cellular tropism
… receptor for the prototypic alphavirus Sindbis (SIND), on cellular targets. We observed a significant decrease in entry of VEEV versus control Ross River (RRV) virus that does not utilize NRAMP2. This inhibition can be rescued with binding to heparan sulfate by VEEV retaining E3. …
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Two Odorant-Binding Protein Genes in Mosquitoes: Comparative Genomics, Expression, and Function
… the function of these Obp genes more directly, a Sindbis virus based expression system is established to knockdown the two Obp gene orthologs in Aedes aegypti. The effective knockdown of Obp1 and Obp7 genes (8 and 100-fold, respectively) is accomplished in female mosquito olfactory tissues. The …
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