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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Silver Staining"”.
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Axonal Damage in Repetitive Concussive Traumatic Brain Injury: Characterization and Contributing Factors
… by amyloid precursor protein, neurofilament, and silver staining abnormalities in the absence of gross structural changes or cell loss. Microglia and astrocytes both became activated and were prominent in injured white matter by 7 days. Behaviorally, injury resulted in acute Morris Water Maze …
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Gel-based proteomic comparison of two strains of Propionibacterium freudenreichii ssp. shermanii
… concentration greatly improved spot resolution. Silver staining achieved higher spot counts than Coomassie staining. The influence of the number of lysis cycles on protein extraction was marginal, while lower protein sample loads produced superior 2-D gels. Results show that data from 2-DE …
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Effect of irradiation of head and neck tumours on the proteins' composition of the enamel salivary pellicle
… analyzed by electrophoretical separation and silver-staining as well as immunodetection of alpha-amylase, IgA and acidic-PRPs. Conclusively to our results, irradiation of the head and neck region seems to affect the quantity but not the chemical composition of the enamel salivary pellicle.
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Molecular analysis of the dry rot fungus Serpula lacrymans
SDS-PAGE/silver staining and western blotting were used to investigate the molecular profile of a PBS soluble extract from standard mycelium profile of S. lacrymans FPRL 12C. Both techniques enabled a reference profile for this organism to be established. A polyclonal antiserum raised against …
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Steroid Stimulated Protein Synthesis in Fish Cell Cultures
… any prominent new protein bands as detected by silver staining polyacrylamide gels or fluorography.
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Chromosomal localization of a proinsulin transgene inserted with a transposon-based vector into Japanese quail, Coturnix coturnix
… fluorescent in situ hybridization, and silver staining. Teflon-coated coverslip slides were created to facilitate laser microdissection of avian chromosomes for DNA amplification and nucleotide sequencing. Transgenic G2 Japanese quail produced in Dr. Richard Cooper¡¦s laboratory were …
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In vitro Expression of Human Zona Pellucida Protein 3
… gel electrophoresis (SDS-PAGE) with silver staining, Western blot analysis using a specific anti-ZP3 antibody, and by a metabolic labelling study using <sup>3</sup>H-galactose. Several bands including a band at 65 kDa, were observed in both non-transfected and transfected medium by …
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ΣΥΓΚΡΙΤΙΚΗ ΜΕΛΕΤΗ ΤΗΣ ΧΟΡΙΟΓΕΝΕΣΗΣ ΣΕ ΕΝΤΟΜΑ ΟΙΚΟΝΟΜΙΚΗΣ ΣΗΜΑΣΙΑΣ
… DIMENTIONAL ELECTROPHORESIS,AUTOFLUOROGRAPHY, SILVER STAINING METHOD OF GELS, IMMUNOCALIZATION OF PEROXIDASE WITH WESTERN BLOTTING AND DOUBLE ANTIBODY STAINING, PEROXIDASE LOCALIZATION IN THE LIGHT AND ELECTRON MICROSCOPE LEVEL USING THE O-DIANISIDINE AND DIAMINOBENZIDINE (DAB) METHODS, …
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Metabolic Regulation of Cellular Signaling
… gel electrophoresis (SDS-PAGE) and silver staining. The results indicate the presence of numerous CaM-binding proteins that also bind CoA and are thus potentially metabolically regulated. In a targeted approach, we tested the ability of aberrant glucose signaling to regulate the …
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Chromosomal Evolution Among North American Microtine Rodents
… 14 species of Microtus. Three differential staining procedures were utilized: C-banding, G-banding, and silver staining. Results of the C-banding analyses indicated that autosomal heterochromatin occurs primarily at centromeric regions, although several species do have long arm interstitial …
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Obesity and neuroinflammation : the impact of adipocyte-derived extracellular vesicles on microglial cell signalling
… measured vesicle uptake in microglial cells. Silver staining was done to detect total protein content within EV samples. Western blotting analysed pNF-κB expression in microglial cells after co-culturing with AT-EVs.Results: HMC3 and BV2 cells express a morphologically inflammatory phenotype …
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Characterization of native and bypass human coronary artery plaque deposits from the same heart: investigation of the chemical form of calcium in human coronary artery plaque deposits
… using x-ray absorption and light microscopy with silver staining. Finally, chromatographic methods (CEC and APCI-MS) showed that only 40 % or less of the plaque in native or bypass arteries consisted of cholesterol and its esters and the remaining, 60% were composed of proteins, fatty acids, and …
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Purification and Characterization of Proteoglycan from Bovine Aortic Endothelial Cells Conditioned Media, and its Interaction with Basic Fibroblast Growth Factor (bFGF)
… of approximately 5 x 10⁵ Da. SDS-PAGE with silver staining indicated that BAE PG had two core proteins with estimated sizes of 300kDa and 320kDa, which corresponded to the core protein of PG-I and PG-II respectively. Western blotting with anti-perlecan primary antibody recognized the core …
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Langfristige Entwicklung Zytostatika-induzierter Nierenfunktionsstörungen bei pädiatrisch-onkologischen Patienten
… kidney disease can be detected. SDS-PAGE with silver staining is a method which is able to detect tubular or glomerular abnormalities in a more sensitive way than serum creatinin or creatinin clearance does. Laser densitometry of the protein patterns allows documentation and follow-up of …
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Enzyme activities associated with gonadotropic hormones
… SDS-PAGE with detection by autoradiography and silver staining. The purified receptor was found to have undergone proteolysis during the purification procedure, presumably following incubation with the hCG affinity matrix. Recent reports of the presence of homologous amino acid sequences in the …
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Molecular, cytogenetic, anatomical, and morphological variations associated with polyploids of prairie cordgrass
… has resulted in variation in the number of silver stained NORs (AgNORs) on chromosomes at metaphase. The distribution patterns of AgNORs in metaphase cells of tetra- and octoploids appears to reflect changes in ploidy. Two populations of tetra- and octoploids have the number of AgNORs …