Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 26 for “"Signal Peptides"”.
-
Archaeal signal peptides and cell surface structures
… unfold. Integral to this process are the various signal peptidases. Two types of archaeal signal peptidases are identified thus far: signal peptidase I (SPI) and the preflagellin peptidase. SPI is responsible for processing the majority of secreted proteins. Sequence analysis of archaeal SPI …
-
Incorporation of tumor antigen epitopes into cytokine signal peptides enhance the efficacy of immuno-gene therapy
… expression of antigen epitopes within cytokine signal peptides. We first investigated the possibility of modifying cytokine signal peptides with antigen epitopes without aborting function. We inserted the genes encoding the MHC class I restricted antigenic epitopes of chicken ovalbumin (OVA), …
-
Interactions between the TatBC complex and Tat signal peptides during protein transport by the bacterial Tat pathway
… are targeted to the Tat machinery via N-terminal signal peptides that have a tripartite structure, with a polar n-region, a hydrophobic h-region and a polar c-region. They critically contain an almost invariant twin-arginine (RR) motif within the n-region that is essential to trigger Tat …
-
Investigating the Unique Mechanism of Action of CADA Analogs for Down-Modulating CD4, Sortilin, and ACE2
… glutamine (Q-15) and proline (P-20) in the hCD4 signal peptide (SP). It has been proposed that CK147 may bind with the hCD4 signal peptide through a dipole-dipole interaction between the N,N-dimethylaminobenzenesulfonyl side-arm of CK147 and the amide side chain of glutamine (Q-15) of hCD4 SP, …
-
Probing the role of Tat proofreading chaperones in the assembly of molybdoenzymes
… targeted to this system by N-terminal signal peptides which bear a conserved SRRxFLK motif. Tat dependent proteins often contain redox factors that must be inserted prior to translocation. The E. coli Tat substrates trimethylamine Noxide<br/>reductase (Tor) and periplasmic nitrate …
-
Polarised secretion of leukaemia inhibitory factor
… The transcripts LIF-D and LIF-M encode different signal peptides, which in mouse have been associated with differential localisation of the mature protein. LIF-D is associated with a freely diffusible protein, whereas the LIF-M is associated with the extracellular matrix. The polarity of LIF …
-
Parallel effects of signal peptide hydrophobic core modifications on cotranslational translocation and posttranslational cleavage by purified signal peptidase
… amino terminal extension sequence known as the signal peptide. Signal peptides contain a central core of uncharged amino acids that are usually hydrophobic in nature. The hydrophobic core of the parathyroid hormone (PTH) signal peptide is composed of 12 contiguous hydrophobic amino acids. …
-
Engineering geobacillus thermoglucosidasius for efficient production of fuels and chemicals
… To improve heterologous protein secretion, 25 signal peptides from G. thermoglucosidasius C56-YS93, predicted by SignalP 4.1 server, were screened and characterized for different secretory target proteins. Three thermostable hydrolase for biofuel production were selected: α-amylase (amyE) from …
-
Antifreeze Glycoproteins of Two Unrelated Polar Fishes: Gene Structure, Organization and Evolution
… and northern AFGP genes also have different signal peptides, different intron-exon organizations, different spacer sequences thus are the processing of the polyprotein precursors, and drastically different codon patterns for the repetitive AFGP coding sequence. This molecular evidence in …
-
Carbonic anhydrase from Methanosarcina thermophila: proposal of a new class of carbonic anhydrases and putative roles for the enzyme in anaerobic acetate catabolism
… residues with properties characteristic of signal peptides in secretory proteins. The deduced amino acid sequence has no significant identity to any known CAs, but has, among others, 35% sequence identity to the first 197 deduced N-terminal amino acids of a proposed …
-
Subcellular Localization of Tobacco Salicylic Acid Binding Protein 2 in Plants.
… leaves. SABP2 processes the mobile defense signal, methyl salicylic acid generated in plants resisting microbial infection. The precise localization of SABP2 in plants is not known. SABP2 has not been shown to have any targeting signal peptides. This study was designed to determine …
-
Functional analyses of Plasmodium Falciparum primary metabolic genes
… nuclear-, and apicoplast-targeting signal peptides in CHO-K1 cells. Taken together, this study has validated a plausible interactive network of primary metabolic plasmodial genes.
-
Larval Hemolymph Proteins and Physiological Role of Prophenoloxidases in Anopheles Gambiae
… and cellular defense responses as well as immune signal transduction, including pattern recognition receptors, serine proteases, serpins, antimicrobial peptides. In the present study, we collected hemolymph samples from water- and E. coli-pricked A. gambiae larvae. The samples were separated on …
-
Early Events in the Biosynthesis and Subcellular Consignment of Secretory and Membrane Proteins
… proteins have been examined in light of the signal hypothesis. Model systems for the study of the biosynthesis of these different classes of proteins, as well as for the study of specific events common to both, have been developed. Their common as well as distinctive features have been probed …
-
Giardia trophozoite-secreted proteins and their effects on intestinal epithelia
… proportion encoded by genes annotated to have signal peptides, known virulence factors such as the Cathepsin B cysteine proteases and Variable Surface Proteins, scavenging proteins such as an extracellular nuclease and a high proportion of hitherto hypothetical proteins and proteins of unknown …
-
Structural and Molecular Basis for Multifunctional Roles of Cellular Proteases
… were first known for their roles in processing peptides such as signal peptides. Recent discoveries have begun to unravel their multifunctional roles in many other biological processes including blood pressure regulation and virus infection. However, the knowledge gathered thus far is still …
-
Mapping protein-protein interactions in the <i>Escherichia coli</i> Twin Arginine Translocase
… complex which binds substrates through their signal peptides, with TatA being recruited to form an oligomeric structure that facilitates protein transport. Current models suggest that TatA oligomers locally disrupt membrane lipids allowing the substrate to move from the cytoplasm to the …
-
Identification and characterisation of proteases in Mycobacterium tuberculosis
… identity and are all encoded with putative signal peptides, suggesting that they are translocated across the cytoplasmic membrane. One member, mycP1, was selected for further study. The gene product, mycosin-1, was 30-35% identical to bacterial subtilisin-like serine proteases and contained …
-
Characterization of Chitinase Activity and Gene Expression in Muskmelon Seeds
… to secretory pathways because they possess signal peptides. Southern blotting suggested that there is at least one additional gene similar to Cmchi1 in the muskmelon seed genome, while there is only one copy of Cmchi2. Northern blotting analysis showed that both Cmchi1 and Cmchi2 are …
-
The discovery and functional assessment of novel peptides from the skin secretions of frogs
… encoding biosynthetic precursors of the three peptides were cloned through 3’RACE PCR. Briefly, degenerate primers were designed based on the 5’-untranslated regions of other dermaseptin cDNAs.<br/><br/><br/>Bioinformatic tools were used to reveal the open-reading frames and putative signal …
Page 1 of 2