Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Side population"”.
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Stem cells in the adult heart - 3D culture, isolation of side population cells and search for a stem cell niche
… levels increased over time. In Study II Side Population progenitors were isolated from the left human atria. In Study III the distribution of label retaining cells was investigated, throughout the adult rat heart and a region in the Atrio Ventricular junction (AVj) was proposed as a …
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Somatic Stem Cell Populations and Studies on the Functional Role and Regulation of ABCG2
… can also efflux Hoechst 33342, resulting in a side population phenotype when stained cells are sorted by FACS. Recent studies have suggested that Abcg2 may be a marker for stem and progenitor cells. This paper presents experiments that were undertaken to further evaluate the functional role and …
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Notch Signaling Is Important in the Survival, Proliferation, and Self-Renewal of the Putative Breast Cancer Stem Cell Population
… affected.</p><p>The stem cell-like population was isolated using several different methods including side population, mammosphere formation and aldehyde dehydrogenase activity. Notch pathway activity was analyzed in CSCs derived from cell lines and primary tumors of different breast …
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Exploring the heterogeneity of the hematopoietic stem and progenitor cell pool in cord blood
… for the simultaneous definition of the Hoechst Side Population (SP), Aldehyde Dehydrogenase activity (ALDH) and the expression of the surface protein CD34 and secondly for the definition of viable and apoptotic cells in the ALDH and CD34 positive populations respectively. Finally, we screened …
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Isolation and characterization of primitive hematopoietic cells
Side-population (SP)cells are selected based on the rapid efflux of the fluorescent DNA-binding dye Hoechst 33342. Murine BM SP cells had long-term repopulating activity and proved negative for the antigen CD34. Such SP cells were detected in rats, pigs, monkeys and human cell sources. Within SP …
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Targeted magnetic hyperthermia for lung cancer
… Cancer stem cells (CSCs) are a sub-population of stem-like cells that are thought to be responsible for tumor drug resistance and relapse. We determined the susceptibility of CSCs to magnetic hyperthermia. Multiple assays for CSCs, including side population phenotype, aldehyde …
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Investigating heterogeneity and mechanisms of resistance in paediatric B-cell non-Hodgkin lymphoma
… The aim of this thesis was to identify cell populations that are driving therapy resistance in order to develop targeted treatments for relapsed and therapy-refractory patients. Utilising samples obtained from patients enrolled to the Inter-B-NHL ritux 2010 clinical trial, we established 5 …
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Charakterisierung humaner kurz-zeitrepopulierender Blutstammzellen im NOD/SCID-ß2m-/- Xenotransplantationsmodell
… wurden mPB Zellen in CD34+Thy1+ und CD34+Thy- Populationen aufgetrennt und in Xenotransplantationsexperimente durch serielle FACS Analyse der Knochenmaraspirate das Kurzzeitrepopulationsverhalten dieser Populationen in NOD/SCID-ß2m-/- Mäusen untersucht. Dabei konnte gezeigt werden, daß STRC …
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The role of the tumour microenvironment components in cancer cell behaviour and drug response
… presence of CSCs in these tumours. FACS-isolated side population cells exhibited high levels of cancer stem cell markers, self-renewal markers and drug resistance proteins and were associated with increased drug resistance versus cancer cells. In order to measure how ECM proteins affect …
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Gentherapeutische Ansätze zur Behandlung der Muskeldystrophie vom Typ Duchenne
… Die Resultate zeigten, dass die Muskel SP-Zellpopulation mit und ohne Schädigung des Muskelgewebes nicht von Knochenmarkzellen regeneriert wird. Diese Beobachtung deutet auf einen nicht-hämatopoetischen Ursprung dieser Zellen hin. <br> Ein weiteres Ziel dieser Arbeit war es, eine "universelle" …