Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Sialyltransferases"”.
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REGULATION OF GLYCOSYLATION IN MAMMALIAN CELL LINES: INSIGHTS FROM STRUCTURAL GLYCOMICS, ANALYSIS OF GLYCOSYLATION-RELATED GENES AND FUNCTIONAL RNAI SCREENS
… BHK-21 and CHO-K1 cells. Overexpression of three sialyltransferases led to significant enhancement of EPO sialylation in HEK293, COS-7, 3T3 and NS0 cells. The other glycogenes involved in upstream reaction steps could not synergize the effect of these sialyltransferases to further enhance …
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Investigation of inhibitors of polysialyltransferase as novel therapeutics for neuroblastoma. Development of in vitro assays to assess the functionality and selectivity of novel small-molecule inhibitors of polysialyltransferases for use in neuroblastoma therapy
… wider sialyltransferase family (α-2,3-and α-2,6-sialyltransferases) was confirmed using differential lectin staining. These results demonstrated that small molecule inhibitors as STX is possible and provides suitable in vitrocell based assays to discovery more potent derivatives.
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Investigation of Haemophilus somnus Virulence Factors: Lipooligosaccharide Sialylation and Inhibition of Superoxide Anion Production
… (hsst-I) and -2 (hsst-II) had BLASTx homology to sialyltransferases, while ORF-3 (neuAhs) had BLASTx homology to CMP-sialic acid synthetases. These ORFs were amplified by PCR and cloned into the expression vector pCWOri+. Thin layer chromatography of the hsst-I gene product showed this …
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Identification of tumour-associated gycoproteins recognised by the lectin from Helix pomatia in breast cancer
… (ppGalNAc T1,T2, T3 and T6) and alpha 2,6 sialyltransferases (ST6GalNAc I and II) in the breast cell lines, but no correlation between the expression levels of mRNA of these enzymes and HPA binding was found in this study. The results from the present study show that, as in CRC cell lines, …
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Metabolic Pathways for Natural and Unnatural Sialic Acids
Carbohydrates, along with lipids, nucleic acids, and amino acids constitute the four main building blocks of life. This thesis will focus on sialic acid, a unique, nine-carbon sugar that is critically important in humans. In Chapter 1, I will introduce sialic acid biology and the many diseases that …