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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Sialic Acids"”.
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Metabolic Pathways for Natural and Unnatural Sialic Acids
Carbohydrates, along with lipids, nucleic acids, and amino acids constitute the four main building blocks of life. This thesis will focus on sialic acid, a unique, nine-carbon sugar that is critically important in humans. In Chapter 1, I will introduce sialic acid biology and the many diseases that …
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Development of Chemical Tools to Discover and Characterize Sialic Acid Mediated Interactions
… involve a class of carbohydrates known as sialic acids. The presence of sialic acid on a glycan can recruit specific sialic acid binding partners. Alternatively, sialic acids can mask underlying glycan epitopes, thereby preventing protein binding. There are several challenges in studying …
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Regulation of Sialic Acid Metabolism
In bacteria, sialic acids act as virulence factors for certain pathogens, and have been determined to play an important role in the establishment of many disease states. Therefore, studying the intricacies of sialic acid metabolism and its regulation provide a foundation for studying several …
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Design, Synthesis and Biological Evaluation of Inhibitors of Polysialyltransferases PST and STX. Design, synthesis and biological evaluation of a range of N-modified mannosamines, sialic acids and analogues from in silico screening as inhibitors of PolySia-NCAM biosynthesis with anti-migration activity.
… N-acylmannosamine analogues, thio-linked CMP-sialic acid analogues, N-acyl modified sialic acids and compounds incorporating elements of both approaches. Novel methodology development in the synthesis of many of the compounds is described, notably a novel route to N-acyl sialosides. In …
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Role of Neuraminidase-mediated Desialylation in Microglial Activation
… which is the removal of cell surface sialic acid residues by enzymes called sial- idases or neuraminidases. Sialic acids are 9-carbon, negatively charged monosaccharides that normally form the terminal residue of the mammalian cells’ glycocalyx. A murine microglial cell line and …
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Production and characterisation of novel recombinant antibody fragments against sialic acid
Sialic acids are a family of acidic monosaccharides that typically reside as terminal moieties on N- and O-linked glycans. These sugars are actively involved in a plethora of biological phenomena, ranging from cell-cell adhesion and recognition, intracellular signalling events, pathogen attack, …
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New Carbohydrate-Based Anti-Cancer And Anti-Bacterial Vaccines
… was developed for the quantification of various sialic acids expressed by cells and used to analyze the efficiency of N-phenylacetyl-D-mannosamine (ManNPhAc) to metabolically glycoengineer SKMEL-28 cancer cell. In specific, after cancer cells were cultured with ManNPhAc, the cells were treated …
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Immunomodulatory Roles of the Lysosomal Sialidase Neuraminidase 1
<p><strong>Background</strong> Sialic acids are key sugar moieties located at the non-reducing terminals of glycan chains on glycoproteins and glycolipids. By virtue of their location, they influence the functions and biochemical properties of the macromolecules they are bound to. Removal of sialic …
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Methoden zur asymmetrischen nucleophilen Glyoxylierung
Chiral alpha-ketoacids and their derivatives play an important role in organic synthesis and are widely used as inhibitors of both proteolytic enzymes as well as inhibitors of leukotriene A4 hydrolase. Furthermore they are an integral part of important biologically active ulosonic- and sialic …
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Downstream processing of recombinant and endogenous proteins from livestock milk
… that resulted from the -carboxylated glutamic acids and the sialic acids.
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Sex-specific gene expression and its effect on osteoclast maturation
… and morphology, but removal of these specific sialic acids does not appear to more strongly impact one sex over the other. In this study, we bring light to variance in gene expression between male and female osteoclasts, which may in turn be useful in therapeutically modulating cell behavior as …
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Synthesis of inhibitors of polysialyltransferases PST and STX. Development of routes to synthesis, preparation and purification of carbohydrate and carbacycle-based potential inhibitors of the polysialyltransferase enzymes PST and STX
PolySialic acid (polySia) is a linear carbohydrate homopolymer of α- 2,8-linked sialic acids and a posttranslational modification of NCAM (neural cell adhesion molecule), biosynthesized by combined action of two polysialyltransferase enzymes, ST8SiaIV(PST) and ST8SiaII(STX). PolySia alters …
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An investigation of the regulation of Sialoadhesin ligands on CD4+ T cells and its potential significance in Systemic Lupus Erythematosus
Sialoadhesin (Sn) is the prototype of Sialic acid binding Immunoglobulin-Like Lectins(SIGLECs). Sn is expressed on macrophages from metalophilic zone and subcapsular sinus in spleen and lymph nodes respectively. Under inflammatory conditions, Sn expression is upregulated on macrophages. Sn was …
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Molecular and Biochemical Characterisation of SiaP as a Sialic Acid Binding Protein Component of a TRAP Transporter of Sialic Acid.
Sialic acid utilisation plays an important role in the growth and persistence of the obligate human mucosal pathogen Haemophilus influenzae, which causes respiratory tract infections, septicaemia and meningitis. Like many other bacteria, H. influenzae can use host-derived sialic acids as carbon, …
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Interaction of the porcine reproductive and respiratory syndrome virus with the macrophage-specific lectin sialoadhesin
… the porcine sialoadhesin (pSn) shows clear sialic acid binding activity. A first objective was to verify if the R116 amino acid (aa) in the N-terminal V-set domain of pSn is important for its sialic acid binding capacity, as has been shown for mSn and hSn. A second objective was to determine …
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Regulation of Protective B cell Anti-Tumor Responses
… constitutively expressed by B cells. CD22, is a sialic acid-binding, Ig-like, lectin receptor (Siglec), and was found to significantly suppress antigen-specific antibody responses to mucins bearing sialylated TACAs. Inhibition of CD22 binding of sialic acids using monoclonal antibody blockade …
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Investigating the role of Sialoadhesin in Influenza A virus infection
Sialic acids (Sia) are abundantly found on the surface of mammalian cells, where they serve as a marker for “self” and mediate key cellular processes. Yet, Sia expression can be hijacked by certain “non-self” pathogens for immune evasion. Understanding the role of Sia receptors, and how they …
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NEU Insights and Development of Potential Therapeutics for Pulmonary Fibrosis
… desialylate glycoconjugates by cleaving terminal sialic acids from the glycoconjugates. Our lab previously found that a sialylated serum glycoprotein called serum amyloid P (SAP) inhibits fibrosis, and that sialidases attenuate SAP function. Mammals have four different sialidases, NEU1 – 4. In …
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Real Time PCR-Based Infectivity Assay and Characterization of Cell Surface Receptors for Turkey Hemorrhagic Enteritis Virus
… evidence of the utilization of cell surface sialic acids as attachment receptor for THEV. Destruction of surface carbohydrates and proteins on RP19 cells also reduced virus entry, indicating that these components are part of the THEV receptor. Using virus overlay protein blot assay, THEV was …
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Novel fusion proteins based on recombinant lectins for delivery of a cytotoxic peptide specifically to cancer cells
… an example which has strong binding affinity for sialic acids especially α2,6 sialic acid.In the present study, mistletoe lectin isoform I chain B (MLB) was targeted through conducting polymerase chain reaction (PCR) for cDNA which was reverse transcribed from the extracted V. album RNA. The …
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