Global ETD Search
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Showing 1 to 7 of 7 for “"SiMPull"”.
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Single molecule pull-down of viral RNA-protein complexes
… of mRNA. Recently, a single molecule pull-down (SiMPull) assay was developed to isolate and study single protein complexes directly from cell lysates. Unlike ensemble measurements, SiMPull is a powerful tool that allows detection of diverse proteins present in a single complex and quantitation of …
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Probing cellular protein complexes using single-molecule pull-down
… this technology single-molecule pull-down or SiMPull. In one reification biotinylated antibody against the protein of interest is immobilized on a flow chamber. The flow chambers are passivated using a polymer coating such that the cellular components do not bind, and the immobilized antibody …
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Redefining the specificity of phosphoinositide-binding by human PH domain-containing proteins
… has developed a single-molecule pull down (SiMPull) assay to probe protein-lipid binding. This method, called lipid-SiMPull, has high specificity and sensitivity while utilizing lipid vesicles of desired composition and full-length proteins expressed in whole cell lysates without the need …
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Development of single-molecules techniques to study native α-Synuclein aggregates
… non-specific signal. Single-molecule pull-down (SiMPull) is a recently developed technique that circumvents this issue, by immobilising the protein of interest on a passivated surface that is capable of blocking antibody binding. By combining SiMPull with the principles of two-colour coincidence …
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Single molecule pull down of double strand RNA binding proteins
… cells. We applied single molecule pull-down (SiMPull) assay to investigate the intensity of various dsRNA-dsRBP interactions. Our results demonstrate that despite the highly conserved dsRNA binding domains, the dsRBPs exhibit diverse substrate specificy. While TRBP and ADAR1 have a preference …
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Characterisation of neurodegenerative diseases derived protein aggregates using improved single-molecule pull-down
… an improved single-molecule pull-down (SiMPull) assay, which was used to characterise soluble α-Synuclein (αS) and Amyloid-β (Aβ) aggregates in serum from patients with PD and controls. Our findings reveal that the ratio of detected aggregates (αS/(Aβ+αS)) and morphological information …
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Mechanical manipulation and visualization of single biomolecules using fluorescence spectroscopy
… microscopy into a single-molecule pull-down (SiMPull) assay, to probe native macromolecular complexes. With this assay, we investigated the mechanism of disruption of complexes of mechanistic target of Rapamycin (mTOR), underlying neuronal signaling and -synuclein mediated neurodegeneration.