Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 16 of 16 for “"Shotgun Proteomics"”.
-
Development of Dendrimer-based Technologies for Phosphoproteomics and Glycoproteomics in Biomarker Discovery
… biochemical assays and mass spectrometry-based shotgun proteomics.
-
Potential for selection of new peanut genotypes with enhanced polyphenol antioxidant content
… of polyphenol biosynthesis in peanuts using the shotgun proteomics approach; 3) to screen for new phenolic compounds in a water-soluble peanut extract using LC-MS/MS and propose phenolic biosynthesis pathways, and 4) to investigate the feasibility of developing a simple nanoparticle-based test …
-
classCleaner: A Quantitative Method for Validating Peptide Identification in LC-MS/MS Workflows
… mass spectrometry (LC-MS/MS) shotgun proteomics infers the peptide sequence of each measurement, there is inherent uncertainty in the identity of each peptide and its originating protein. Removing misidentified peptides can improve the accuracy and power of downstream analyses …
-
Enhancing the Data, Information and Knowledge from Proteomics Experiments through Biomedical Informatics
… as an alternative to reductionism. In this work, shotgun proteomics is utilized along with systems thinking to quantify numerous proteins simultaneously and presents an unbiased, collective view of human health and disease. Following the capture of large amounts of proteomics data as mass spectra …
-
Proteomic insights into the marine obligate hydrocarbonoclastic bacteria (OHCB) during alkane degradation
… SK2, and Oleispira antarctica RB-8), LC-MS/MS shotgun proteomics was performed on cells growing on different hydrocarbons. T. oleivorans grew equally well on all substrates tested (n-C10-n-C32) as it utilised a subterminal alkane oxidation pathway whilst growing on long chain-alkanes, evidenced …
-
A proteomic investigation of the immune response of the South African abalone, Haliotis midae
… of H. midae remain obscure. High-throughput proteomics, a powerful tool to analyse global protein expression changes, can provide an integrated view of the immune system. Thus, this study aimed to elucidate the haemocyte proteome of H. midae and gain insight into regulatory molecular pathways …
-
Modulation of protein expression in Mycobacterium tuberculosis-stimulated TB- IRIS patient-derived PBMCs by omega n-3 polyunsaturated fatty acids
… Trypsin digestion, and investigated via MS-based shotgun proteomics. MaxQuant was used for protein identification and Perseus for statistical analysis. Stimulation with Mtb WCL shifted the secretome of healthy PBMCs towards an inflammatory state, and this was altered by treatment with EPA/DHA via …
-
Nuclear proteomics and transcription factor profiling in Chlamydomonas reinhardtii
… and performed nuclear proteome analysis using shotgun proteomics. This analysis permitted us to improve the genome annotation previously published and to discover conserved and enriched protein motifs among the nuclear proteins. In another approach, a quantitative RT-PCR platform was …
-
A Proteomics Approach to Investigate Uropathogenic Escherichia coli
… with a focus on ST127, using a quantitative proteomics approach. We employed a classical shotgun proteomics approach to analyse proteins extracted from multiple strains of UPEC following growth in various environments (Lysogeny broth, artificial urine medium and co-culture with uroepithelial …
-
A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… molecular and functional assays but also a shotgun proteomics based analysis of the global proteomes of differentiating mESCs versus EpiSCs. I demonstrate that, although mESCs at the beginning of their differentiation process share common characteristics with both naïve and primed PSCs, they …
-
Investigation of the effect of ocean acidification on the haemocyte proteome of the South African abalone, Haliotis midae
… species. This study employed a comparative shotgun proteomics approach using isobaric tagging for relative and absolute quantification (iTRAQ) coupled with LC-MS/MS to investigate the proteomic response of H. midae haemocytes to reduced pH conditions representative of future predictions of …
-
Using high-throughput proteomics to identify novel engineering targets for enhanced rAAV production in HEK293 cells
… expression and subcellular spatial proteomics were then applied to profile the response of HEK293-VP 2.0 cells to rAAV production. The effect of rAAV production on global protein abundance was determined by quantifying HEK293-VP 2.0 cell proteins in mock transfected and rAAV2-, …
-
IMPORTANCE OF SUPPLYING DAIRY COWS WITH ESSENTIAL FATTY ACIDS (EFA) AND CONJUGATED LINOLEIC ACIDS (CLA) DURING THE TRANSITION PERIOD ON METABOLISM AND HEALTH
… and lactation, respectively). An untargeted shotgun proteomics approach based on liquid chromatography coupled with tandem mass spectrometry was performed in liver biopsies and plasma samples collected at days -21, +1, +28, and +63 relative to calving. At each timepoint, differentially …
-
Mesenchymal stem cells as trophic mediators of neural differentiation
… of targeted transcriptional profiling and shotgun proteomics, and several putative candidate factors have been identified for further investigation.
-
Proteomic Analysis of Three Dimensional Organotypic Liver Models
… intercellular communication processes. Bottom-up shotgun proteomic analysis of the hepatic cells can lend insight into such processes. This technique employs liquid chromatography-tandem mass spectrometry (LC-MS/MS) for relative quantification of protein abundances by measuring intensities of …
-
Regulation of the carbon-concentrating mechanism in Chlamydomonas reinhardtii
… metabolic, as well as CCM-specific, changes. A shotgun proteomics approach (LC-MS/MS) was used to extend the analysis of the pyrenoid-less RBCS mutant proteome to the whole genome level. Approximately 10% of the total proteins detected using this method were identified as differentially …