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Showing 1 to 13 of 13 for “"Short linear motifs"”.

  1. Developing a Contextual Annotation Framework for Short Linear Motifs in Proteins

    Identifying and validating short linear motifs (SLiMs) is challenging due to their low sequence complexity and high prevalence across the proteome. Many false positives—sequences that match the pattern of the SLiM but are not involved in the biological functions typically associated with …

    mit Repository record for Developing a Contextual Annotation Framework for Short Linear Motifs in Proteins (opens in a new tab)

  2. How codon choice determines evolvability and evolutionary robustness in short linear motifs

    Short linear motifs, made up of 2-10 amino acids in linear sequence space, are a central component of cellular decision making through proteins. They form a modular system in cells where combinations of domains and motifs are used as basic functional building blocks through interactions. Functions …

    cambridge Repository record for How codon choice determines evolvability and evolutionary robustness in short linear motifs (opens in a new tab)

  3. Harnessing the Anaphase-Promoting Complex for Targeted Protein Degradation

    … APC/C substrates contain multiple copies of short linear motifs (SLiMs), termed degrons, that serve as APC/C-recognition sequences. My research approach involved systematically dissecting various combinations of degrons from natural APC/C substrates, primarily using cell-based degradation …

    cambridge Repository record for Harnessing the Anaphase-Promoting Complex for Targeted Protein Degradation (opens in a new tab)

  4. How short, degenerate motifs across the human proteome recognize the actin remodeling factor ENAH

    … interactomes are those that occur between short linear motifs (SLiMs) and structured domains. SLiMs are stretches of 3-10 residues found in intrinsically disordered regions of proteins. Given their degeneracy, short length, and weak affinities, it is not immediately clear how such low …

    mit Repository record for How short, degenerate motifs across the human proteome recognize the actin remodeling factor ENAH (opens in a new tab)

  5. TRP75-Mediated STAT3 Activation Promotes Anti-Apoptotic Signaling and Ehrlichia chaffeensis Infection

    … well-characterized TRP, TRP120, uses multiple short linear motifs (SLiMs) to interact with host proteins and co-opt signaling axes to inhibit apoptosis. Interestingly, other ehrlichial TRPs have been shown to interact with a diverse array of host proteins and harbor predicted SLiM sequences …

    utmb Repository record for TRP75-Mediated STAT3 Activation Promotes Anti-Apoptotic Signaling and Ehrlichia chaffeensis Infection (opens in a new tab)

  6. Determining the Molecular Underpinnings of Iron Homeostasis in Human Cells

    … these proteins. I found that NCOA4 bears two short linear motifs that each bind to GATE16 with weak affinity. However, these binding motifs are highly avid and, in concert, support high-affinity binding of NCOA4 to oligomerized GATE16. I further describe that ferritin degradation in cultured …

    mit Repository record for Determining the Molecular Underpinnings of Iron Homeostasis in Human Cells (opens in a new tab)

  7. Elaboration of the Homer1 Recognition Landscape Reveals Incomplete Divergence of Paralogous EVH1 Domains

    … identify interactions mediated by these Short Linear Motifs (SLiMs) is instrumental to unraveling the many biological processes regulated by this class of interactions. Characterizing the binding preferences of specific SLiM binding domains is key to both predicting interactions and …

    mit Repository record for Elaboration of the Homer1 Recognition Landscape Reveals Incomplete Divergence of Paralogous EVH1 Domains (opens in a new tab)

  8. Predicting motif mimicry in viruses

    … (DMI) between a globular domain and a short linear motif (SLiM) in its partner. Advances in high-throughput experimental techniques have been applied at large-scale in an attempt to characterise the interactomes of various organisms. However, the PPI networks identified by these …

    unsw Repository record for Predicting motif mimicry in viruses (opens in a new tab)

  9. Biochemical and functional characterization of human RNA binding proteins

    … RBPs bound an unexpectedly low diversity of RNA motifs, implying convergence of binding specificity toward a small set of RNA motifs characterized by low compositional complexity. Offsetting the low diversity of sequence motifs, extensive preferences for contextual features beyond short linear

    mit Repository record for Biochemical and functional characterization of human RNA binding proteins (opens in a new tab)

  10. The NTF2L Domain Is a Date Hub That Interacts with SLiMs to Regulate Assembly of Stress Granules

    … networks that drive biomolecular condensation. Short linear motifs (SLiMs) are the most abundant functional unit involved in protein-protein interactions (PPIs). The interactions between SLiMs and date hubs are crucial for various cellular processes. While SLiMs and date hubs are prevalent in …

    tenn-hsc Repository record for The NTF2L Domain Is a Date Hub That Interacts with SLiMs to Regulate Assembly of Stress Granules (opens in a new tab)

  11. Investigating how ubiquitin-mediated proteolysis of AURKA contributes to its activity in the cell cycle

    … outside mitosis, and examining the role of Short Linear motifs (SLiMs) within AURKA N-terminal domain in regulating its stability and activity. I show that the previously known D-box-like motif (R371xxL374) within C-terminal is not a functional degron. I also reveal that the A-box motif may …

    cambridge Repository record for Investigating how ubiquitin-mediated proteolysis of AURKA contributes to its activity in the cell cycle (opens in a new tab)

  12. Analysis of SMAUG1 protein motifs and interacting partners - linking regulation of phase separation to human disease

    … formation in cells. Using protein domain and short linear motif (SLiMs) deletion mutants, we demonstrated that SMAUG1 phase separation is driven by its structured ‘Smaug similarity region 1’ (SSR1) dimerization domain and a novel intrinsically disordered prion-like domain. In contrast, the …

    cork Repository record for Analysis of SMAUG1 protein motifs and interacting partners - linking regulation of phase separation to human disease (opens in a new tab)

  13. Organised Chaos: defining different degrees of intrinsic disorder by molecular dynamics methods

    … and signalling functions, such as p53 and short linear motifs (SLiMs)-containing regions. Trying to understand the relationships between structure and functions in these highly flexible and dynamic systems is rather challenging, because their intrinsic flexibility makes them extremely …

    maynooth Repository record for Organised Chaos: defining different degrees of intrinsic disorder by molecular dynamics methods (opens in a new tab)