Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"Shigella flexneri"”.
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Enterobacterial Common Antigen Biosynthesis in Shigella flexneri
… thesis was to investigate ECA biosynthesis in Shigella flexneri, with particular focus on WzyE, the Wzy protein from ECA biosynthesis. For the first time, a WzyE protein was directly investigated and the data showed that WzyE is uncharacteristic of other Wzy proteins; showing a high amount of …
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Polarity and secretion of Shigella flexneri IcsA: a classical autotransporter
… virulence factor for the enteropathogen Shigella flexneri as it provides adherence properties and allows intra- and intercellular spreading in the colonic mucosa. IcsA is an outer membrane surface protein that specifically hijacks host-cell actin recruiting and polymerising complexes …
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The Biogenesis and Homeostasis of the Shigella flexneri Cell Envelope
Shigella flexneri is a significant cause of gastroenteric disease in the developing world, predominantly devastating the paediatric age group. This bacterial pathogen synthesises a plethora of polysaccharide-based complexes on its cell surface, such as lipopolysaccharide (LPS), that facilitate host …
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Characterizing induced gene expression in Shigella flexneri following bile salt exposure
The Shigella species cause millions of cases of watery or bloody diarrhea each year in developing countries, with children under the age of five years most vulnerable to infection. Emerging strains of multidrug resistant Shigella emphasize the need for a comprehensive and cost-efficient vaccine; …
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An immunological comparison of animal-grown and broth-grown shigella flexneri types lb and 3
… following recovery from this disease. However, shigella vaccines made from in vitro-grown organisms did not increase resistance to dysentery. The purpose of the present investigation was to determine whether or not Shigella flexneri produced antigens in the in vivo environment formed by …
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Identification of host factors required for cytosol entry by Shigella flexneri: Results of a genome-wide CRISPR/Cas9 screen
The Gram-negative bacterial pathogen *Shigella flexneri* remains a globally significant cause of morbidity and mortality. *Shigella* induces its uptake into colonic epithelial cells via a short-lived vacuole, and lyses the vacuole membrane to gain entry to the host cytosol. As a professional …
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Characterization of novel virulence factors of Shigella flexneri and development of Caenorhabditis elegans as an animal model for Shigella infection
The Gram-negative bacterium Shigella flexneri is the causative agent of Shigellosis, also known as bacillary dysentery, responsible for 5-15% of the global burden of diarrhoeal disease. Despite over 50 years of Shigellosis research, the search for a commercially viable vaccine is ongoing. A …
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Characterization of novel virulence factors of Shigella flexneri and development of Caenorhabditis elegans as an animal model for Shigella infection
The Gram-negative bacterium Shigella flexneri is the causative agent of Shigellosis, also known as bacillary dysentery, responsible for 5-15% of the global burden of diarrhoeal disease. Despite over 50 years of Shigellosis research, the search for a commercially viable vaccine is ongoing. A …
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Modelling and validation of bacterial O-antigen conformations: ring puckering in Shigella flexneri 7a and 7b O-antigens as a case study
… investigating the conformation and dynamics of Shigella flexneri O-antigens. S. flexneri is a leading cause of diarrhoeal related diseases, especially in Sub-Saharan Africa and Southeast Asia. There are over 30 S. flexneri serotypes and, with the exception of serotype 6, all share a common …
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Towards the Synthesis of the Unusual Monosaccharides Found in the Shigella sonnei O-Antigen and Analysis of Shigella flexneri 2a Glycoconjugate Vaccine Samples
… by the Gram-negative bacteria belonging to the Shigella species. Despite over 60 years of vaccine research, no licensed vaccine to prevent shigellosis is commercially available. Bioconjugate vaccines based on the O-antigen against various Shigella serotypes are under development. Shigella sonnei …
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Biochemical Characterization of IpaH E3 Ubiquitin Ligase Effector Proteins and Their Host Substrates
Shigella flexneri is a gram negative pathogen that utilizes its type 3 secretion system (T3SS) to inject effector proteins in the cytoplasm of host cells to manipulate host cells processes. T3SS effectors are able to post translationally modify host proteins to reprogram intracellular signaling …
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Interaction of Salmonella typhimurium and Listeria monocytogenes with the murine host
… these pathogens, eg Salmonella typhimurium and Shigella flexneri, use M cells, found only within the follicle associated epithelium (FAE) that overlies Peyer’s patches and other lymphoid follicles, to enter the host. This study is primarily an investigation into the interaction of S. typhimurium …
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Functional analysis of Shigella encoded IpaH E3 ubiquitin ligases in cell-autonomous immunity
Shigella flexneri is a highly adapted pathogen that invades the host cytosol and causes bacillary dysentery. Shigella has evolved powerful countermeasures to disarm host defense mechanisms; amongst them a family of twelve bacterial E3 ubiquitin ligases (IpaH) that are structurally unrelated to …
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The screening of medicinal plants traditionally used to treat diarrhoea, in Ongoye area, KwaZulu Natal.
… aureus. Escherichia coli, Salmonella spp, Shigella flexneri and Shigella sonnei. The antibacterial activities were determined by disk-diffusion, agar-well diffusion, minimum inhibitory concentrations (MIC) and bio-autographic methods. The plant extracts were screened for the following …
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Design of a high-affinity carbohydrate binding protein
… wie Escherichia coli, Salmonella spp. oder Shigella flexneri durch Bakteriophagen der Familie der Podoviridae erfolgt über die Bindung eines sogenannten tailspike proteins des Bakteriophagen an das aus Kohlenhydraten-bestehende O-Antigen des Lipopolysaccharids von Gram-negativen Bakterien. …
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Bacterial Manipulation of Actin Dynamics via p21 Activated Kinases and Cofilin
… ability to enter non-phagocytic cells, and Shigella flexneri to assemble actin ‘comet tails’ for intracellular propulsion and bacterial dissemination. This work sought to investigate the role that cofilin and PAK1, as well as its upstream and downstream regulators has in actin remodelling, …
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An ethnobotanical and antidiarrhoeal investigation of plants used traditionally in the Maputaland area homesteads
… activity (mean MIC value of 0.04 mg/ml against Shigella flexneri). Many other plant species showed noteworthy activities against different pathogens. More than 80% of plant species screened were active against at least one out of seven of the diarrhoeal pathogens. Proteus vulgaris showed overall …
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