Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"Shieldin"”.
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53BP1/Shieldin Counteract DSB Resection Through Fill-In Synthesis
… 53BP1 and its downstream effectors RIF1 and the shieldin complex limit the generation of long 3' overhangs at DSBs by recruiting CST/Polα to counteract resection, challenging the long-standing model of a block to resection by this pathway. Like loss of 53BP1/RIF1/shieldin, disruption of …
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Interrogating DNA replication stress and DNA damage responses via phenotypic and functional CRISPR/Cas9 screens
… by the inactivation of genes encoding the SHIELDIN complex, which causes resistance of BRCA1-mutant cells to PARP inhibitors. Lastly, I conceptualised and performed functional genome-wide CRISPR/Cas9 screens, utilising the chromatinisation of RPA as a read-out for DNA repair processes, to …
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53BP1 and double-strand break repair pathway choice in cancer
… other resistance mechanisms, we identified the shieldin complex (SHLD1/C20orf196 and SHLD2/FAM35A) that when lost suppressed PARP inhibitor sensitivity in BRCA1-deficient cancer cells. We demonstrate that SHLD1/2 represent the terminal end-effectors of the 53BP1-RIF1-MAD2L2 axis, supporting NHEJ …
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Development of Novel Technologies to Investigate DNA Double-Strand Break Repair Uncovers a Role for the ATM Kinase in Error-Free NHEJ with Implications for Neurodegenerative Diseases
… between 53BP1 and RIF1 independently of the Shieldin and CST complexes, which we propose serves to physically hold DSB ends together in a redundant manner with the core NHEJ-mediated end synapsis machinery. Second, we propose that ATM promotes EF-NHEJ via promoting R-loop resolution by both …