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Showing 1 to 20 of 30 for “"Shewanella oneidensis"”.
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Bioreduction of Hematite Nanoparticles by Shewanella oneidensis MR-1
… mechanism may be simultaneously employed by Shewanella oneidensis MR-1 during solid-phase metal reduction. This conclusion is supported by analysis of the bioreduction kinetics of hematite nanoparticles coupled with microscopic investigations of cell-mineral interactions. The reduction …
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Characterisation of OmcA From Shewanella oneidensis MR-1: Biophysical and Mineral Reduction Properties
Several!Shewanella(spp!are!versatile!in!the!respiratory!substrates!they!use.! A! novel! set! of! respiratory! substrates! implicated! are! insoluble! Fe(III)! and! Mn(III,IV)! oxides.! OmcA! from! Shewanella( oneidensis( MR?1! plays! a! role! in! the! …
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Biofilm formation by Shewanella oneidensis MR-1 towards fundamental understanding of anode respiring communities
… organism associated with microbial fuel cells is Shewanella oneidensis, containing multiple EET mechanisms and an apparent inability to form multi-layered biofilms in anaerobic culturing, i.e. microbial fuel cell operating conditions. In contrast to G. sulfurreducens, S. oneidensis biofilm …
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Structural and spectroscopic studies on the porin-cytochrome complex from Shewanella oneidensis MR-1
… process"of"dissimilatory"metal"reduction"(DMR)"in"Shewanella(oneidensis." "" The"properties"of" the"decaheme"protein"MtrA"as"well"as"a" truncated"version"of" this"protein" were" investigated" using" analytical" ultracentrifugation" (AUC)," small" angle" X!ray" scattering" (SAXS)" and" …
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Shewanella oneidensis MR-1 cell-to-cell signaling and its influences on biogeochemical processes
… sensing (cell-to-cell signaling) abilities of Shewanella oneidensis MR-1, a Gram-negative bacterium well known for its ability to use geologic substrates, such as Fe and Mn oxides, for respiratory purposes. Overall our results show that S. oneidensis cannot utilize either an acyl-homoserine …
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Transcriptional Regulation of Shewanella oneidensis Using Native TMAO-Inducible Promoters in a Plasmid-Free System
Shewanella species are well known for their ability to respire a multitude of compounds, including insoluble extracellular metals. Shewanella oneidensis MR-1 has emerged as a model organism for understanding the process of extracellular electron transport (EET), in which cells transfer …
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Electrical current generation by wild type and mutant Shewanella strains
The genus Shewanella has been reported to have the capacity to couple the transfer of electrons to insoluble metal oxides and solid carbon electrodes with cellular growth. While this process may be useful as an energy generation strategy or biotechnological tool, the electron transfer pathway by …
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Microbial Biofilm Development and Electron Transfer in Electrochemical Systems
… particularly, for the facultative anaerobe Shewanella oneidensis MR-1. These systems consist of microbial fuel cells (MFCs) whose anode component will be evaluated with regards to performance, electrical current generation, and microbial coverage and biofilm development. A better …
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Implementation of nano-liquid chromatography hyphenated to tandem mass spectrometry for protein identification in gel and non-gel based proteomics
… of the dissimilatory iron-reducing bacterium Shewanella oneidensis MR-1 grown on ferric oxide (Chapter V) and the effect of a short-term heat shock on the plant barley (Chapter VI). Part III deals with the development of alternative strategies for the analysis of membrane proteins. A special …
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Development of Integrated Photobioelectrochemical System (IPB): Processes, Modeling and Applications
… application of stable isotope probing to study Shewanella oneidensis MR-1 in the MFCs. Chapters 6 to 8 describe the application of models to optimize MFC and IPB system performance. Chapter 9 describes the strategy improvement for the algal harvesting in IPB. Chapter 10 describes the application …
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Engineering outer-membrane electron transfer proteins for modular biotic-abiotic interfacing with living cells
The electrogenic bacterium Shewanella oneidensis utilises the MtrCAB protein complex to transfer electrons across the outer membrane, forming a biotic-abiotic interface that can be harnessed for energy generation, biosensing, microbial electrosynthesis and cell signalling. Within this complex, the …
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The Effects of Iron Oxidation State on Surface Chemistry and Structure of Iron-Bearing Smectites: Cation Fixation and Selectivity, Structural Iron Environment, and Dissolution
… was reduced to similar levels as by either by Shewanella oneidensis or by pH-buffered dithionite. The dissolution of smectites by bacterial reduction without organic acids was nonstoichiometric and insignificant to moderate levels. In general, the changes in smectite structure during redox …
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Interfacial and long-range electron transfer at the mineral-microbe interface
… cytochromes from the metal-reducing bacterium Shewanella oneidensis were prepared on Au(111) surfaces. Recombinant tetracysteine sequences were added to two outermembrane decaheme cytochromes (OmcA and MtrC) from S. oneidensis MR-1 to ensure chemical immobilization on Au(111). STM images of the …
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Sustainable photocatalysis using carbon-based materials through interfacial and solvent engineering
… to develop an efficient biohybrid system with Shewanella oneidensis MR-1 for ethanol and H2 co-production from glycerol upcycling, enhancing extracellular charge transfer between bacteria and abiotic nanomaterials. Finally, an air-tolerant solar reforming system for biomass and plastics was …
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Electrical-phenotyping of the bacterial envelope using microfluidics
… activities, including Geobacter sulfurreducens, Shewanella oneidensis, and Escherichia coli heterologously expressing Shewanella EET pathways. We also applied 3DiDEP to achieve rapid quantification of LPS, the major component and virulence determinant in Gram-negative bacterial outer membrane. We …
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Mineral-Microbe Interactions Probed in Force, Energy, and Distance Nanospace
… the transfer of electrons from biomolecules on Shewanella oneidensis to Fe(III) in the structure of goethite. Force measurements with picoNewton resolution were made in aqueous solution under aerobic and anaerobic conditions. Energy values (in attoJoules) derived from these measurements show …
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Development and Application of Fluxomics Tools for Analyzing Metabolisms in Non-Model Microorganisms
… analysis to decipher the dynamic metabolisms of Shewanella oneidensis MR-1. The sub-optimal metabolism and the time-dependent metabolic fluxes were profiled in a genome-scale metabolic network. A web-based platform was constructed for high-throughput metabolic model drafting to bridge the gap …
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Investigation of Rhenium's Biogeochemistry
… metallireducens<italic> GS-15, <italic>Shewanella oneidensis<italic> €MR-1, <italic>Desulfovibrio desulfuricans<italic> subsp. <italic>desulfuricans<italic> and <italic>Desulfovibrio desulfuricans<italic> ND132. I concluded that neither direct nor short-term indirect microbial …
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Characterisation of cytochrome c MtoD from the iron-oxidising autotroph sideroxydans lithotrophicus ES-1
… MtrCAB complex from iron-reducing bacterium Shewanella oneidensis, albeit in reverse. MtoD is predicted to be a monoheme class I cytochrome c that is proposed to transfer electrons from MtoA to the predicted inner membrane quinone reductase CymAES-1 (Shi et al. 2012). Very little is …
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Bioremediation of petroleum hydrocarbons using microbial fuel cells
… cells (MFCs) using different inoculum types - Shewanella oneidensis MR1 14063, Pseudomonas aeruginosa NCTC 10662, mixed cultures and their combinations thereof. All the inocula showed high potentials for phenanthrene and benzene degradation in liquid systems with a minimum degradation …
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