Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"SgrS"”.
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Regulation of the Mannose PTS Operon by the small RNA SgrS
… expression of multiple genes. The sRNA SgrS is expressed in response to glucose-phosphate stress, a condition associated with disruption of glycolytic flux and accumulation of sugar-phosphates. SgrS has been shown to stimulate degradation of the ptsG mRNA, encoding the major glucose …
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Target regulation and prioritization by the small RNA SgrS in Escherichia coli
… Likewise, the Escherichia coli small RNA SgrS controls a response to metabolic stress that occurs upon cytoplasmic accumulation of glucose-phosphates due to mutations in glycolysis (e.g. in pgi) or when cells take up glucose-analogs α-methyl-D-glucoside (αMG) and 2-deoxyglucoside (2DG). …
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Mechanistic and physiological insights into post-transcriptional regulation by small RNAs SgrS and DicF
… multitude of clever mechanisms that two sRNAs, SgrS and DicF, utilize to regulate gene expression, and the physiological consequences of such regulation. The SgrS sRNA participates in a response to a growth inhibitory stress condition called sugar-phosphate stress caused by the toxic …
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Sweet relief: regulating the response to glucose phosphate stress by the small RNA SgrS & Co.
… by producing a small RNA (sRNA) regulator, SgrS, which represses translation of sugar transporter mRNAs (ptsG and manXYZ) and enhances translation of a sugar phosphatase mRNA (yigL). Despite a molecular understanding of individual target regulation by SgrS, previously little was known about …
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Developing high-resolution methods to study DNA and RNA dynamics
… gene expression regulation. Our research target, sgrS, is a bacterial small RNA that regulates several target genes at post-transcriptional level in response to sugar-phosphate stress. It is known that sgrS anneals target transcripts via basepairing interaction with the guide of Hfq protein. …
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An E. coli small RNA inhibits translation initiation from a distance
… biology. In the targetome of an E. coli sRNA SgrS, manXYZ is a non-canonical target where SgrS base pairs at two distinct sites that are far from the SD regions of manX and manY, yet translation of these two cistrons are repressed by SgrS. We found that manX translation is controlled by a …
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Flow mechanisms and numerical simulation of gas production from shale reservoirs
… hydrocarbon formations, shale gas reservoirs (SGRs) present numerous challenges to modeling and understanding due to complex pore structure, ultra-low permeability, and multiple transport mechanisms.</p><p>In this study, the deviation against conventional gas flow have been detected in the lab …
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Molecular analysis of small RNA and small protein regulation of Escherichia coli stress responses
… and the protein product of a dual-function sRNA, SgrS in Eschericha coli. The sRNA SgrS encodes the small 43-amino acid protein SgrT. Both molecules are expressed during glucose-phosphate stress - a bacteriostatic condition in which phosphosugars accumulate in the cell either because of mutations …
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A combinatorial approach for improved target prediction, validation, and characterization of small RNAs in Escherichia coli
… train the pipeline with well-characterized sRNAs SgrS and RyhB, with SPOT achieving > 75% sensitivity (Correctly Predicted Targets/Total Known targets). We employed a combinatorial approach combining both SPOT and experimental evidence to identify potentially overlooked targets for the …
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The contribution of viral and host cell factors to replication of the hepatitis C virus RNA genome
… at these sites. This result was confirmed using SGRs harbouring a photoactivatable derivative of GFP (PAGFP). Utilising plasmid-encoded HCV polyproteins, it was apparent that the targeting of NS5A to these structures was dependent on NS4B. Removal of the NS4B coding region resulted in a diffuse, …
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Understanding The Carotenoid Biosynthesis Pathway in Orange Actinidia Species
… (a rate-limiting step in most plant species) and SGRs in Nicotiana tabacum leaves resulted in a significant increase in carotenoids and de-greening, respectively. The rapid de-greening accompanied by β-carotene accumulation can also depend on the transcriptional regulation of the pathway and …