Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Serum deprivation"”.
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Protein kinase C modulates mitogenic signalling by epidermal growth factor in T51B rat liver cells.
… be made proliferatively quiescent upon prolonged serum deprivation, which results in their growth-arrest at the G1 phase of the cell cycle. Addition of EGF (1.5$\eta$M) or, re-addition of serum (10% BCS) to the conditioned medium initiates DNA synthesis, within 8 hours, which reaches a maximum by …
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Intracellular sphingosine kinase activity as a regulator of endothelial cell inflammatory and angiogenic potential.
… cell survival under the stressful conditions of serum deprivation and loss of attachment with extracellular matrix, which was associated with suppression of apoptotic mechanisms. Raised SK activity also stimulated cell migration and cellular remodeling, additional measures of angiogenesis. …
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Expression profile of multidrug resistance genes and proteins in cancerous and stem cells
… using chemosensitivity assay. Meanwhile, a serum deprivation method was established in this study to enrich and select CD 133+ CSCs in a glioma (GOS-3) cell line. As a secondary focus of this project, the possibility of immortalisation enzyme hTERT being a discriminative masker between …
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Investigating the function of PINK1 in cancer development and pathogenesis
… in proliferative phases upon removal of serum. The ability of cells to progress through mitosis conferred by PINK1 expression was independent of its kinase activity, while the cell cycle exit following serum withdrawal was kinase dependent. Investigations into the mechanism through which …
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Processing of Alzheimer's amyloid precursor protein in cultured cells.
… in secreted APP. Both cell types were also serum deprived, which resulted in little effect on protein production in NT2 stem cells. However, the neuronal cells showed a small increase in intracellular, KPI-containing APP and in HSP. A reduction in overall APP secretion, and cessation of KPI …
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CD30: A Novel Receptor to Induce Apoptosis in Human Blood Eosinophils
… cytometry. Spontaneous apoptosis was induced by serum deprivation, and survival was conferred by incubating cells with 10% FBS and 15 ng/ml IL-5. CD30 surface expression was upregulated in eosinophils incubated for 24 hours as compared to freshly isolated eosinophils, and both CD30 expression and …
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The role of nerve growth factor and p75 neurotrophin receptor in recovery from liver fibrosis
… protects human hepatic myofibroblasts from serum-deprivation and cycloheximide-induced apoptosis. To define the dominant effect of p75 neurotrophin receptor-mediated events in experimental liver fibrosis I have used a mouse lacking the p75 neurotrophin receptor ligand-binding domain but …
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Modulation of DNA repair pathway after CRISPR/Cas9 mediated Double Stranded Break
… chemical treatment and the frequency of NHEJ by serum starvation. CRISPR/Cas9 systems targeting RAG2 gene and donor DNA to replace endogenous RAG2 were transfected into porcine fetal fibroblast (PFF) cells and the cells were treated with various chemicals that were known to inhibit NHEJ or …
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The Role of Sphingosine Kinase 2 in Cell Growth and Apoptosis
… caspase-dependent apoptosis in response to serum deprivation, independently of S1P receptors. Sequence analysis revealed that SphK2 contains a 9 amino acid motif similar to that present in BH3-only proteins. Studies showed that the BH3-only domain, catalytic activity, endoplasmic reticulum …
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Role of BTG2/TIS21/PC3 in differentiation of myeloid leukemia cells and its regulation under stress conditions
… in response to cell stress challenges such as serum deprivation and oxidative stress i.e. H2O2, or doxorubicin treatments. We observed close interrelation between generation of reactive oxygen species (ROS), enhanced IκBα degradation, nuclear translocation of NF-κB(p65/RelA) and the significant …
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Stemness status in differentiated and undifferentiated glioma cells
… were investigated in control, hypoxic and serum-deprived U87-MG cells in order to shed light on the influence the micro-environment has in generating stem cells. Hypoxia offered a rapid state of undifferentiation as compared to serum deprivation by expressing a basal level of CD133 protein, …
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Elucidating the regulatory mechanisms of pterygium
… study we investigated the effects of reduced serum (to mimic a suppressed blood supply) and treatment with transforming growth factor β on cell signalling and function of pterygial derived fibroblasts. Methods: Pure fibroblast cultures were established from cell outgrowths of pterygial tissue. …
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The Role of a Membrane Androgen Receptron in the Brain
… and an increase in apoptotic cell death during serum deprivation. Here, I hypothesize that activation of a mAR in cortical astrocytes, suppresses the ERK and Akt signaling pathways and increases cell death in the presence of a metabolic and oxidative stressor. In this study, we found that glia …
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Trasduzione del segnale e poliamine nell'apoptosi di cellule cardiache
… by several stimuli including hypoxia, serum withdrawal or combination of both. Several lines of research suggest that neurohormonal mechanisms play a central role in the progression of heart failure. In particular, excessive activation of the sympathetic nervous system or the …
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The role of sphingosine kinases and SKAM1 in cutaneous wound healing
… overexpressing SKAM1 were resistant to serum deprivation-induced apoptosis. We also generated SKAM1 transgenic mice, where SKAM1 was ubiquitously expressed, to study the role of this protein in wound healing in vivo. We found no observable phenotypical difference between SKAM1 transgenic …
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Aktivierung von Caspasen in AKR-2B Mausfibroblasten
… Zellinie dar, in der durch Entzug des Serums der Zelltod induziert wird. Während des Zelltods sterben innerhalb von 6h etwa 50 Prozent einer dichtearretierten Kultur. Die überlebenden Zellen bleiben von diesem Mangelzustand für mindestens weitere 48h unbeeinflußt, benötigen aber zum …
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The Role of Acidic Fibroblast Growth Factor and Fibroblast Growth Factor Receptor 4 In High Grade Serous Carcinoma
… The effect of FGF1 on these cell lines after serum starvation was quantified for <em>in vitro</em> cellular proliferation, migration/invasion, chemoresistance and survival utilizing a combination of commercially available colorimetric, fluorometric and electrical impedance assays. FGFR4 …
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The role of topoisomerase II in amsacrine resistance
… induced into a G1 state by temperature arrest or serum deprivation, Human Jurkat cells selected for resistance to amsacrine and displaying cross-resistance to other topoisomerase II-targeted drugs also exhibited significantly reduced PDC enhancing activity. These results suggested a contributing …
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