Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 117 for “"Serine Protease"”.
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Caldolase: an alkaline serine protease from Thermus strain TOK₃
… of extreme thermophiles producing extracellular proteases, and the biochemical characterisation of a stable, chelator-insensitive protease. Two plate assay systems were developed for the initial screening of proteases. The first involved the incorporation of various protease inhibitors …
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The Role of Serine Protease Inhibitors in Regulating Hepatotoxicity During Viral Infection
… 9 (PI-9/ serpinB9) and the murine orthologue, serine proteinase inhibitor 6 (SPI-6/ serpinb9) are members of a family of intracellular serine proteinase inhibitors (serpins). PI-9 and SPI-6 expression in immune-privileged cells, antigen-presenting cells, and cytotoxic T cells protects these …
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Activity of Analogs of Anticancer Drugs on the Serine Protease Enzymes Subtilisin and Chymotrypsin
… activity. Chymotrypsin and subtilisin are serine proteases that have a histidine residue in the active site. We are investigating the inhibition of the digestive enzymes chymotrypsin and subtilisin by analogs of the anticancer drug cisplatin and trying to discern trends in the inhibition as …
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Characterization Of A Novel Interactor/substrate For The Pro-apoptotic Serine Protease Omi/htra2
OmiHtrA2 is a highly conserved mammalian serine protease that belongs to the HtrA family of proteins. Omi shares homology with the bacterially expressed heat shock protease HtrA, which functions as a protease at higher temperatures and a chaperone at lower temperatures. Additionally, Omi shares …
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INHIBITION OF THE SERINE PROTEASE CHYMOTRYPSIN BY A SILANEDIOL PEPTIDE MIMIC: ASYMMETRIC SYNTHESIS AND INHIBITION STUDIES
… outstanding inhibition of metallo and aspartic proteases. This research describes the synthesis and inhibition of the serine protease α- chymotrypsin by silanediol structures and the development of an efficient method to install the stereochemistry at the P1’ position of the inhibitor. In the …
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Novel Protein-protein Interactions Regulate The Proteolytic Activity Of The Pro- Apoptotic Serine Protease, Omi/htra2
Omi/HtrA2 is a mitochondrial serine protease with high homology to the bacterial HtrA proteins. Omi promotes caspase-dependent apoptosis by binding and degrading IAPs-inhibitor of apoptosis proteins. Omi can also induce caspase-independent apoptosis but the actual mechanism is still unknown. IAP's …
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Purification of Two Myosin -Degrading Proteases From Porcine Skeletal Muscle
… the major skeletal muscle protein. However, the protease system responsible for myosin degradation in vivo has not yet been clearly described. Two myosin degrading proteases, a serine protease and a cysteine protease that might have some function in myosin degradation, were isolated from porcine …
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Molecular characterisation of the vibrio midae SY9 extra cellular alkaline serine protease and its role in the previously observed probiotic effect on the growth of Haliotis midae
… predominant V. midae SY9 extracellular alkaline protease, VmproA, was investigated in an attempt to determine the role of VmproA in the growth enhancing probiotic effect observed by Macey and Coyne (2005) for abalone fed V. midae SY9 supplemented feed. The V. midae SY9 gene, vmproA, encoding the …
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Isolation and characterization of starch, starch biosynthetic genes and protease inhibitors from Marama beab (Tylosema Esculentum)
… marama starch biosynthesis genes and detect serine protease inhibitor activities in green and mature marama seeds. The total starch content of marama bean tubers was determined by amyloglucosidase/α-amylase enzymatic digestion and amylose content by Concanavalin A precipitation. A …
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Evaluating the Co-commensal Lifestyles of Staphylococcus aureus and Staphylococcus epidermidis
… is a ubiquitous skin commensal, and produces a serine protease Esp that can eradicate nasal carriage of the opportunistic pathogen, Staphylococcus aureus. We evaluated the ability of S. epidermidis and S. aureus to express serine protease and compete with one another in response to acidic pH and …
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The synthesis and evaluation of halo and protio enol lactone Pro-Val pseudodipeptides as potential inhibitors of human leukocyte elastase
Mechanism-based inhibitors of the serine protease $\alpha$-chymotrypsin have previously been prepared from aryl-substituted halo and protio enol lactones. In this study, the enantiomerically pure disubstituted protio and halo enol lactones 11a-c and 12a-b were prepared as potential inhibitors of …
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Molecular biology studies on the extracellular serine proteases of Vibrio alginolyticus
… bacterium that produces several extracellular serine proteases and a collagenase during the stationary growth phase. The aim of this study was to investigate alkaline serine protease production by this organism, and to attempt the cloning and expression of a V.alginolyticus protease gene in …
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Genetic Analysis Of Rhoa Signaling During Epithelial Morphogenesis In Drosophila
… (Sb-sbd) locus, a transmembrane serine protease. Mutations in the Sb-sbd gene interact genetically with the members of the RhoA signaling pathway, however the nature of the relationship between Sb-sbd serine protease activity and RhoA signaling is not understood. To identify …
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Ester bond formation in bacterial adhesins
… forms spontaneously, in this case utilising a serine protease-like mechanism and a recognisable catalytic triad. However, unlike the serine protease mechanism where a water molecule attacks and hydrolyses the acyl intermediate to regenerate the active site, the ester bond in Cpe0147 is stable …
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Serpin polymers enforce molecular filtration in the endoplasmic reticulum
… and cause disease. Alpha1-antitrypsin is the serine protease inhibitor (serpin) that is secreted mainly by hepatocytes and acts in lungs where its major function is to inhibit neutrophil elastase. Neuroserpin is a serine protease inhibitor expressed in the nervous system. Mutations in these …
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The penetration protease of the cercariae of Schistosoma mansoni
This thesis is concerned with a study of the proteases released by the cercariae of Schistosoma mansoni while penetrating mammalian skin. The proteases present in secretions collected from the preacetabular glands of cercariae were shown to be active against ¹²⁵I-labelled fibrin but not against …
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Functional characterisation of the lipid raft protein stomatin
… Prokaryotic stomatin exists in an operon with a serine protease, suggesting a functional link between the two. Using a reporter gene construct approach the potential for mammalian stomatin to be proteolytically processed was investigated. Stomatin was found to be proteolytically processed in the …
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