Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 118 for “"Sequencing analysis"”.
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Next-generation sequencing analysis and RNA editing in human brain and glioma
RNA sequencing (RNA-seq) is one of the most common technologies in use today for the analysis of gene expression and transcriptome variation in biological samples (Zhong Wang et al. 2009). As of 2011, the NCBI Sequence Read Archive (SRA) surpassed 100 terabases of sequence data, comprising nearly …
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Insights into the genomic histories of diverse human populations using whole-genome sequencing analysis.
… de novo assembly of 25 genomes using linked-read sequencing, I discovered 1643 breakpoint-resolved unique insertions, in aggregate accounting for 1.9 Mb of sequence absent from the GRCh38 reference genome, highlighting the limitation of a single human reference genome. In the second project I …
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Evaluation of Computational and Experimental Parameters in RNA Bisulfite Sequencing Analysis and Applications in Brain Development Studies
… of m5C. In this work, we utilize RNA bisulfite sequencing as a transcriptome-wide approach to understand the computational and chemical parameters needed to optimize m5C discovery in the mitochondria and the developing brain. In Chapter 1, we systematically evaluate four preparation conditions …
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Construction of bovine adenovirus type 3 E1 and E3, substitution plasmids and the sequencing analysis of DNA pol and pTP /
… of the virus genome accessible. Therefore, sequencing of the BAV3 genome from 1 1 .7% to 30.8% was carried out. Analysis of the determined sequences revealed the primary structure of important viral gene products coded by E2 including BAV3 DNA pol and precursor to terminal protein. …
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Investigation of Sensory Cell-Specific Changes in Gene Expression in Microrna-183 Family Knockout Mouse Inner Ear From Bulk RNA Sequencing Analysis
… inner ear, our research team conducted bulk RNA sequencing to compare newborn mouse inner ear tissues from wild-type versus miR-183 family knockout (KO) mice. Bulk RNA sequencing revealed numerous gene expression changes in the cochlear sensory epithelium and spiral ganglion due to miR-183 family …
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ΜΕΛΕΤΗ ΤΩΝ ΑΠΛΟΤΥΠΩΝ ΚΑΙ ΜΕΤΑΛΛΑΓΩΝ ΤΗΣ Β-ΘΑΛΑΣΣΑΙΜΙΑΣ ΣΤΗΝ ΕΛΛΑΔΑ
… ALIGONUCLEOTIDES. A NEW MUTATION WAS DETECTED BY SEQUENCING ANALYSIS.
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Association of Lamivudine-Resistant Mutational Patterns with the Antiviral Effect of Adefovir in Patients with Chronic Hepatitis B
… by restriction fragment mass polymorphism (RFMP) Analysis 5 D. Sequencing analysis 6 E. Definition 6 F. Statistical analysis 7 III.RESULTS 8 A. Virologic and biochemical response to adefovir 8 B. Antiviral efficacy of Adefovir between the HBeAg-positive and -negative Groups 8 C. Baseline hepatitis …
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Modifikation der enzymatischen Mutationsdetektionsmethode (EMD) zur Identifikation von Punktmutationen im PMP22-Gen nach DNA-Isolierung aus Nerv, Muskel oder Blut
… pathological mechanisms, molecular genetic analysis is very important. In this study, DNA form 59 patients was examined for point mutations in the PMP22-Gene, which codes for a transmembrane protein in the myelin sheath. After DNA-extraction, the four coding exons of the gene were amplified …
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An in vivo study of novel genetic modifiers in autosomal recessive polycystic kidney disease (ARPKD)
… Muc1, Tacstd2 and Epor, were identified via RNA-sequencing analysis with metabolic pathways such as oxidative phosphorylation appearing dysregulated. In the liver, Pkhd1T37M/T37M mice presented progressive liver fibrosis and increased canonical Wnt signalling. Excitingly, for the first time, we …
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Novel mutations of the A mating-type idiomorph in Neurospora crassa
… heterokaryon compatible A mutants, mutational analysiswas performed on mating-type A strains using 6-N-hydroxylaminopurine (H.A.P.), achemical mutagen that produces GC-*AT base pair substitutions, in addition to UV. All of the mutants isolated with UV were heterokaryon compatible and sterile. …
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A study of the metK gene encoding S-adenosylmethionine synthetase in Escherichia coli K-12
… under certain growth conditions. Through DNA sequencing analysis and primer extension analysis, I showed that this mutant carried an A[arrow right]G transition in the -10 region of the metK promoter. In this work, the initiation site for metK transcription was determined by primer extension. …
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Microbial communities as predictors of outcomes in industrial and clinical applications
… of diversity. In this thesis, I use 16S rRNA sequencing analysis to measure community composition across time series to predict outcomes for three applications: bioreactor function; a non-invasive diagnostic of endometriosis; and commercial chicken rearing. I identify bacteria that exhibit …
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Isolation and partial characterization of cDNAs encoding polypeptides that interact with the capB gene products of Dictyostelium discoideum
… were tested to eliminate false positives. DNA sequencing analysis revealed that the cDNAs isolated encoded: p34/31; CABP1B, the smaller product of capA; TopA, a mitochondrial type II topoisomerase; and a previously unidentified polypeptide (clone 101) with homology to a bacterial ORF. It was …
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Characterisation of lipoproteins in staphylococcus aureus
… the infected C. elegans and subjected to RNA Sequencing, the large-scale data has provided useful information on pathogen and host activities during infection. RNA sequencing analysis showed different types of regulations and interactions of lipoprotein transcripts during host exposures to …
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The Search for Regulatory Mutations in Gitelman Syndrome
… development of whole exome and whole genome sequencing technologies has allowed geneticists to gradually uncover a wide spectrum of rare, deleterious mutations with large effect sizes, which are responsible for Mendelian diseases. Roughly 70% of human genes are single copy and have orthologs …
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An Analysis of Illegal Bushmeat Availability in Local Restaurants Located in Voi, Kenya
… Samples were collected and analyzed using mDNA sequencing analysis of the cytochrome B gene. None of the collected samples were identified as illegal game meat. The restaurants in Voi, Kenya were not a commercial outlet for illegal bushmeat trading in the local economy during the period of this …
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Metastatic Reactivation and Progression Result From Fak-Mediated Disruption of Innate Immune Signaling
… downstream of Sting activation. Single-cell RNA sequencing analysis from both patients and human xenograft models, spanning dormancy to metastasis, further substantiates this role of Fak. Genetic deletion or targeted degradation with proteolysis targeting chimera (PROTAC) of Fak, but not …
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The Phylogenetic Analysis Of Genes Encoding Specific Steps In The Glutathione Pathway Within Industrial Beer Brewing Strains: Saccharomyces Cerevisiae, Saccharomyces Pastorinus, And The Spoilage Microbe Brettanomyces
… Brettanomyces bruxellensis through single-gene sequencing analysis of the GSH pathway. It was hypothesized that the actions of these GSH genes are unique and potentially upregulated in beer brewing yeasts when compared to non-brewing yeasts strains. In order to assess this theory, GSH pathway …
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Transcriptome profiling of infected chickens with newly emerged genotype VI I Newcastle disease virus strains
… objective was to detect and perform full genome sequencing of highly virulent circulating NDVs in vaccinated chicken flocks to identify the most adequate NDV strains as vaccine candidates. Our full genome sequencing analysis on two selected isolates from vaccinated birds in Indonesia has shown …
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O-linked N-acetylglucosamine in differentiation and gene expression of mouse and human pluripotent stem cells
… Following these observations we performed mRNA sequencing analysis on human iPS cells with high O-GlcNAc levels and found gene expression to be significantly altered. Genes affected by increased O-GlcNAcylation include modulators of key neural developmental processes, for example components of …
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