Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"Sephacryl"”.
-
ISOLATION OF ELECTRON TRANSPORT COMPONENTS FROM MICROCYSTIS AERUGINOSA AND OSCILLATORIA SP
… to fractionate water-soluble proteins on Sephacryl, was successfully employed together with conventional chromatographic tools to purify the proteins of interest to homogeneity. Sephacryl is a gel matrix normally used for gel filtration, but proteins can be caused to adsorb to it under …
-
Endogenous cyclic AMP-dependent phosphorylation in vitro of cytosolic proteins from Dictyostelium discoideum
… partial purification by DE-52 cellulose and Sephacryl S-300 chromatography, cyclic AMP dependency of six cytosolic proteins was observed, with apparent subunit molecular weights of > 200,000, 110,000, 107,000, 91,000, 75,000, and 69,000. Phosphorylated bands at 107,000 and 91,000 MW were …
-
Alkylation of rat lens crystallins with iodoacetamide
… no altered chromatographic behavior on Sephacryl S-200; only alkylated βH-crystallin was altered so that it co-chromatographed with control or alkylated βL-crystallin.
-
Studies on bovine γ-glutamylamine cyclotransferase.
… precipitation, size exclusion chromatography on Sephacryl S100, anion exchange chromatography on Mono-Q under reducing conditions, isoelectric focusing of the alkylated sample, electroelution, electrophoresis, ultrafiltration, and lyophilization. The enzyme was purified more than 2,000 fold to a …
-
Immunophysiology of the uterus in cyclic ewes
… ewes. Two protein peaks were separated on a Sephacryl S-100 column. Peak one had an estimated size of ≤ 1 X 10⁵ Da, and peak two had an estimated size of 12.7 X 10³ Da. Small concentrations of peak one protein from intact follicular-phase ewes enhanced activity of T-lymphocytes in response to …
-
Action of Tyrosyl DNA Phosphodiesterase on 3'-Phosphoglycolate Terminated DNA Strand Breaks
… (from lymphoblastoid whole-cell extracts using Sephacryl S-300 gel filtration columns), we found that the recombinant enzyme had lesser 3'-PG removal activity than the partially purified native enzyme. On cloning recombinant FLAG-tagged hTdpl into human expression vectors, we observed that the …
-
Evaluation of uterine lumenal proteins in dairy cattle at known stages of the estrous cycle and in ovarian steroid treated ovariectomized cows
… collected nonsurgically via a foley catheter. Sephacryl S-200 chromatography revealed protein peaks specific to UF from cyclic cows had distribution coefficients at ambient temperature of .27 ± .02, .32 ± .02, .65 ± .03, .73 ± .06, and .91 ± .04; peaks at 4C were .35, .41 ± .04, .82 ± .05, 99 ± …
-
Heterologe Überexpression, Reinigung, biochemische Charakterisierung und Untersuchungen zur Struktur der A1 ATPase aus Methanosarcina mazei Gö1
… BioScale DEAE und einer zweiten Gelfiltration an Sephacryl S-300 HR bis zur apparenten Homogenität gereinigt. 5.Das gereinigte Enzym wies eine molekulare Masse von 355 kDa auf und setzte sich aus 5 verschiedenen Untereinheiten zusammen.Die einzelnen Untereinheiten AhaA (65 kDa),AhaB (55 kDa),AhaC …
-
PURIFICATION OF THE REGULATORY SUBUNIT OF RIBONUCLEOSIDE DIPHOSPHATE REDUCTASE FROM CALF THYMUS
… Biogel A 1.5 M (Mr = 86,900; r(,0) = 35.9 A) and Sephacryl S-300 (Mr = 99,800; r(,0) = 35.8 A). The unique role of protein B2 as the iron-containing subunit in ribonucleoside diphosphate reductase was established by treatment of the partially purified enzyme and its constituent subunits with EDTA. …
-
Tumor-induced immunosuppression: Contribution of a high molecular weight inhibitor and Prostaglandin E2
… factor was apparently >67 kd, as shown by S-200 Sephacryl chromatography and gel electrophoresis. DEAE-Cellulose chromatography suggested that the pl of the inhibitory factor was < 7.7. lsoelectric focusing revealed that the Mφ-mediated inhibitory activity differed in charge, with a pl of 6.5-7.6 …
-
Na + -translozierende rotatorische Membranproteinkomplexe aus dem anaeroben Bakterium Acetobacterium woodii: Vergleichende molekulare und biochemische Analyse des Flagellums und der F1FO-ATPase
… solubilisiert und durch Gelfiltration über Sephacryl S-400 und eine Glycerin-Dichtegradientenzentrifugation gereinigt. Das gereinigte Enzym besaß alle neun Untereinheiten. Die spezifische Aktivität der gereinigten ATPase betrug 7 U mg Protein -1 und seine molekulare Masse 600 kDa.
-
Constituintes químicos da madeira-de-lei Myracrodruon urundeuva com propriedades antioxidantes e ação contra Fungos, Bactérias e Insetos
… de exclusão molecular em coluna Hiprep 16/60 Sephacryl S-300/Äkta-FPLC. MuHL foi analisada em eletroforese em gel de poliacrilamida para proteínas nativas básicas ou ácidas e em condições desnaturantes e redutoras (SDS-PAGE). MuHL também foi avaliada quanto à natureza glicoprotéica. A AH de …