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Showing 1 to 16 of 16 for “"Separase"”.
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Die Funktion der Separase bei der Schwesterchromatiden-Trennung in Drosophila melanogaster
… sind die inhibitorischen Untereinheiten der Separasen, einer konservierten Familie von Cystein-Endoproteasen. Der Abbau der Securine führt zur Aktivierung der Separasen, die über die Spaltung einer konservierten Kohäsin-Untereinheit die Trennung der Schwesterchromatiden einleiten. Um zu …
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Separase cleaves the kinetochore protein Meikin to direct the meiosis I/II transition
… transition through the action of the protease Separase, which we demonstrate acts by cleaving the meiosis-specific kinetochore protein Meikin. Cleavage of Separase substrates such as cohesin results in their potent and complete inactivation.
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Eukaryotic chromosome segregation: New aspects of separase regulation by securin, Cdk1, PP2A and auto-cleavage
… proteolytic cleavage of chromosomal cohesin by separase. The activity of this essential but potentially also very dangerous protease must be tightly controlled. Prior to the onset of anaphase separase is kept inactive by association with either securin or cyclin-dependent kinase 1 (Cdk1) in …
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Die Funktion von Three rows bei der Schwesterchromatiden-Trennung in Drosophila melanogaster
… Schwesterchromatiden-Trennung einleitet, heißt Separase. Die Separase wird erst dann aktiviert, wenn alle Chromosomen bipolar mit dem mitotischen Spindelapparat verbunden sind. Die Aktivierung der Separase erfordert die Ubiquitinabhängige Degradation des Securins, einer inhibitorischen …
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Functional characterisation of cohesin subunit SMC3 and separase and their roles in the segregation of large and minichromosomes in Trypanosoma brucei
… segregation at anaphase. The second protein, separase, is a cysteine protease that resolves sister chromatid cohesion at the onset of anaphase and has, in other organisms, additional functions during mitosis. The T. brucei cohesin subunit, TbSMC3, localised to the nucleus as a chromatin-bound …
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The dual use of cohesin and its protector Sgo1 contributes to the choreography of the chromosome and the centrosome cycle
… 2A (Sgo1-PP2A). It is opened only in anaphase by separase-dependent cleavage of Scc1, which triggers chromosome segregation. Shortly thereafter, centrioles loosen their tight orthogonal arrangement, which licenses later centrosome duplication in S-phase. While a role of separase in centriole …
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Meiosis-specific Regulation of the Anaphase-Promoting Complex
… of securin/Pds1 is required to activate separase, a thiol protease that mediates chromosome segregation by cleavage of the cohesin complex. The first meiotic division is preceded by an extended prophase I, during which maternal and paternal chromatids undergo recombination. The …
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Regulation of Progression through Unperturbed Mitosis and in the Presence of Environmental Stress
… I have addressed the function of Drosophila Separase, a protease which has been implicated in several regulatory processes in budding yeast. Although an essential role of this protease for sister chromatid separation has been demonstrated in a wide range of eukaryotes, its involvement in …
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Studies of Aurora and Polo Kinases During Cell Division in C. elegans
… until the proteolysis of the cohesion Scc1 by separase triggers the separation of sister chromatids at anaphase. Chromosome dynamics during meiosis are more complex, as homologous chromosomes separate in anaphase I, whereas sister chromatids remain attached until anaphase II. In meiosis, …
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Analyse des meiotischen Cohesinkomplexes in Drosophila melanogaster-Weibchen
… erst beim Metaphase-Anaphase-Übergang durch eine Separase-abhängige Spaltung von Scc1/Rad21/Mcd1 abgelöst wird. Dieser exakt regulierte Mechanismus ist die Grundlage für eine korrekte Segregation und Aufteilung der Schwesterchromatiden auf die entstehenden Tochterzellen. Die Meiose stellt eine …
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Localization studies of sister-chromatid cohesion proteins MEI-S332 and RAD21 in Drosophila
… is dependent upon the activity of the separase pathway that regulates cohesin release. We have identified and characterized a key subunit of cohesin in Drosophila, DRAD21, and studied its localization in early stages of meiosis in spermatocytes.
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Regulation of mitotic exit in Saccharomyces cerevisiae
… state. The FEAR network is comprised of the separase Espl, the polo-kinase Cdc5, the kinetochore protein Slk19, the replication fork block protein Fobl, and the small nuclear proteins Spol2 and Bnsl. Genetic epistasis analyses revealed that the FEAR network consists of at least two branches. …
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Mitotic Exit Control in Budding Yeast: Regulators and Dynamics
… network including a non-proteolytic function of separase (Esp1), and the Mitotic Exit Network (MEN) indirectly activated by spindle elongation through cohesin cleavage by the proteolytic function of Esp1. The MEN contributes strongly to ME efficiency. Esp1 contributes to Cdc14 release and ME …
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Human cohesin dynamics and their regulation in M and S phase
… achieved by the action of the cystein protease separase, which cleaves cohesin at the centromeres (the primary constriction site of mitotic chromosomes) to release DNA. In vertebrates, however, the bulk of cohesin molecules, which is located on chromosome arms, is already removed from DNA in the …
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Functional gene analysis in cultured vertebrate cells using siRNA mediated gene silencing
… der Kinetochore (scc1, smc1, smc3, securin und separase) und Kinesin verwandte Motorproteine (MCAK, TPX2, HSET, KIA0622).Die Zinkfinger Metallprotease prozessiert das Zellkernprotein prelamin A durch endoproteolytische Entfernung der carboxyterminalen 16 Aminosäuren zu reifem lamin A. Die …
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The peptidyl-prolyl isomerase Pin1 is required for maintenance of the spindle assembly checkpoint
… when cohesin is endoproteolytically cleaved by separase. Activation of this giant protease requires the degradation of its two inhibitors, securin and cyclin B1, which is mediated by the anaphase promoting complex or cyclosome (APC/C), a multisubunit ubiquitin ligase, in conjunction with its …