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Showing 1 to 6 of 6 for “"Sensory Rhodopsin II"”.
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FTIR Spectroscopy of the photophobic sensory rhodopsin II/transducer complex from natronabacterium pharaonis
Archaebakterielles Sensory Rhodopsin besitzt eine transmembrane Struktur bestehend aus sieben alpha Helices. Als Chromophor dient ein Retinalmolekül, das über eine protonierte Schiff´sche Base an das Protein gebunden ist. Dieses System ist exemplarisch für Signal Transduktion und stieß in den …
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Structural and Biophysical Characterisation of Denatured States and Reversible Unfolding of Sensory Rhodopsin II
… and the kinetics for reversible unfolding of sensory rhodopsin II (pSRII), a retinal-binding photophobic receptor from Natronomonas pharaonis. pSRII is difficult to denature, and only SDS can dislodge the retinal chromophore without rapid aggregation. Even in 30% SDS (0.998 …
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Time Resolved Diffraction Studies of Structural Changes in Sensory Rhodopsin
… an appropriate cellular response. Microbial rhodopsins are a family of transmembrane proteins that harness light to perform a range of functions. These rhodopsins have been found to act as ion pumps, channels and light sensing proteins. They all utilize similar chemistry through a covalently …
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Lipidic Cubic Phase Microcrystallization and its Application in Serial Crystallography
… hits were found and optimized. These include sensory rhodopsin II from halophilic archea and reaction centre from Blastochloris viridis. For reaction centre, the method was also used in combination with crystal seeding to create a new procedure for microcrystallization. The work presented in …
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Structure-function relationship of archaeal rhodopsin proteins analyzed by continuum electrostatics
Rhodopsin proteins perform two cellular key functions: signaling of external stimuli and ion transport. Examples of both functional types are found in the family of archaeal rhodopsins, namely the proton pump bacteriorhodopsin, the chloride pump halorhodopsin and the photoreceptor sensory rhodopsin …
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Development of a saposin A based native-like phospholipid bilayer system for NMR studies
… proteins in Chapter 4. Two microbial rhodopsins, Anabaena sensory rhodopsin (ASR) and Natronomonas pharaonis sensory rhodopsin II (pSRII), were tested. The parameters for expression and purification of ASR were first screened for the optimal yield. Although incorporation of ASR …