Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Sendai virus"”.
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Understanding Intercellular Signaling During Lung Injury-Repair
… lung communicate to drive tissue repair after Sendai virus infection-induced damage, we developed a single-cell interactome method to uncover novel interactions between epithelial and non-epithelial cell types. Our interactome method employs a numeric representation of ligand-receptor …
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Charakterisierung von reptilienpathogenen Paramyxoviren und Analyse des prokaryotisch exprimierten partiellen Fusionsgens
… Identifying the isolates as paramyxoviruses showing characteristic cytopathic effect, was carried out by electronmicroscopy and RNA-PCR of two partial genes (L and F). After sequencing RNA-PCR amplicons, identical sequences were found for the reptilian paramyxoviruses (RPMV) isolated …
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Single molecule pull-down of viral RNA-protein complexes
… complexes. Specifically, the highly replicative sendai virus sub-genomic defective interfering (DI) RNA is targeted. The viral DI RNA associates with multiple viral proteins during replication, and is therefore expected to form heterogeneous RNA-protein complexes. Using the RNA sequence …
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Functional Genomic and Image-Based Screening Approaches for Probing Host-Pathogen Interactions
… of years. Interactions between bacteria or viruses and human cells, and the resulting evolved antipathogenic signaling pathways, are processes responsible for pathologies ranging from infectious diseases to autoimmune conditions and cancer. In addition, engineered designs inspired by …
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The Role of the Transcription Factor CEBPA in Regulating Lung Alveolar Type 2 Cell Fate In Vivo
… AT2 cells exhibit neonatal-like plasticity under Sendai virus infection, where Cebpa mutants, unlike their wild-type counterparts, express SOX9, proliferate more readily, and transition into KRT8/CLDN4+ cells. In parallel, our exploration into the gene regulation of Cebpa through single-cell …
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Inhibition of Cell Proliferation through Regulated Intramembrane Proteolysis of CREB3L1
… subclones that are permissive for hepatitis C virus (HCV) replication versus the non-permissive parental Huh7 cells, we identified CREB3L1 as a host factor that inhibits proliferation of virus-infected cells. Upon infection with diverse DNA and RNA viruses, including murine gamma-herpesvirus …
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Regulation of the Human Parainfluenza Virus (hPIV3) Fusion Protein
<p>Paramyxoviruses include a number of important human pathogens, including measles virus, mumps virus, and the human parainfluenza viruses (hPIV) 1-4, as well as several animal pathogens, such as Sendai virus, Nipah virus and Hendra virus. The creation of effective drugs and vaccines against this …
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Conversion of equine umbilical cord matrix mesenchymal stem cells to the trophectoderm lineage using the Yamanaka reprogramming factors
… delivery methods utilizing non-integrating viruses for transduction of target cells has provided new opportunities for regenerative medicine in humans and other species. We sought to use this technology to generate equine iPS cells to address challenges in equine regenerative medicine. …
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Untersuchungen zum Aufnahmemechanismus und intrazellulärem Transport von fusogenen und kationischen Liposomen-DNA-Komplexen für den Gentransfer
… oder entwickelt und verwendet wurden, wie Sendai-Virus Liposomen (HVJ-Liposomen), Virosomen sowie DAC-Chol und DOCSPER-Liposomen als Vertreter der kationischen Lipide. Im ersten Teil der Arbeit wurden fusogene Liposomen und Virosomen charakterisiert. Bei diesen Untersuchungen wurden …
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Host cell signaling in response to RSV infection
… gamma and IKK gamma delta in response to ssRNA viruses. I found that in contrast to IKK gamma, which is essential for both NF-kappa B and IRF3 activation, IKK gamma delta failed to activate IRF3 in response to either Sendai virus or respiratory syncytial virus (RSV). However, IKK gamma delta …
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Untersuchung der Typ I Interferon-Signaltransduktion in HPV-Onkogen immortalisierten Keratinozyten
Humanes Papillomvirus (HPV)16 gehört zu der Gruppe der �high risk� Viren, die mit der Entstehung der Zervixkarzinome assoziiert werden. Im Verlauf der HPV-vermittelten Tumorigenese wird die Kommunikation infizierter Zellen mit dem Immunsystem verändert. In der vorliegenden Arbeit wird zum ersten …
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Single molecule investigation of antiviral signaling
… respect to IFN- in cells upon infection with Sendai virus (SeV). We found that RIG-I mRNA is directly induced by viral infection in the absence of IFN at the early stages of viral infection. High throughput screening of ISGs expression following viral infection revealed other genes, which we …
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Identification and Characterization of Auxiliary Factors in Innate Antiviral Immunity
… in murine cells. Depletion of SRMS impaired virus- or dsRNA-induced expression of type I and III IFNs and ISGs in mouse embryonic fibroblasts (MEFs), mouse hepatoma hepa1-6 cells, and primary mouse lung fibroblasts. Mechanistically, SRMS acted downstream of the RLRs and their adaptor MAVS. …
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Investigating the role of a FAM111B mutation in hereditary fibrosing poikiloderma (POIKTMP) using induced pluripotent stem cell (iPSC) model
… induced pluripotent stem cells (iPSCs) via the Sendai virus vector (SeVdp) packaged with pluripotency transgenes (OCT4; SOX2; KLF4; C-MYC). The derived iPSCs successfully showed a) endogenous expression of pluripotency markers (OCT4; NANOG; TRA-1-60), b) in vitro differentiation into the three …
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Key Role of Lys63-Linked Polyubiquitination in Viral Activation of IRF3
Viral nucleic acids exposed during invasion and proliferation are detected by mammalian cells through receptors belonging to pattern-recognition receptors family (PRRs). Among PRRs, RIG-I-like receptors (RLRs), including RIG-I, MDA5 and LGP2, are responsible for sensing intracellular viral RNAs. …
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Nuclear and Plasma Membrane Properties of Macrophage Heterokaryons and Hybrids
… homokaryons were obtained through the Sendai virus-induced fusion of cells spread on a glass surface. After fusion there was a striking reorganization of cellular architecture by means of a colcemid-sensitive process. Heterokaryons were isolated through the use of differential …