Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Sema3a"”.
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Redox manipulation alters hippocampal neuron response to semaphorin 3A
… the secreted protein known as semaphorin 3a (Sema3A) and various redox reactions such as the oxidizing function of Mical proteins on f-actin that function downstream of Sema3A. However, what is less understood is how various redox conditions alter these guiding cues. This work investigates the …
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Piezo1-dependent mechanical regulation of chemical signalling in the developing Xenopus laevis brain
… led to a decrease in both semaphorin 3A (sema3A) and slit1 expression. Piezo1 depletion not only altered the chemical landscape of the developing brain, but also its mechanical properties. To understand how Piezo1 regulates tissue stiffness, I investigated microtubule acetylation, single …
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The role of semaphorin 3A signaling underlying learning and memory formation in the postnatal mouse brain
… connections. Specifically, Semaphorin 3A (Sema3A) is a secreted guidance cue protein implicated both in axonal, through the action at growth cones, and dendritic, through dendritic elongation and spine maturation, refinement. This occurs when Sema3A activates a holoreceptor complex composed …
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Neuronal filopodia borne along tips and shafts of dendrites comprise two distinct populations as evidenced by differences in structure and dynamics
… developmental cues, specifically, Semaphorin3A (Sema3A). Using confocal microscopy to visualize filopodial membrane and actin we found that shaft filopodia are shorter than tip filopodia, and show a less dense presentation along the dendrite. We then employed the quantitative phase imaging …
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Interplay between Piezo1-mediated mechanosensing and the chemical guidance cue Semaphorin3A during development of the embryonic nervous system
… altered the expression of semaphorin3A mRNA (sema3A), a chemical guidance cue known to be critical in axon pathfinding. Thus, both chemical and mechanical signals encountered by the axons were altered when Piezo1 was less abundant in the brain. To determine if tissue softening was caused by …
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Substrate stiffness tunes the neuronal response to the chemical guidance cue semaphorin3A
… neurons impacts their response to Semaphorin 3A (Sema3A), a highly conserved guidance molecule that classically functions as a chemorepellant in this system. Indeed, I found that the response of Xenopus RGC growth cones to Sema3A was significantly reduced on softer substrates. This dramatic change …
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Halting the neural progression of cancer
… were conducted in the presence of SEMA3F or SEMA3A. Our results indicated that nerves in the skin expressed NRP2 and grew towards the melanomas. There was less neuronal density in the melanomas that overexpressed SEMA3F than in control melanoma grafts. In addition, tissues from Nrp2-/- mice …
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The cue induced axonal nascent proteome and its translational control mechanisms in neural wiring
… stimulated by different cues (Netrin-1, BDNF, Sema3A) each show a signature set of up/down newly synthesised protein (NSP) changes (>100) within 5 min. Remarkably, conversion of Netrin-1-induced responses from repulsion to attraction triggers opposite translational regulation for 73% of a …
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Aberrant assembly and function of a hippocampal circuit in a genetic mouse model of schizophrenia
… its downstream target pCREB. Finally, Nrp1 and Sema3A, guidance cues that are regulated by cAMP, are altered in the Disc1Tm1Kara mutant mice. Overall, this mouse is a valuable model of a known genetic variant of schizophrenia susceptibility and adds to our understanding of the pathology of the …
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NOVEL INSIGHTS INTO GENETIC AND ENVIRONMENTAL DETERMINANTS SHAPING GNRH NEURON BIOLOGY AND RELATED REPRODUCTIVE DISORDERS
… binding of axon guidance molecules like VEGF and SEMA3A. While mutations in HS6ST1 have previously been reported in IGD patients, the role of HS6ST2 remained uncharacterized. Using a murine model, it was demonstrated that combined, but not single, loss of Hs6st1 and Hs6st2 leads to increased …