Global ETD Search
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Showing 1 to 6 of 6 for “"Self-cleaving"”.
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Ribozymes of the RNA World: Investigating Ancient Enzymes through Modern Self-Cleaving Ribozymes.
… of ribozymes in the modern world, called the self-cleaving ribozymes. They catalyze the cleavage of a specific phosphodiester bond in RNA. We investigated the different catalytic strategies used by one such ribozyme, called the hammerhead ribozyme, and uncovered how RNA can utilize …
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Search for new types of deoxyribozymes and development of human topoisomerases inhibitors on the basis of oligonucleotides
… of new types of deoxyribozymes that possess DNA self-cleaving activity. Certain guanine-rich oligonucleotides with potential to form G-quadruplexes were constructed and their self-cleaving activities are examined during our investigations. Our studies demonstrated that certain guanine-rich …
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Investigating and expanding the functionality of RNA catalysts: Studies of the hepatitis delta virus, the hammerhead, and the aminoacyl-tRNA synthetase-like ribozymes
… used for probing the active site of two small self-cleaving ribozymes, the hepatitis delta virus (HDV) ribozyme and the hammerhead ribozyme. Results presented here suggest that divalent metal ions play critical roles in the catalytic mechanisms of both the HDV and the hammerhead ribozymes. In …
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Development of Bacillus subtilis spores and cells for surface display of proteins
… from the SSp DnaB, which was then used as self-cleaving tag for purification by shifting the pH and/or temperature conditions, with no addition of any proteases or thiol reagent (Mathys et al., 1999). To construct the system, the mini-intein was fused downstream of the CotB protein, …
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DEVELOPMENT OF AVIAN PARAMYXOVIRUS VECTORED VACCINES
… with various proteins of NDV vector, with a 2A self-cleaving peptide, a furin cleavage site, or both, placed between the AIV and NDV sequences for separation of the two proteins. Among different constructs tested, we only recovered viable viruses with AIV HA fused C-terminally to the NDV HN …
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Biochemical and Immunological Mechanisms Underlying Differential Interaction of Superantigens with Host Immunogenetic Factors in Streptococcal Sepsis
… BLS cells, we utilized the novel technology of ‘self cleaving’ 2A peptide to generate multicistronic retroviral vectors. The constructs were cloned into pHSPG retroviral vector and packaged into HSPG viral particles that were used to transduce (BLS). DR or DQ and chains were expressed as a single …