Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Securin"”.
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Eukaryotic chromosome segregation: New aspects of separase regulation by securin, Cdk1, PP2A and auto-cleavage
… is kept inactive by association with either securin or cyclin-dependent kinase 1 (Cdk1) in conjunction with cyclin B1. Only when all chromosomes interact properly with the mitotic spindle apparatus does the anaphase promoting complex or cyclosome (APC/C), a multisubunit E3 ligase, mediate the …
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The peptidyl-prolyl isomerase Pin1 is required for maintenance of the spindle assembly checkpoint
… requires the degradation of its two inhibitors, securin and cyclin B1, which is mediated by the anaphase promoting complex or cyclosome (APC/C), a multisubunit ubiquitin ligase, in conjunction with its essential co-activator Cdc20. The spindle assembly checkpoint (SAC) is a surveillance mechanism …
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Die Funktion der Separase bei der Schwesterchromatiden-Trennung in Drosophila melanogaster
… funktionelle Gemeinsamkeiten zur Klasse der Securine auf. Es wird wie andere Securine am Metaphasen/Anaphasen-Übergang proteolytisch abgebaut, wodurch die Trennung der Schwesterchromatiden ermöglicht wird. Die Securine sind die inhibitorischen Untereinheiten der Separasen, einer konservierten …
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Die Funktion von Three rows bei der Schwesterchromatiden-Trennung in Drosophila melanogaster
… erfordert die Ubiquitinabhängige Degradation des Securins, einer inhibitorischen Untereinheit der Separase. Weitere Mechanismen der Separase-Regulation sind noch nicht vollständig verstanden. Das Securin von Drosophila melanogaster ist das Protein Pimples (PIM). Die Separase (SSE) von D. …
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CDK control of mitotic progression in Saccharomyces cerevisiae
… to be required for the timely degradation of Securin/Pds1 at the metaphase-anaphase transition, demonstrating a requirement for mitotic CDKs in APC/C-Cdc20 activation in vivo. Second, mitotic CDK activity was necessary for the elongation of the mitotic spindle in anaphase. This requirement for …
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Meiosis-specific Regulation of the Anaphase-Promoting Complex
… (APC/C). APC/C-dependent destruction of securin/Pds1 is required to activate separase, a thiol protease that mediates chromosome segregation by cleavage of the cohesin complex. The first meiotic division is preceded by an extended prophase I, during which maternal and paternal chromatids …
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Harnessing the Anaphase-Promoting Complex for Targeted Protein Degradation
… the destruction of numerous proteins including Securin, Cyclin B1 and Aurora kinase A. Most natural APC/C substrates contain multiple copies of short linear motifs (SLiMs), termed degrons, that serve as APC/C-recognition sequences. My research approach involved systematically dissecting various …
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Molecular Mechanisms of Mitotic Checkpoint Complex Assembly onto Kinetochores
… of two key cell cycle regulators, cyclin B and securin. The MCC is composed of BubR1, Cdc20 and Mad2, and while their assembly is an intrinsically very slow process, in cells it is catalytically activated. Recent work points towards hierarchical recruitments of SAC proteins onto the outer …
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The developmental role and regulation of the Anaphase-Promoting Complex/Cyclosome in Drosophila meiosis
… of PIMPLES, the Drosophila homolog of Securin. Both post-transcriptional and post-translational regulation of cort result in expression of CORT protein being restricted to the meiotic divisions. Cytoplasmic polyadenylation of cort mRNA is tightly correlated with appearance of the …
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Mechanism of APC/C activation and substrate specificity in mitosis
… defined order. At mitosis onset, degradation of securin and cyclin B1 is inhibited by the spindle assembly checkpoint, exerted by the mitotic checkpoint complex (MCC), whereas both cyclin A2 and Nek2A are not subject to MCC inhibition. The aim of the second part of the thesis was to elucidate the …
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Functional gene analysis in cultured vertebrate cells using siRNA mediated gene silencing
… Proteine der Kinetochore (scc1, smc1, smc3, securin und separase) und Kinesin verwandte Motorproteine (MCAK, TPX2, HSET, KIA0622).Die Zinkfinger Metallprotease prozessiert das Zellkernprotein prelamin A durch endoproteolytische Entfernung der carboxyterminalen 16 Aminosäuren zu reifem lamin …
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Identification and functional characterisation of novel APC/C interacting proteins
… cycle regulators such as cyclin A, cyclin B, securin and S-phase kinase-associated protein 2 (SKP2) for proteasomal-dependent degradation. APC/C substrates and coactivators are aberrantly expressed in many cancers. It is thought that the APC/C can also regulate cellular proliferation by …
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Regulation of Anaphase Promoting Complex/Cyclosome to Control M Phase Exit
… of anaphase, the APC/C targets cyclin B and securin for degradation, initiating chromosome separation and mitotic exit. Regulation of APC/C activity is critical for a functional cell cycle, and this is largely mediated by cytostatic factor (CSF) activity and the Spindle Assembly Checkpoint …
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TRAF Regulation of Caspase-2-Dependent Apoptosis in Response to DNA Damage
… critical pro-mitotic proteins, like cyclin B and securin, to promote mitotic exit. Although the function of the MCC is well understood, its regulation is not, especially in regard to protein phosphatases To investigate this, we activated the spindle checkpoint with microtubule inhibitors and then …