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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Secretion signal"”.
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The structural characterization of the Saccharomyces cerevisiae alpha mating factor secretion signal for recombinant protein secretion in Pichia pastoris
… The most successful and commonly used secretion signal leader in Pichia pastoris has been the MAT α prepro secretion signal. However, limitations exist as some proteins cannot be secreted efficiently even with the MAT α prepro secretion signal. Some strategies to enhance secretion …
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Structural characterization of the MATα prepro-peptide secretion leader in Pichia pastoris
… modifications. The most commonly utilized secretion signal leader in P. pastoris is the MATα prepro signal leader, originally found in S. cerevisiae. However, because some proteins cannot be secreted efficiently by P. pastoris, strategies to enhance secretion efficiency have involved the …
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The effects of cis- and trans-acting mutations on recombinant protein secretion in Pichia pastoris
… tools such as the cis -acting factors MATα secretion signal and MBP fusion partners, and trans-acting modifications such as the bgs mutants have increased heterologous protein secretion. Although these techniques have already been used, their effects on the protein secretory pathway have yet …
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Effect of Emb-9 and Let-2 Mutations on the Proper Assembly of Basement Membranes and on the Morphology of Body Wall Muscle Cells in Caenorhabditis Elegans
… presence of a myo-3 promoter, and β1 integrin secretion signal/transmembrane domain. Analysis of transgenic strains demonstrated that mutations in either the α1 or α2 chains of collagen type IV cause a change in shape and organization of muscle cells. Immunofluorescence analysis of all …
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The recombinant expression and localization of TvCP2 of trichomonas vaginalis
… Previous data gives strong evidence of the secretion of cysteine protease CP4 and hints at the possibility of CP2 secretion as well; however, our results show no co-localization between CP2 and CP4 in T l-CP2 over expressing transfectants, suggesting separate trafficking and different roles. …
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Molecular intervention in Pierce's Disease
… The expression cassette that include the pelB secretion signal was created and tested for its efficiency in secreting the 4XfMopB3 antibody in E. coli and P. agglomerans. The 4XfMopB3 antibody was successfully expressed and secreted in E. coli while it was only expressed in P. agglomerans.
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Low level Tc1 transposition and Tc1-induced mutations in dpy-5
… or protein patterns; however, a potential secretion signal peptide has been identified. Northern analysis indicates that the gene is developmentally expressed, correlating well with the onset of the Dpy-5 phenotype. Analysis of DNA sequences upstream from the dpy-5 gene reveals the presence …
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Lactobacillus strategies to reduce Campylobacter jejuni colonization of broiler chickens
… FlpA Domain II under a promoter fused to a secretion signal with or without an anchoring signal from the mucus-binding protein of Lactobacillus acidophilus NCFM. Additional work must be conducted to evaluate the exterior protein expression and in vivo antigen-immune response within broiler …
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Construction of a recombinant immunotoxin
… in the periplasm of E.coli (TOPP2) using the secretion signal pelB . Expression of the foreign protein was monitored by western blot using a monoclonal antibody which recognises the FLAG peptide encoded at the carboxy terminal region of the gene construct. Expression of the recombinant …
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Characterization of the MATα pre-/pro- peptide by mutagenesis as a means to optimize secretion in pichia pistoris
… cerevisiae</em>'s <em>MAT</em>α <em>pre-pro</em> signal leader (<em>MAT</em>α) has led to increased protein secretion in most cases. <em>MAT</em>α is thus used in the production of 90% of all proteins secreted in <em>P. pastoris</em>. However secretion efficiency still remains a problem.</p> <p>It …
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Structure and dynamics of Pseudomonas aeruginosa ICP
… is supported by the presence of a putative secretion signal peptide at the N-terminus of PA-ICP which may be involved in exporting the protein to target exogenous CPs. In order to shed light on the biological function and inhibitory specificity of PA-ICP, the structure and backbone dynamics …
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A genetic and molecular analysis of the bli-4 locus of Caenorhabditis elegans, an essential gene encoding KEX2-like subtilisin-typw serine endoproteases
… blisterin B gene product has a potential secretion signal peptide atits amino terminal. Blisterin C has a potential Tran membrane domain near its carboxyl terminal while blisterin A and B lack this domain. The blisterins share significant sequence identity with kex2-like serine …
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Evolution of a gene for pathogenicity: endo-pectate lyase
… unmodified protein of 281 amine acids. A typical secretion signal peptide is not present.
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Discovery of an extracellular stress sensory protein in Beauveria bassiana and identification of photolyase encoding phr-1 sequences in five entomopathogenic fungi
… size (26 kDa), potential glycosylation sites and secretion signal peptide. In addition, other features such as the high proportion of cysteine residues and internal amino acid repeats will be discussed. The elf gene was inserted into an expression vector and introduced into the methylotrophic …
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Identification of host proteins targeted by the flax rust effector protein AvrL567
… iA2 is a fungal protein with an N-terminal secretion signal peptide and was termed Secreted Interactor of AvrL567 Two (SIA2). There are homologues of SIA2 in Melampsora larici-populina and Puccinia graminis f.sp tritici but their functions are unknown. SIA2 failed to show interaction with …
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Identification of host proteins targeted by the flax rust effector protein AvrL567
… iA2 is a fungal protein with an N-terminal secretion signal peptide and was termed Secreted Interactor of AvrL567 Two (SIA2). There are homologues of SIA2 in Melampsora larici-populina and Puccinia graminis f.sp tritici but their functions are unknown. SIA2 failed to show interaction with …
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Construction and analysis of novel controllable expression vectors for Bacillus subtilis
… transcription, translation, protein folding and secretion mechanisms, genetic manipulation, and large-scale fermentation has been acquired. But so far, efficient and inexpensive expression vectors for B. subtilis are still missing. To fill this gap, a glycine-inducible expression system and a …
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Bioengineering of S-layers: molecular characterization of the novel S-layer gene sslA of Sporosarcina ureae ATCC 13881 and nanotechnology application of SslA protein derivatives
… to the native SslA protein, suggesting that SslA secretion signal is not functional in E. coli. Heterologously produced SslA derivatives with truncations of N-, C- or both N- and C-terminal parts were shown to self- assemble in vitro, although the size of self-assembly structures was different …
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Regulation of Wingless secretion, distribution and signaling
… embryonic patterning and adult homeostasis. Wnt signaling is highly regulated in all aspects, including the biogenesis and processing of active Wnt ligands, Wnt secretion and gradient formation on the cell surface and in the extracellular matrix, and perception and signal transduction within the …