Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Second-Harmonic Generation Imaging"”.
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Mueller-matrix-based analysis for second-harmonic generation imaging
Second-harmonic generation microscopy takes advantage of second-order nonlinear property for imaging of biological structures. Invoking quantitative image analysis techniques facilitates the assessment of tissues in varying states. This thesis presents a quantitative description of the relative …
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Application of quantitative second-harmonic generation imaging to dynamic systems
… is to report the development of a quantitative second harmonic generation technique that quantifies the 2 dimensional spatial orientation of collagen fiber samples under dynamic conditions. The technique is demonstrated both for a well aligned tendon sample and a randomly aligned, sparsely …
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Identification of quantitative second-harmonic generation imaging metrics for collagenous tissue
Second-harmonic generation (SHG) imaging has high specificity to collagen, sub-micrometer resolution, and optical sectioning capability. It is applied to various unstained collagenous specimens to explore the microstructure. Since collagen is one of the most abundant proteins in biological tissue, …
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Quantifying collagen-based biological tissues using Fourier-transform-second-harmonic generation imaging
… report the generalization of Fourier transform-second-harmonic generation (FT-SHG) imaging to quantify the arrangement of collagen fibers in biological tissues in 3D. Collagen is the primary structural protein in the human body. Depending on the spatial arrangement of collagen fibers, …
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Quantification of collagen fiber organization in biological tissues at cellular and molecular scales using second-harmonic generation imaging
… symmetry, thus allowing for the process of second harmonic generation (SHG) when exposed to intense incident light. Consequently, SHG microscopy has been frequently employed in medicine and biology to obtain high-contrast 3D images of collagen fibers without the need for staining. …
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Improving the delivery and efficacy of molecular medicine via extracellular matrix modulation : insights from intravital microscopy
… treatment, we adapted and further developed the imaging technique of intravital second harmonic generation microscopy. Using second harmonic generation imaging in combination with a fluorescently labeled gene therapeutic vector, we demonstrated that the spread of these viral vectors within tumors …
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Optical characterisation of the interaction between calcium-silicate based dental restorative materials and dentine
… aged in an in-vitro model. Fluorescence lifetime imaging and second harmonic generation imaging were also used for the characterisation of re-mineralisaion and collagen denaturation respectively. For the chemical analysis, Raman spectroscopy was applied to analyse the chemical composition of the …
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Second-harmonic generation-based Mueller matrix polarization analysis of collagen-rich tissues
… of the cell and its surrounding matrix. Second-harmonic generation (SHG) microscopy is especially well-suited as an image acquisition technique, due to its specificity to the non-centrosymmetric structure of collagen, and inherent confocality which enables three-dimensional sectioning. …
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Development of non-invasive techniques for bladder cancer diagnosis and therapy
… of collagen analysis in bladder cancer using second harmonic generation imaging, the application of bladder tissue computer simulation to better elucidate fluorophore properties, and progress in novel laser therapy techniques for bladder cancer. The ultimate goal of this research is the …
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Tearing of Vaginal Tissue under Biaxial Loading: Implications for Women's Health
… a multiphoton microscopy technique known as second-harmonic generation imaging to investigate the change in organization with mechanical loading. Furthermore, imaging is performed in the near-regions of tears to reveal the relationship between collagen fibers and tearing resistance. The data …
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In vitro characterization and in vivo assessment of equine tendon-derived progenitor cells
… of the repair tissues with Fourier transform-Second Harmonic generation imaging demonstrated that collagen alignment was significantly better in TDPC group than in the saline controls. In summary, TDPC administration improved the histological and biomechanical properties of collagenase-induced …
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Damaged Collagen Detection and A Novel Approach to 1,3-Dipolar Cycloaddition Reactivity: Research at the Interface of Chemistry and Biology
… This type of disease can cause fibrinoid degeneration or changes in the collagen fibers that denature the collagen triple helix. Collagen mimetic peptides (CMPs), variants that are monomeric in solution but have high ability to anneal with damaged collagen, are a growing tool in the …