Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Second harmonic generation microscopy"”.
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Application of beam shaping in second-harmonic generation microscopy
Second-harmonic generation (SHG) microscopy, based on the second-order nonlinear susceptibility, has been established as a sub-micrometer resolution, label-free optical imaging method. Due to its selectivity for non-centrosymmetric structures and inherent confocality, SHG imaging has been used to …
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Wide-field Polarimetric Second Harmonic Generation Microscopy for Histology Imaging
Nonlinear optical microscopy provides several contrast mechanisms including second harmonic generation (SHG), third harmonic generation (THG) and multiphoton excitation fluorescence (MPEF) that can contribute with additional information to hematoxylin and eosin (H&E) stained tissue histopathology, …
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UNDERSTANDING AND EVALUATING CRYSTAL POLYMORPHISM BY SECOND HARMONIC GENERATION MICROSCOPY
… factor to trap the rare metastable polymorphs. Second harmonic generation microscopy (SHG) possesses enough sensitivity to detect sub-micrometer size chiral crystals selectively and has the potential for use in crystal nucleation studies.
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Coupling qualitative and quantitative analyses of pharmaceutical materials enabled by second harmonic generation microscopy
… stability, and efficacy of a drug product. Second harmonic generation (SHG) microscopy has recently been developed as a selective and rapid method for imaging crystallinity in drug formulations. While SHG microscopy can enable the high signal-to-noise (SNR) detection of crystallinity, the …
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Elastic Characterization of <i>ex vivo</i> Human Prostate Tissue Using Vibration Optical Coherence Elastography and Second Harmonic Generation Microscopy
… a total of 840 core biopsies. Additionally, the second harmonic generation (SHG) microscopy focused on the cells and surrounding matrix within the prostate tissue. The SHG study with 42 core biopsies was intended to investigate the reason of stiffness increase of the cancerous tissue in the …
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Mueller-matrix-based analysis for second-harmonic generation imaging
Second-harmonic generation microscopy takes advantage of second-order nonlinear property for imaging of biological structures. Invoking quantitative image analysis techniques facilitates the assessment of tissues in varying states. This thesis presents a quantitative description of the relative …
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Improving the delivery and efficacy of molecular medicine via extracellular matrix modulation : insights from intravital microscopy
… after photobleaching combined with intravital microscopy to quantitate the transport properties of the tumor interstitium. We found that the presence of fibrillar collagen correlated with hindered diffusion in vivo, and also in vitro, in collagen gels prepared to mimic tumor extracellular …
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Identification of quantitative second-harmonic generation imaging metrics for collagenous tissue
Second-harmonic generation (SHG) imaging has high specificity to collagen, sub-micrometer resolution, and optical sectioning capability. It is applied to various unstained collagenous specimens to explore the microstructure. Since collagen is one of the most abundant proteins in biological tissue, …
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Sparse Representations and Nonlinear Image Processing for Inverse Imaging Solutions
… and image inpainting applications. The second problem addressed in this work involves image enhancement for detection and segmentation of objects in images. We exploit the concept that even though data from various imaging modalities have high dimensionality, the data is sufficiently …
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Ultrafast Probing of Nonlinear Dynamics in Quantum Materials: Beyond Linear Response Probes
… spectroscopy, transient absorption spectroscopy, second harmonic generation microscopy, and high field THz excitation spectroscopy, are capable of probing the properties of quantum materials that are not accessible with linear response techniques. By employing these techniques, this thesis …
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What to Expect when Mice Are Expecting: Pregnancy-Driven Changes in the Reproductive Tract
… permanent changes in shape near the cervix. The second study pressurized the reproductive tracts of mice outside of the body to describe how the different organs stretched as a unit. The reproductive tracts were more flexible at late pregnancy and postpartum than the virgin ones, and less …
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Second-harmonic generation-based Mueller matrix polarization analysis of collagen-rich tissues
… of the cell and its surrounding matrix. Second-harmonic generation (SHG) microscopy is especially well-suited as an image acquisition technique, due to its specificity to the non-centrosymmetric structure of collagen, and inherent confocality which enables three-dimensional sectioning. …
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Development and applications of high speed and hyperspectral nonlinear microscopy
Nonlinear microscopy refers to a range of laser scanning microscopy techniques that are based on nonlinear optical processes such as two-photon excited fluorescence and second harmonic generation. Nonlinear microscopy techniques are powerful because they enable the visualization of highly …
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Understanding Hearing Loss in Osteogenesis Imperfecta
… III, and applied synchrotron microtomography, second harmonic generation microscopy, Raman spectroscopy, nanoindentation, X-ray fluorescence microscopy, and auditory brainstem response tests.</p> <p>Our findings show that while <em>oim/oim</em> ossicles show no alterations in tissue mechanics, …
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Label-free breast histopathology using quantitative phase imaging
… phase imaging (QPI). QPI is a label-free microscopy technique where image contrast is generated by measuring the optical path-length difference (OPD) across the specimen. OPD refers to the product of the refractive index and thickness at a point in the field of view. Since this measurement …
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Enhanced Proteomic Methods to Decipher How the Novel Protein SNED1 Modulates the Extracellular Matrix
… neural crest cell migration. On the other hand, second harmonic generation microscopy revealed that presence of SNED1 altered the ECM organization, but the SNED1-dependent changes in the matrisome was not yet identified. Thus, I hypothesized that SNED1 modulates the ECM architecture by …
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Second Harmonic Generation – A Novel Approach in Retinal Imaging
<p>Here we present the utilization of Second Harmonic Generation (SHG) for label-free imaging of microtubules (MTs) in the retinal nerve fiber layer (RNFL). MTs are an important part of axonal cytoskeleton, providing structural support and serving as a railroad in intracellular transport. We …