Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Sec61"”.
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Fucntional Analysis of Sec61beta, a Component of the Sec61 protein Translocation Channel at the Endoplasmic Reticulum
Der Sec61-Komplex vermittelt über eine wäßrige Pore die Translokation von sekretorischen Proteinen ins ER-Lumen sowie die Insertion von Membranproteinen in die ER-Membran. Der Komplex besteht aus einer a-Untereinheit, die den eigentlichen Translokationskanal bildet, sowie einer b- und einer …
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Biogenesis of Multipass Membrane Proteins
… inserts into the membrane sequentially via the Sec61 translocation channel. This framework was primarily extrapolated from mechanistic studies of a few single-pass membrane proteins, but direct analyses of multipass membrane proteins were limited. This thesis reports efforts to understand the …
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Structural Studies of Protein Transport
… study of how the eukaryotic SecY channel, Sec61, interacts with the transmembrane domains (TMDs) of nascent IMPs. Sec61 was co-purified with stalled ribosome-nascent chains which encoded TMDs of each orientation, N-in and N-out, that appear biochemically to bind to a recognition site in …
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Mechanisms of multipass membrane protein biogenesis
… TMD1, while insertion of the next TMD required Sec61. Finally, EMC necessity could be by- passed by enforcement of TMD1 topology via an N-terminal signal peptide. Following accurate insertion of TMD1, we define the engagement of a newly identified intramembrane chaperone protein complex that we …
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Identification and characterization of rickettsial proteins at the host-pathogen interface
… reticulum where it interacts with the host Sec61 translocon. Taken together, this work highlights the elusive nature of rickettsial effectors while offering new ways to probe the unique biology of these bacterial pathogens.
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Changes in the Rpb3 Interactome Caused by the Deletion of RPB9 in Saccharomyces cerevisiae
… interactions with Rtr1, Sen1, Vtc4, Pyc1, Tgl4, Sec61, Tfb2, Hfd1, Erv25, Rib4, Sla1, Ubp15, Bbc1, and Hxk1. The most prominent of these hits are Rtr1, an Rpb1 C-terminal domain phosphatase linked to transcription termination, and Sen1, an RNA/DNA nuclease that terminates transcription. In …
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Identifizierung von Faktoren, die funktionell mit dem Sac1p-Protein in Saccharomyces cerevisiae interagieren
… Sekretionsmutanten des Translokationsapparates (SEC61 und SEC63) ist. Diese Ergebnisse unterstreichen die regulatorische Funktion von Sac1p im ATP-Transport in das ER Lumen. Um weitere Aufschlüsse über die Funktionen von Sac1p zu erhalten, wurde ein synthetisch letaler Screen mit sac1D …
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Protein degradation from the endoplasmic reticulum in yeast
… We used existing strains that carry mutations in SEC61 that have demonstrated impaired degradation of the soluble ER protein, CPY*. We find that these strains demonstrate no defect in the degradation of the transmembrane protein HLA-A2, indicating that transmembrane and soluble proteins may …
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Design, Synthesis, and Evaluation of Covalent CADA Analogues as Potent TLR4 Downmodulator for the Treatment of Opioid Use Disorder
… downregulate other proteins going through the Sec61 pathway, including TLR4. This work aims to design and synthesize new covalent CADA analogues targeting the SP of TLR4 to address OUD. This study explores the synthesis of different covalent CADA analogues targeting the SP of TLR4, which …
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Investigating the Unique Mechanism of Action of CADA Analogs for Down-Modulating CD4, Sortilin, and ACE2
The small molecule cyclotriazadisulfonamide (CADA) has been found to inhibit the replication of human immunodeficiency virus (HIV) and human herpesvirus type 7 in cell cultures. It works by down-modulating human CD4 (hCD4) and blocking co-translational translocation of the nascent protein across …