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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 42 for “"Seahorse"”.
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Understanding Seahorse Distribution and Ecology in Malta through Citizen Science
Two seahorse species (Hippocampus guttulatus and Hippocampus hippocampus) are frequently encountered on the Maltese archipelago. Both species are considered to be endangered and are globally data deficient. They are under-studied in Maltese waters when compared to data published from Mediterranean …
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The effects of herbal extracts and compounds on the glucose metabolism in Hep G2 cells
… of glycogen content, glucose release, and Seahorse metabolic analysis. In studies on glucose uptake using flowcytometry, metformin increases 1.8-fold, goldenseal stimulates 2-fold, and berberine augments 3.4-fold more glucose uptake than the control (p<0.001). In glucose transporter-1 …
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Application of the latching control on a wave energy converter
… to address the performance of the COPPE/Seahorse device in the nearshore Rio de Janeiro. The code is adapted to model a specific PTO system consisting of a gearbox and a rotational generator. An adapted constant-delay latching control aiming at the optimization of the WEC power generation …
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Mitochondrial Metabolism Elucidated by Rapid Fractionation from Tissue
… shown enrichment of mitochondrial metabolites. Seahorse and Oroboros analysis have shown that the mitochondria are functional and capable of coupled respiration. Data is presented on how the method optimisation was analysed and developed. This largely reduced time frame gives the advantage over …
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Host-Defense Function of Anuclear Polymorphonuclear Neutrophil in Lung Injury
… in velocity. Upon further investigation using seahorse assay, we observed a loss in mitochondrial functions. Mitochondrial transplantation restored the host defense and anti-inflammatory function of PMNcyto. An increase in ATP production, enhanced bactericidal efficiency, and prevented lung …
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LINEAGE TRACING REVEALS PAEP AS A CHEMOTHERAPY-INDUCED METASTATIC DRIVER AND EXPOSES METABOLIC VULNERABILITIES OF CIRCULATING TUMOR CELLS IN BREAST CANCER
… analyses. Targeted metabolomics and Seahorse assays profiled metabolic states. Functional tests included PAEP knockdown and transient pharmacologic modulation of mitochondrial activity in freshly isolated CTCs. AC reduced clonal diversity at the primary site but selectively enriched …
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The Role of Angiotensin II in Skeletal Muscle Metabolism
… was measured using an XF 24 Flux Analyzer (Seahorse Bioscience) and reactive oxygen species measured using confocal microscopy. The clinical study involving the drug Benicar ® investigated the metabolic effects of blocking angiotensin II on skeletal muscle fatty acid oxidation, glucose …
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Physiological Oxygen and Media Conditions Influence Breast Cancer Hallmarks in Vitro
… and optimized a novel protocol for performing Seahorse metabolic flux analysis under hypoxic conditions, enabling more accurate assessment of metabolism in low-O₂ environments. Collectively, our results demonstrate that both oxygen levels and media composition profoundly influence cancer cell …
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Investigation of Microbiome Metabolites and Mitochondrial Function in a model of Non-Alcoholic Fatty Liver Disease
… a number of bioenergetic parameters with the Seahorse extracellular flux analyser in control cells and cells exposed to PAA (100 µM and 200 µM) or TMAO (20 µM, 50 µM, 100 µM and 200 µM). PAA and TMAO led to an increase in lipid deposition in HepaRG cells. TMAO caused a 42% increase in lipid …
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Mitochondrial function in atherosclerosis and vascular smooth muscle cells
… ApoE-/- mouse aortas, assessed through a 24-well Seahorse extracellular flux analyser, was not affected prior to the development of atherosclerotic plaques. Developed human carotid atherosclerotic plaques were dissected into defined regions including healthy media, shoulder region, fibrous cap and …
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Hypoxia and the regulation of host responses to acute bacterial pulmonary infections
… by RNA-sequencing and functional glycolysis by Seahorse was performed on blood leucocytes. Results – Concurrent exposure to hypoxia and infection resulted in neutrophil-mediated morbidity and mortality. Acute hypoxia caused rapid utilisation of glucose, glycogen and fat stores resulting in …
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Isolation and characterisation of anti-diabetic pharmacological activities of phytoestrogens and components of Moringa peregrina (Forssk) Fiori
… basic metabolic activity of HepG2 cells using a Seahorse XF-24 analyser. The three components chosen were O-ethyl 4-[(α-L-rhamnosyloxy) benzyl] thiocarbamate (E), ȕ-sitosterol from the phytosterols family and Rutin. Real-time monitoring of cell metabolism by a Seahorse XF-24 auto analyser after …
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Caracterização da resposta imunometabólica frente a infecção por Leishmania amazonensis e Leishmania braziliensis em macrófagos de camundongos BALB/C
… of infected macrophages in real time using Seahorse assays. The production of cellular reactive oxygen species (ROS) was measured using the “CellROX” probe, and mitochondrial reactive oxygen species (mtROS) were measured using the “MitoSOX” probe. RESULTS: Murine macrophages treated with …
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Interplay of Brain Derived Neurotrophic Factor and Protein Kinase A in Modulating Mitochondrial Structure and Function in Brain Homeostasis and Disease
… neurons in a rigorous manner by multiplexing the Seahorse XFe24 analyzer with ImageXpress® Nano high content imaging microscopes. By using this novel methodology, I was able to characterize the temporal effects of BDNF on brain energy production and connectivity with high resolution. Overall, my …
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Characterisation of endothelial cell metabolic plasticity and vascular remodelling in the retina
… of in vitro metabolism were assessed using the Seahorse XF system and qPCR to highlight potential changes in metabolic pathways. Functional assays including 3D-Matrigelexperiments, Clonogenics, proliferation, migration, and barrier integrity were also implemented to ascertain what changes to …
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Investigating the effects of Wnt/β-catenin Signalling on Melanoma Cell Metabolism and Mitochondrial Dynamics
… cells. Cellular metabolic analysis using the Seahorse Bioscience XFe96 Analyzer suggests that Wnt/β-catenin mediated mitochondrial fusion may cause a global down-regulation of cellular energy metabolism in melanoma cells. This is supported by biochemical analysis of citrate synthase and …
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Abrogation of serine metabolism exacerbates intestinal epithelial inflammation via C/EBPβ-NF-κB pathway
… mitochondrial function as assessed by the Seahorse assay. Combining RNAseq and proteomic analysis, we identified C/EBPβ as the critical regulator in mediating cellular inflammation under total serine starvation via the NF-κB pathway. In this study, we revealed that disruption of serine …
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Effects of Trimethylamine N-Oxide on Mouse Embryonic Stem Cell Properties
… high-dose TMAO treatment was measured with a Seahorse XFe96 Analyzer. TMAO treatment significantly decreased the rate of glycolysis, and it increased the rate of compensatory glycolysis upon inhibition of oxidative phosphorylation (OxPHOS). It also significantly increased the rate of OxPHOS, …
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Cellular Reprogramming in Skeletal Muscle after Repeated Exposures to Endotoxin
… oxidative capacity via an XF24 Flux Analyzer (Seahorse Bioscience) as measures of the metabolic response. Tolerance to LPS was observed in response to low- and high-doses with MCP-1 mRNA transcription but not IL-6. Changes in PGC1-° and mitochondrial OCR exhibited a tolerant effect in response …
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