Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Salt bridge"”.
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Probing the conformational changes of the yeast mitochondrial ADP/ATP carrier
… to the substrate binding site is regulated by salt bridge networks on either side of the central cavity, called the matrix and cytoplasmic salt bridge network. It has been proposed that during transport the salt bridge networks disrupt and form in an alternating way, opening and closing the …
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Force-dependent changes in alpha-catenin conformation
… studies found that a cooperative network of salt bridges stabilizes the autoinhibited conformation of the M domain. Studies described in this dissertation established that disruption of salt bridges within the M domain triggers the activation (unfurling) of α-catenin to bind vinculin, both at …
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Electrochemistry and Electrokinetics in Microchannels
… of a miniaturized reference electrode and salt bridge. The purpose of a salt bridge is to allow ionic conduction between the reference and working electrodes while maintaining a physical separation between the two systems. Macro reference electrode and salt bridge techniques are difficult …
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Examination of intramolecular interactions between residues of the cys-loop GABA receptor Hco-UNC-49 from Haemonchus contortus
… a template. These residues are predicted to form salt bridges in Hco-UNC-49B which may contribute to structure, function, and molecular interactions with ligands. Each amino acid was mutated to an alanine, followed by a mutation to a similar charged amino acid, and finally a swap between the two …
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The relationship between structure and thermostability of a nitrile hydratase from Goebacillus pallidus RAPc8
… of electrostatic interactions, particularly salt bridges and hydrogen bonds in enhancing the thermostability of the mutant NHases. The difference in the free energy of activation of thermal unfolding (DDG) was used to compare the wild-type and mutant NHases thermostability. The most improved …
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Structural and functional role of the extracellular loops and C-terminal domain of the voltage-gated sodium ion channel
… to some of these mutants form a complex salt bridge at the interface between the DII and DIII turret loops. Furthermore, adjacent aromatic residues could potentially form cation-π interactions with the complex salt bridge. This region was examined using site-directed mutagenesis, …
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Macromolecular recognition in the cytochrome P450(cam) enzyme system
… by looking for van der Waals complementarity and salt bridge formation between the cytochrome b$\sb5$ anionic binding surface and basic residues on the cytochrome P-450$\sb{\rm cam}$ surface. A good fit was found on the proximal surface of nearest approach to the cytochrome P-450$\sb{\rm cam}$ …
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An examination of interacting residues in the GABA-gated ion channel UNC-49 within the parasitic nematode Haemonchus contortus
… suggesting that they interact possibly through a salt-bridge. Disulfide trapping indicated that the two residues are in close enough proximity to directly interact. This analysis of key residues in a unique receptor could potentially be utilized for the future development of new anthelmintics to …
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Galvanic electrochemical cells: a study of the understanding and knowledge responses genera ted by students tested as individuals, in pairs and in groups
… electrode current flow, forms of energy, salt bridge, oxidation and reduction half reactions and industrial applications of electrochemical cells. The wording and content of the final full electrochemistry test was progressively improved through three pilot trials involving consecutive …
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Solid-state NMR studies of α-synuclein fibril structure
… notably the E46K mutant disrupting a stabilizing salt-bridge within the fibril core. Additionally, I show that non- native cysteine mutants of T33C, V48C, T59C, A85C, and Q99C further validate the structure. The results and studies presented here establish the first structure of α-syn fibrils and …
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CARATTERIZZAZIONE FARMACOLOGICA E FUNZIONALE DI NUOVI LIGANDI DEI RECETTORI COLINERGICI NICOTINICI NEURONALI CHE MODULANO IL RILASCIO DI DOPAMINA NELLA VIA MESOSTRIATALE, UNA VIA IMPORTANTE PER GLI EFFETTI COMPORTAMENTALI DELLA NICOTINA.
… recognition of PIA by α6β2* nAChRs depends on a salt bridge between the guanidine group of R1 and the highly negatively charged D166-D167 residues located on the β2 subunit. The RDP sequence was then added to the N-terminus of MII; the resulting hybrid peptide (RDP-MII) showed an increased …
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Computational Investigation of Protein-Membrane Systems Involved in Cellular Trafficking
… the cell membrane. We have identified a crucial salt-bridge that might play role in controlling the access of the sugar to the protein, and probed the molecular and energetic details of lactose translocation across LacY using steered molecular dynamics simulations."
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Mechanisms of chloride modulated activity in the C-domain of angiotensin-converting enzyme
… affinity in the chloride 2 pocket. The E403-K118 salt bridge in tACE was shown to stabilize the hinge-bending region and reduce chloride affinity by constraining the chloride 2 pocket, an interaction which is destabilized via substrate interactions within the S2 pocket which results in tighter …
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Solution and solid-state NMR studies of amphotericin B
… ion gradients and cause cell death. A ring of salt bridges and/or hydrogen bonds at the channel periphery are proposed to stabilize the channel assembly. These polar interactions are proposed to form between the C41 carboxylate and C3' amine of adjacent AmB molecules within the channel …
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Elucidation of the mycosamine dependent sterol binding and antifungal activities of amphotericin B
… ion channel formation via an intermolecular salt-bridge interaction, 2) anchoring AmB to phospholipid bilayers and 3) binding to sterols. However, an alternative hypothesis states that AmB does not directly bind sterols but rather that sterol-induced global membrane properties are the source …
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Refining computer-aided drug design routes for probing difficult protein targets and interfaces
… as well as highlighting the role of Arg119 in a salt-bridge network of interactions essential in substrate translocation. Collectively, this work illustrates the advantages of computational workflows in the drug discovery process and provides a framework that can be applied for additional GCPRs, …
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Structure and activity of human clusterin.
… by DEPC modification suggesting that a salt bridge between a clusterin imidazole and an acidic side chain of Abeta1-40, reminiscent of electrostatic interactions postulated to stabilize Abeta peptide monomers in polymeric fibrils, in addition to hydrophobic interactions may be important …
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Biochemical and Biophysical Characterization of Replicative Helicase Loading by <i>Vibrio cholerae</i> DciA, Crystal Structure of a Monoclonal Antibody Bound to a C-Terminal Fragment of EBNA-1 from Epstein Barr Virus
… by hydrophobic packing, hydrogen bonding, a salt bridge, and water molecules that bridge the two proteins. Bonding between 3D4 residues Asn35/Lys55 and LS9B residues Asp577/Asp581 is highlighted as a potential charge mimic of the 3D4-dsDNA interaction. A complete understanding of how 3D4 …
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The structure of the nitrilase from Rhodococcus Rhodochrous J1: homology modeling and three-dimensional reconstruction
… of the insertions and the possibility of salt bridge formation on the D surface suggest that spiral formation is common among microbial nitrilases. Furthermore, the presence of the C terminal domain in J1 nitrilase creates a steric hindrance that prevents spiral formation. When this is …
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The Mechanism of Bicarbonate Activation of Plastoquinone Reduction in Photosystem II of Photosynthesis
… action, in which one HCO$\sb{3}\sp{-}$ forms a salt bridge between the non-heme Fe$\sp{2+}$ in PS II and a histadine protein residue, another HCO$\sb{3}\sp{-}$ is involved in protonating a histidine near the Q$\sb{\rm B}$ site to stabilize Q$\sb{\rm B}\sp{-}$, and a low affinity pool of …
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