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Showing 1 to 20 of 29 for “"SYBR green"”.

  1. Accelerating antimalarial drug discovery through repositioning

    Matthews, H2026

    … goal, two, fast, simple, and reliable in vitro SYBR Green-based drug susceptibility assays were optimised. The first, the SYBR Green microplate method offered a medium throughput option that was used to screen two FDA-approved libraries (Z score = 0.68 +0.06), LOPAC and ENZO (~700 compounds). …

    salford Repository record

  2. Molecular typing of virulence genes in enterotoxigenic Bacillus cereus

    Gracias, Kiev S.2007

    … production of emetic toxins and enterotoxins. SYBR Green I is used as an intercalating dye and its florescence increases as a result of DNA amplification during real-time PCR. A second-derivative plot is obtained at the end of the PCR run, where amplicons are differentiated based on their DNA …

    ballstate-thes Repository record

  3. Nanoparticle sensors for the detection of DNA related to disease

    Barrett, LeeUniversity of Strathclyde2012

    … conjugates using a fluorescent DNA intercalator, SYBR green I, was developed. This study showed that SYBR green I can be used as a rapid method for determining the melting profiles of individual nanoparticle conjugates and the hybridisation efficiency of the immobilized oligonucleotides.

    strathclyde Repository record

  4. Combination of Dietary Tomato and Broccoli Powders Are Effective Growth Inhibitors of Dunning R3327-H Prostate Adenocarcinomas

    Adams, Kirstie Anne-MerreaUniversity of Illinois at Urbana-Champaign2015

    … time polymerase chain reactions (RT-PCR) and the SYBR Green assay, mRNA was quantified in the liver, prostate, and tumors. Finasteride significantly decreased expression of 5-alpha-reductase-II, Bcl-2, and IL-6 in tumors. IL-6 was reduced in high lycopene and broccoli, P=0.05, and TNF-alpha was …

    uiuc Repository record

  5. Development of a 16S rRNA gene sequence as a biomarker specific to the gastrointestinal tract and feces of swine based on suppressive subtractive hybridization

    Sayre, Autumn2019

    … marker were compared in a phylogenetic tree. The SYBR green qPCR assay did show amplification of sequences in cattle, human, and two non-target soil samples; however, apparent positive detection of the swine marker was rejected based on dissociation profiles. Therefore, the SYBR green qPCR assay …

    wvu Repository record

  6. Hybrid Cyclic/Linear Peptides in a Multi-Component Lipid Structure as a siRNA Delivery System

    Alasmari, Abdulaziz2022

    … to improve siRNA internalization into the cell. SYBR Green II dye exclusion assay showed a high binding affinity of the peptides with siRNA. The delivery of siRNA into the cells was further enhanced by incorporating hybrid peptides into a multi-component structure called Peptide/Lipid-Associated …

    chapman Repository record

  7. Artemisinin-Based Combination Therapy (ACTs) Drug Resistance Trends in <em>Plasmodium falciparum</em> Isolates in Southeast Asia

    Schilke, Jessica LDigital Commons @ University of South Florida2009

    … falciparum were cultured and then assayed using SYBR green as an indicator to obtain drug IC50s. The data suggested that many isolates are not demonstrating resistance to artemisinin. However, a select few are showing some resistant characteristics in the form of elevated IC 50s, especially to …

    usf Repository record

  8. DENVax live attenuated chimeric dengue vaccine

    Mulhern, Kaitlyn, author, Nyborg, Jennifer, advisor, Livengood, Jill, advisor, Stinchcomb, Dan, committee member, Blair, Carol, committee memberColorado State University. Libraries2010

    … using two fluorescence-based methodologies, SYBR Green and TaqMan. The SYBR Green method was used to test the virus samples and reproducible results were obtained. The TaqMan assay allows for multiplexing, which allows simultaneous detection of two PCR products; Vero cell DNA present in virus …

    colostate Repository record

  9. High resolution meltcurve PCR assay for detection of salmonella and escherichia coli O157:H7 in foods

    Liu, Yujiao, 1990-University of Missouri--Columbia2017

    … to yield a higher resolution plot compared with SYBR Green I PCR. HRM dyes completely saturate all amplicons without showing preferential bindings, making the results more clear and distinct. In this research, a multiplex real-time PCR targeting the invA, fimA and stn genes were developed to …

    missouri Repository record

  10. An evaluation of genetic markers for forensic identification of human body fluids

    Afolabi, OlatundeUniversity of Central Lancashire2017

    … limit of detection (LOD) were investigated using SYBR Green and Taqman probes. The results of the SYBR Green efficiency test experiment displayed five markers, UCE, TEF, ACTB, B2M, and RPS29 with 90-110% efficiency with a slope -3.33 ± 10%. Subsequently, Taqman probes were designed for the five …

    cent-lancashire Repository record

  11. Aumento diferencial da expressão de IL13 e CCL17 em mucosite: uma análise comparativa com saúde e peri-implantite

    Manso Filho, Marcelo Corrêa2026

    … A análise de RT-PCR foi realizada utilizando o SYBR green para cfRNA dos genes IL13, CCL17 e IL8. A análise dos dados clínicos como comorbidades sistêmicas, idade e sexo não demostraram diferença estatística para os três grupos, enquanto que o fenótipo peri-implantar classificado em fino ou …

    brazil-uff Repository record

  12. The Development of New Tools to Investigate Alphavirus Replication Kinetics

    Plaskon, Nicole ElyseVirginia Tech2009

    … compared to a double-stranded DNA-specific dye, SYBR Green. Using this information, we determined the replication kinetics of two different genotypes of o'nyong nyong virus (ONNV) and chikungunya virus (CHIKV) in infected mosquito cells. We found that (-) strand viral RNAs persisted in …

    vt Repository record

  13. Evaluation of the Role of Epstein-Barr virus and Cellular Gene Expression in Paediatric and Adult Transplant Recipients

    Auburn, HelenKing's College London2012

    … We used real-time two-step RT-PCR assays with SYBR green detection to analyse EBV gene expression (76 lytic, 12 latent [n = 88 genes]), following 12-O-tetradecanoyl-13-phorbol acetate/sodium butyrate (TPA/NaB) induction of Raji cells. We identified 22 highly induced EBV genes (>90-fold) at 24 …

    kings Repository record

  14. Transcriptional profile of c-kit positive cardiac stem-progenitor cells (c-kitpos eCSCs) isolated from the four chambers of the adult human heart

    Theologou, TLiverpool John Moores University2015

    … random hexamers. qRT-PCR was performed using SYBR Green on a MyIQ thermocycler Bio-Rad® of specific genes representative of the primary and secondary heart field, and the developmental program of their chamber of origin. Results: c-kitpos eCSCs isolated from 15 human samples (5LA, 1RV, 4LV, …

    liverpool-jm Repository record

  15. Gene expression of DNA repair proteins in colorectal cancer and medulloblastoma

    Odufuwa, K2026

    … were determined by quantitative PCR using the Sybr green method and analysed using the comparative Ct method and glyceraldehyde 3-phosphate dehydrogenase (Gapdh) as the standard. Of the matched samples investigated, 75% showed increased expression of one or more of the DNA repair genes …

    salford Repository record

  16. Investigating ethnopharmacology-based natural product leads for antimalarial drug discovery

    Idris-Usman, MS2026

    … assays including flow cytometry (FCM) and SYBR green microtitre assay (SG) were optimized to screen some aqueous plant extracts which were selected based on their ethnopharmacological usage. Giemsa light microscopy was used to validate the assays. Plasmodium berghei malaria animal model was …

    salford Repository record

  17. Detection of the calreticulin type 1 and type 2 mutations in a group of myeloproliferative neoplasm patients using molecular methods

    University of Pretoria2016

    … than the in-house PCR assay. The qPCR using SYBR Green as well as the qPCR using probes were unable to detect the type 1 and type 2 mutations and further troubleshooting was not possible due to budgetary constraints. Overall, 3/50 patients contained the type 1 mutation and 2/50 contained the …

    pretoria Repository record

  18. Immunomodulation of commercial line and Piau Brazilian Naturalized breed pigs in response to vaccination against Mycoplasma hyopneumoniae and Pasteurella multocida type D

    Sousa, Katiene Régia SilvaUniversidade Federal de Viçosa2011

    … usando o sistema de detecção de fluorescência SYBR Green, usando como controle endógeno os genes GAPDH e HPRT1 para PBMC e BALF, respectivamente. Para o experimento com Micoplasma hyopneumoniae como patógeno, a linhagem comercial teve maior expressão de mRNA para TLR2, TLR4, TLR6 e TLR10 e as …

    brazil-ufv Repository record

  19. Real Time PCR-Based Infectivity Assay and Characterization of Cell Surface Receptors for Turkey Hemorrhagic Enteritis Virus

    Mahsoub, Hassan Mostafa MohammedVirginia Tech2016

    … sensitivity, inaccuracy, and time consumption. A SYBR Green-based real time PCR assay for determining the genomic titer of THEV through the quantification of its hexon gene was developed. The assay was applied as a quality control for the titration of splenic vaccines and was found useful in …

    vt Repository record

  20. Investigating calcium channel blockers as antimalarials

    Rajab, M2026

    … available drugs was carried out using a SYBR Green (SG) plate reader assay. Both CalcuSyn-based combination studies and a chloroquine potentiation assay were carried out. This was succeeded by the synthesis of fendiline analogues, which were carried out via a two-step synthetic route …

    salford Repository record

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