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Showing 1 to 20 of 69 for “"SUMOylation"”.
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Developmentally regulated SUMOylation in the ciliate Tetrahymena thermophila
… to investigate developmentally regulated SUMOylation. We predicted a developmental increase in SUMOylation during late conjugation based on the extensive genome remodeling in the developing macronucleus (anlagen) of<em>Tetrahymena thermophila.</em> Immunoblotting of cell lysates from …
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Regulation of the oncogenic PIM1 kinase by SUMOylation
… site. However, the K169R mutant only abolished SUMOylation at one site, but the E171A mutant abolished SUMOylation at both sites. I also showed that members of the Protein inactivator of activated STATs (PIAS) family could act as E3 SUMO ligases for PIM1. SUMOylated PIM1 showed higher protein …
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Understanding The Role of Sumoylation In Regulating Lkb1 Function
… in governing LKB1 activity. However, the role of SUMOylation in regulating LKB1 function is unknown. SUMOylation is the reversible covalent modification of a SUMO (small ubiquitin-related modifier) protein to a specific lysine on the target protein. This process has been implicated in important …
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NEUROPROTECTIVE THERAPIES CENTRED ON POST-TRANSLATIONAL MODIFICATIONS BY SUMOYLATION
… MOdifier (SUMO) protein(s) via a process dubbed SUMOylation. The upregulation of this pathway has been shown to be capable of maintaining homeostasis in natural models (e.g. hibernation) and in preclinical models of brain ischemia. As such, identifying small molecules that upregulate the …
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Sumoylation of RNA Polymerase II Regulates Transcription in Saccharomyces cerevisiae
Sumoylation is a post-translational modification that is involved in a host of cellular processes, including important roles in transcription. While it was previously reported that Rpb1, the largest subunit of RNA polymerase II (RNAPII), is sumoylated following UV-stress, our lab has shown that …
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The Sumoylation of General Transcription Factors Regulates Transcription in Saccharomyces cerevisiae
Sumoylation is a post-translational modification which primarily targets nuclear substrates and can result in altered target protein activity, stability, conformation, and/or localization. Out of the six general transcription factors (GTFs), we have determined that transcription factor IID (TFIID) …
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THE ROLE OF PIAS PROTEINS IN THE SUMOYLATION OF 5-HT1A RECEPTORS
… to agonist. In addition, it was reported that SUMOylation of 5-HT1A Rs in the hypothalamic membrane increased by treatment with the 5-HT1A R agonist (+)-8-Hydroxy-2-dipropylaminotetralin ((+)8-OH-DPAT) and was further enhanced by combination of treatment of 17β-estradiol-3-benzoate (EB) and …
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The Role of Esc2 and DNA in Smc5/6-mediated Sgs1-Top3-Rmi1 Sumoylation
… process is regulated by Mms21-Smc5/6 mediated sumoylation of STR in the S and G2 phases. While STR is known to work in conjunction with Smc5/6 and SUMO, the exact details and mechanisms of how Mms21-Smc5/6 mediated STR sumoylation is regulated remain vastly unknown. This dissertation seeks to …
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Applications of Ion Mobility Mass Spectrometry - Screening for SUMOylation and Other Post-Translational Modifications
… Although much is known about the processes of SUMOylation and deSUMOylation, exactly how it mediates its various functions and 4 different human isoforms remains unclear. This is due in part to the extremely low abundance of modified proteins (about 2% of a given substrate will become …
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Interplay between collapsin response mediator protein 2 (CRMP2) phosphorylation and sumoylation modulates NaV1.7 trafficking
… trafficking and function. Expression of a CRMP2 SUMOylation incompetent mutant (CRMP2-K374A) in neuronal model CAD cells, which express predominantly Nav1.7 currents, led to a significant reduction in huwentoxin-IV-sensitive Nav1.7 currents. Increasing deSUMOylation with sentrin/SUMO-specific …
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Protein SUMOylation is a Sex-Specific Regulator of Fear Memory Formation in the Amygdala
SUMOylation is a type of post-translational protein modification similar to ubiquitination and it involves the covalent attachment of a small ubiquitin-like modifier (SUMO) protein to the lysine residue of a target substrate. While there is strong evidence for the role of protein ubiquitination in …
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Sumo-Specific Protease 1 Controls Lymphoid Development Through Regulation of Sumoylation/Acetylation Switch In Stat5
<p>SUMOylation has emerged as an important regulatory mechanism for protein function. SUMO-specific proteases (SENPs) are essential for removing SUMO from conjugated proteins in many different systems, but the physiological functions of SENPs are poorly understood. STAT5 (Signal Transducer and …
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Regulation of INTS-6 by post-translational modifications: exploring phosphorylation, SUMOylation and their biological relevance in Caenorhabditis elegans
… damage repair by homologous recombination, and SUMOylation, which could influence other cellular processes, such as the subcellular localization of the protein or the formation of protein complexes. Finally, this work aims to highlight the relevance of both basic research, which is essential for …
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Examining the Role of DAX-1 in Regulation of Cell Proliferation in Human Breast Cells
… one type of posttranslational modification, SUMOylation. SUMOylation involves the addition of the small polypeptide conjugate SUMO (<strong>S</strong>mall <strong>U</strong>biquitin-like <strong>Mo</strong>difier) to proteins. SUMOylation can have a variety of effects on target proteins …
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Regulation of mGluR7 trafficking by SUMOylatio in neurons
Protein SUMOylation is a post-translationalmodification by which SmallUbiquitin-like MOdifier (SUMO) proteins are covalently linked to the lysine residues of target proteins via an enzymatic cascade. It has been reported that SUMOylation of synaptic proteins plays important regulatory roles in …
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The Role Of Ubc9 In Drug Resistance And Its Expression Regulation In Cancer Cells
As a posttranslational modification, the sumoylation pathway plays a key role in a wide variety of cellular events such as cell proliferation, differentiation, stress response, DNA repair and apoptosis. Given the important role of protein sumoylation, it is not surprising that alternation of …
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Ehrlichia chaffeensis Type 1 Secreted Effector TRP47 is a Novel SUMOylated Nucleomodulin
… TRP47 was identified as a target for host cell SUMOylation and as a novel nucleomodulin with DNA-binding activity and host nuclear localization. Using a microfluidic chip peptide array, a SUMOylation site in the TRP47 N-terminal domain was identified. A SUMO-2 modification was further confirmed …
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Coordinated of Post-Translational Modifications of Yeast Transcriptional Activator Gcn4
… However, it is not clear how promoter-associated sumoylation of Gcn4 is coordinated with its phosphorylation and ubiquitination as part of a regulatory cascade of PTMs. Our results indicate that Gcn4 sumoylation occurs independently of its phosphorylation or ubiquitination; however, some degree of …
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Analyzing Sumo-2/3 Modification In Regulation Of Breast Cancer Progression And Mitotic Chromosome Segregation
<p>Roles of SUMOylation in cancer metastasis and mitotic progression has been elucidated in this thesis. This vital and dynamic post-translational modification has been known to be dysregulated in several kinds of cancer. Global SUMO-2/3 conjugation is upregulated in a mouse metastatic breast …
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Effects of altered expression of the sumo conjugating enzyme, UBC9 on mitosis, meiosis and conjugation in Tetrahymena thermophila
<p>SUMOylation is a critical posttranslational modification in eukaryotic species. Ubc9p is the E2-conjugating enzyme for SUMOylation and consequently it influences multiple cellular pathways. Nuclear proteins are common targets of SUMOylation and regulate nuclear events such as transcription, DNA …
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