Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 102 for “"SUMO"”.
-
Global identification of SUMO modification sites
… modification by Small Ubiquitin-like modifiers (SUMOs) affects the behaviour of proteins involved in diverse cellular processes and is indispensable for cell survival. Proteomic studies have identified hundreds of putative targets of SUMO conjugation, but understanding the substrate-specific …
-
Npsd4: A New Player In Sumo-Dependent Dna Repair
… of post-translational modifications, including SUMOylation. Through proteomic analysis of proteins involved in SUMOylation following DNA damage, our lab identified an uncharacterized protein that we named New Player in SUMO-dependent DNA damage repair 4 (NPSD4). Through an additional proteomic …
-
Characterizing the Role of SUMO in Transcriptional Repression in S. cerevisiae
Small ubiquitin-like modifier, SUMO, is a member of the ubiquitin-like protein family. Similar to ubiquitin, SUMO is conjugated to target proteins as a post-translational modification via an E1, E2, and E3 enzymatic cascade, in a process known as SUMOylation. SUMOylation leads to altered target …
-
Amyloid-beta signaling in physiology and pathology: from astrocytes to SUMO
… with the small ubiquitin-like modifier (SUMO) is required for normal synaptic and cognitive function but can be impaired by Abeta oligomers. I discovered that SUMOylation was significantly reduced in brain tissue from AD patients and a transgenic mouse model of AD. While neuronal …
-
Sumo and Ubiquitin Ligases Regulate Hepatocyte Nuclear Factor - 1 Beta Transcriptional Activity
… identify proteins that interact with HNF-1β The SUMO E2 ligase, Ubc9, SUMO E3 ligases, PIAS1 and PIASgamma , and the ubiquitin E3 ligase, Arkadia, were isolated as putative interacting proteins. SUMOylation of transcription factors affects their subcellular localization, intracellular transport, …
-
The identification and characterisation of proteins interacting with SUMO-ubiquitin hybrid chains
… ubiquitin or the small ubiquitin-like modifier, SUMO. In recent years, points of convergence between the once thought distinct and independent SUMO- and ubiquitin-conjugation pathways have been identified. The ubiquitin E3 ligase RNF4 catalyses the addition of ubiquitin modifications to …
-
RAD6-abhängige DNA-Reparatur wird durch Ubiquitin- und SUMO-Modifikation von PCNA reguliert
… ähnlichen Proteine,wie beispielsweise SUMO,sind ebenfalls nicht direkt am Proteinabbau beteiligt.Die Konsequenz der Modifikation,und das Zusammenspiel Ubiquitin-ähnlicher-Systeme mit dem Ubiquitin-System sind bisher allerdings wenig verstanden. PCNA (Proliferating Cell Nuclear …
-
Analyzing Sumo-2/3 Modification In Regulation Of Breast Cancer Progression And Mitotic Chromosome Segregation
<p>Roles of SUMOylation in cancer metastasis and mitotic progression has been elucidated in this thesis. This vital and dynamic post-translational modification has been known to be dysregulated in several kinds of cancer. Global SUMO-2/3 conjugation is upregulated in a mouse metastatic breast …
-
Sumo-Specific Protease 1 Controls Lymphoid Development Through Regulation of Sumoylation/Acetylation Switch In Stat5
<p>SUMOylation has emerged as an important regulatory mechanism for protein function. SUMO-specific proteases (SENPs) are essential for removing SUMO from conjugated proteins in many different systems, but the physiological functions of SENPs are poorly understood. STAT5 (Signal Transducer and …
-
De novo synthesis of high purity CD3 epsilon peptides utilizing SUMO expression system in bacteria
… peptide variants is required. By utilizing the SUMO (Small Ubiquitin-related Modifier) system from yeast, CD3ε1-27 amino acid and a truncated version 2-27 amino acid peptide are expressed in E. coli cells with an SMT3 (Mitotic Fidelity Gene 3) tag. Subsequently, SMT3 tag is removed with SUMO …
-
Effects of altered expression of the sumo conjugating enzyme, UBC9 on mitosis, meiosis and conjugation in Tetrahymena thermophila
<p>SUMOylation is a critical posttranslational modification in eukaryotic species. Ubc9p is the E2-conjugating enzyme for SUMOylation and consequently it influences multiple cellular pathways. Nuclear proteins are common targets of SUMOylation and regulate nuclear events such as transcription, DNA …
-
Characterisation of Small Ubiquitin-like Modifier (SUMO) protein complex expression in human cancer cells to identify novel therapeutic targets
SUMOylation is a post-translational modification where a number of small ubiquitin-like modifier proteins (SUMO) are conjugated to lysine residues of the target proteins and contribute to various cellular functions essential for cancer cell survival and proliferation. The unique behaviours, …
-
Regulation of mGluR7 trafficking by SUMOylatio in neurons
Protein SUMOylation is a post-translationalmodification by which SmallUbiquitin-like MOdifier (SUMO) proteins are covalently linked to the lysine residues of target proteins via an enzymatic cascade. It has been reported that SUMOylation of synaptic proteins plays important regulatory roles in …
-
SUMOylierung von NFYA
… mit Ubiquitin-ähnlichen Proteinen der SUMO-Familie ist ein wichtiger Mechanismus, um Proteine in ihrer Funktion, Aktivität oder Lokalisation zu beeinflussen. Die kovalente Anheftung von SUMO erfordert das Zusammenspiel einer Enzymkaskade von einem E1, einem E2 Enzym und in den meisten …
-
Biochemical Characterization of the Activity and Specificity of ULP1 Family Members
The reversible posttranslational modification, SUMO, modulates the activity of a diverse set of target proteins. Conjugation machinery charges the processed-SUMO so that it can be linked via an isopeptide bond to a target protein. The removal of SUMO moieties from conjugated proteins by …
-
Novel Insights into Ubiquitin-like Protein E1-E2 Interactions
… proteins (UBLs) such as ubiquitin, Sumo and NEDD8 regulate a vast array of processes in the cell. Transfer of UBLs to their target generally occurs by a series of molecular handoffs down an E1‑E2‑E3 cascade. We are interested in understanding how E1‑E2 pairs interact and mediate UBL …
-
Characterizing the RanGAP1-RanBP2 complex in mitosis
… zum anderen besitzt RanBP2 zusammen mit Ubc9 Sumo-konjugierende Aktivität. Gemeinsam sind diese Proteine essentielle Regulatoren des nukleo-zytoplasmatischen Transportes in Interphasezellen, und spielen des Weiteren eine wichtige, bislang jedoch kaum verstandene Rolle für die Funktion der …
-
Rekombinantinio TDP-43 baltymo sintezės ir gryninimo optimizavimas /
… the synthesis and purification of recombinant SUMO-TDP-43 protein in E. coli cells. The targeting protein is important in the context of neurodegenerative diseases, as accumulations of amyloid TDP-43 aggregates cause cell damage. The preparations carried out in this work included the synthesis …
-
Developmentally regulated SUMOylation in the ciliate Tetrahymena thermophila
<p>The Small Ubiquitin-like MOdifier (SUMO) protein regulates numerous nuclear events such as transcription, mitosis and meiosis and DNA repair. These processes are critical to the programmed nuclear events of conjugation in ciliates and provide the potential to investigate developmentally …
Page 1 of 6