Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"STED microscopy"”.
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STED microscopy in the visible range
Light microscopy is a key scientific instrument in the life sciences. However, the resolution of far-field light microscopy is limited by diffraction. Exploiting a saturated depletion of the molecular excited state by stimulated emission, stimulated emission depletion (STED) breaks the resolution …
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Dual-Colour STED-microscopy on the Nanoscale
Stimulated emission depletion (STED) microscopy was the first concept for breaking Abbe's diffraction barrier in optical far-field microscopy verified in biological applications. However, the theoretically infinite resolution was limited due to photobleaching of the fluorescent species. In this …
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A general method for three color STED microscopy with one depletion laser : application to primary neuronal culture
… accessible platform for super-resolution microscopy (SRM) of synaptic nanostructure.
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Stimulated Emission Depletion (STED) Microscopy and Pacific Orange Dye Optimization For H9C2 Cox-1 Imaging Via Indirect Immunocytochemistry
<p>The fundamental barrier of traditional microscopy has always been the Abbe limit. Diffraction has served to limit image production and microscopic investigation on the sub-cellular level, greatly hindering microbiology and other forms of study. Stimulated Emission Depletion microscopy is one of …
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Adaptive optics stimulated emission depletion microscope for thick sample imaging
Over the past few decades, fluorescence microscopy has proven to become the most widely used imaging technique in the field of life sciences. Unfortunately, all classical optical microscopy techniques have one thing in common: their resolution is limited by the diffraction. Thankfully, due to the …
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Development of the multi-scale dynamic modeling techniques for multi-modal brain recordings
… (~200 nm), we need an in-vivo super-resolution microscopy to achieve our goal. As a super-resolution imaging method, stimulated emission depletion (STED) microscopy has unraveled fine intracellular structures and provided insights into nanoscale organizations in cells. Although image resolution …
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Development and application of correlative STED and AFM to investigate neuronal cells
… migration a technique combining atomic force microscopy (AFM) with stimulated emission depletion (STED) microscopy was developed. First a custom STED microscope was developed. To facilitate the design of this system the theoretical performance of a range of STED techniques (cw-STED, time-gated …
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Diabetes and the Female Heart
… super-resolution Stimulated Emission Depletion (STED) microscopy and automated image analysis pipelines to quantify key ECC proteins organisation: transverse tubules (T tubule), ryanodine receptor type 2 (RyR2) clusters, and filamentous actin (F-actin). Results showed that while diabetic rats …
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Investigating New Guaiazulenes and Diketopyrropyrroles for Photonic Applications
… DPPs have motivated their study for fluorescence microscopy applications, concluding that the prepared DPP's are potentially suitable chromophores for high resolution stimulated emission depletion (STED) microscopy.</p>
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Untersuchung sub-mitochondrialer Proteinverteilungen in unbehandelten und apoptotischen humanen Zellen mittels STED-Mikroskopie
… lichtmikroskopischer Techniken wie der STED-Mikroskopie erlaubt es seit kurzer Zeit sub-mitochondriale Proteinverteilungen zu untersuchen, wobei bislang ausschließlich qualitative Studien durchgeführt wurden. In dieser Arbeit wurde die STED-Mikroskopie mit mathematischen Algorithmen …
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Synaptic vesicle recycling <i>in Vivo</i>
… zurück halten. Durch Antikörperfärbung und STED (stimulated emission depletion) Mikroskopie konnte gezeigt werden, dass Vesikelcluster (die hauptsächlich nicht-aktive Vesikel enthalten) die entsprechenden Proteine anreichern, was auch durch Immunoblotting bestätigt werden konnte. In Einklang …