Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 26 for “"STED"”.
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STED-4Pi Microscopy
Saturated stimulated emission depletion (STED) has been shown to break Abbe's resolution limit in fluorescence microscopy. This technique suppresses fluorescence at the focal rim by use of a depleting laser pulse. In this thesis, theoretical and experimental examinations show that the combination …
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STED microscopy in the visible range
… emission, stimulated emission depletion (STED) breaks the resolution barrier in the important subfield of fluorescence microscopy. To this end, STED microscopy utilizes a doughnut-shaped beam featuring a central zero which is capable of quenching the fluorescence solely in the focal …
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Dual-Colour STED-microscopy on the Nanoscale
Stimulated emission depletion (STED) microscopy was the first concept for breaking Abbe's diffraction barrier in optical far-field microscopy verified in biological applications. However, the theoretically infinite resolution was limited due to photobleaching of the fluorescent species. In this …
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Structural characterization of primary cilia using accelerated piezoelectrically driven STED nanoscopy
… system using the stimulated emission depletion (STED) technique with novel accelerated piezoelectric control (apSTED). This improved STED system achieved a reduction in photobleaching rates from ~80% to ~10% while maintaining superresolution, ~50 nm at the focal plane for biological samples. …
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Development and application of correlative STED and AFM to investigate neuronal cells
… (AFM) with stimulated emission depletion (STED) microscopy was developed. First a custom STED microscope was developed. To facilitate the design of this system the theoretical performance of a range of STED techniques (cw-STED, time-gated STED, pulsed STED and RESOLFT) were compared, …
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Untersuchung sub-mitochondrialer Proteinverteilungen in unbehandelten und apoptotischen humanen Zellen mittels STED-Mikroskopie
… lichtmikroskopischer Techniken wie der STED-Mikroskopie erlaubt es seit kurzer Zeit sub-mitochondriale Proteinverteilungen zu untersuchen, wobei bislang ausschließlich qualitative Studien durchgeführt wurden. In dieser Arbeit wurde die STED-Mikroskopie mit mathematischen Algorithmen …
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A general method for three color STED microscopy with one depletion laser : application to primary neuronal culture
Recent advances in the field of optical nanoscopy have equipped molecular neuroscientists with the means to investigate sub-synaptic phenomena with unprecedented spatial resolution. Indeed, early studies employing these methodologies have revealed an extraordinary level of nanoscale spatiotemporal …
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Stimulated Emission Depletion (STED) Microscopy and Pacific Orange Dye Optimization For H9C2 Cox-1 Imaging Via Indirect Immunocytochemistry
… utilizing multiple competing sources of light, STED has produced high-resolution, nanoscale images of both biological and non-biological samples, greatly adding to the wealth of knowledge in multiple disciplines. Despite these contributions, several obstacles remain for STED technology. These …
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Fluorescence Depletion by Stimulated Emission in Single-Molecule Spectroscopy
… of the stimulated emission depletion (STED) technique to the field of single-molecule fluorescence spectroscopy. It is demonstrated that fluorescence quenching induced by STED is reversible and can be repeated a large number of cycles on a single molecule. Being ideal point-like probes, …
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Adaptive optics stimulated emission depletion microscope for thick sample imaging
… techniques, Stimulated Emission Depletion (STED) microscopy has been particularly interesting, as it is a purely optical technique which does not require post image processing. STED microscopy has proven to resolve structures down to the molecular resolution. However, super-resolution …
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Idéen om Island - Et sted mellem utopisk drøm og virkelighed. En imagologisk anskuelse af formidlingen af en nation gennem det kunstneriske udtryk.
Forestillingen om Island har rødder helt tilbage til landets første bosættelse, og hvis historiske fortid og såvel sande beretninger som fejlagtige antagelser, samt diverse interesser og traditioner har haft indflydelse på, hvorledes Island opfattes i vor samtid. Denne opgave beskæftiger sig med …
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Development of the multi-scale dynamic modeling techniques for multi-modal brain recordings
… imaging method, stimulated emission depletion (STED) microscopy has unraveled fine intracellular structures and provided insights into nanoscale organizations in cells. Although image resolution can be further enhanced by continuously increasing the STED-beam power, the resulting photodamage and …
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Nanolithography and nanoscopy methods for the study of biological samples in confined spaces
… diseases in vitro, a custom-built pulsed-STED system was equipped with a second excitation beam path to allow for two-colour STED imaging using the same IR depletion wavelength. The system is then used on three different biological imaging applications. Single-color STED imaging is used to …
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Struktur und Funktion der afferenten Synapse innerer Haarzellen der Cochlea
… die Synapsen mittels höchstauflösender 4Pi- und STED-Mikroskopie untersucht. Dabei zeigte sich, das das präsynaptischen Band (Ribbon) einheitlich an verschiedenen tonotopen Orten eine Größe von etwa 360 nm besitzt. Auch das präsynaptische Cluster von Cav1.3-L-Typ-Calciumkanälen zeigte keine …
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The Roles of a Conserved MAP Kinase Pathway in the Regulation of Development and Secondary Metabolism in Aspergillus Species
… and Fus3 respectively) and the adaptor protein SteD (ortholog of yeast Ste50). However, there are no orthologs of the Ste5 scaffold in filamentous fungal genomes, suggesting a unique mechanism of regulation exists for the pheromone module pathway in species like A. nidulans. This led to the …
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Optimierung von photophysikalischen Eigenschaften organischer Farbstoffe zur Auflösungserhöhung
… Reduktion des Farbstoffmoleküls. Mit Hilfe der STED Mikroskopie kann gezeigt werden, dass über die Optimierung der photophysikalischen Eigenschaften die Beugungsgrenze gebrochen werden kann und Auflösungen im Bereich von 25 nm an einzelnen Farbstoffmolekülen möglich sind. Im letzen Kapitel liegt …
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Charakterisierung des mitochondrialen Teilungsproteins Dnm1p mittels quantitativer hochauflösender Lichtmikroskopie
… neue hochauflösende Lichtmikroskope (4Pi und STED) zur genauen Lokalisation und Größenbestimmung eingesetzt. Die Ergebnisse zeigen, dass im Wildtyp und in Mdv1p-Deletionsstämmen die Mehrheit der Cluster mit den Mitochondrien assoziiert ist, während in Fis1p- und Caf4p-Deletionszellen die …
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Diabetes and the Female Heart
… super-resolution Stimulated Emission Depletion (STED) microscopy and automated image analysis pipelines to quantify key ECC proteins organisation: transverse tubules (T tubule), ryanodine receptor type 2 (RyR2) clusters, and filamentous actin (F-actin). Results showed that while diabetic rats …
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Expansion microscopy : scalable and multiplexed nanoscale imaging
… beyond the diffraction limit (e.g., STORM/PALM, STED, SIM), while yielding similar performance. Expanded tissues are also optically clear, allowing for unprecedented super-resolution imaging in thick tissues and facile reagent diffusion into the sample. We have since developed a variant of ExM, …
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Grenzen und Möglichkeiten des Förster Resonanz-Energietransfer : einzelmolekülfluoreszenzspektroskopische Untersuchung an starren Systemen
… ermöglichen. Dazu zählen sowohl die STED-Mikroskopie (engl. stimulated emission depletion) als auch STORM (engl. stochastic optical reconstruction microscopy). Im Gegensatz zu diesen Techniken zählt der Förster Resonanz-Energietransfer (FRET) nicht zu den bildgebenden Verfahren. FRET …
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