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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"SOS response"”.
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Strategies to potentiate bactericidal antimicrobial activity based on the suppression of bacterial stress response systems
… to combat resistance and heteroresistance. The SOS response is a coordinated cellular response to genotoxic damage that may contribute to the evolution of antimicrobial resistance, since genes related to DNA repair and recombination processes are transcribed during the SOS response. Certain …
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Regulation of E. coli SOS mutagenesis by dimeric intrinsically disordered umuD gene products
… translesion DNA synthesis (TLS) during the SOS response to DNA damage. Homodimeric UmuD2 is upregulated early during the SOS response, and a slow post-translational autocleavage process removes the N-terminal 24 amino acids of each UmuD monomer. The remaining C-terminal fragment, UmuD'2, …
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Cellular responses to external threats probed by super-resolution microscopy
… infection in mammalian cells triggers the immune response, a cascade of antiviral signaling proteins. For double stranded RNA (dsRNA) virus, the innate immune response starts from retinoic acid-inducible gene-I (RIG-I) protein which is a dsRNA sensor. For certain types of viruses, RIG-I localizes …
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Elucidation of mechanisms of antibiotic subversion in mycobacteria
… of antitubercular agents. The DNA damage response: the SOS response was hypothesized to play a role in antibiotic- mediated cellular death, and that disabling the mycobacterial SOS response, by generating non-cleavable LexA mutants (lexA Ind-), could be used as a tool to validate …
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Characterization of Vancomycin Resistance in Staphylococcus Aureus
… increased strain survival in vancomycin. The SOS response was examined as the possible link between conversion to homotypic oxacillin resistance and increasing vancomycin resistance due to a thickened cell wall. The current study, however, detected no induction of the SOS response during …
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Collateral sensitivity and the evolution of drug resistance in Escherichia coli
… in mutation is due to antibiotic related SOS response, and the second is that multi-drug-resistance was selected for in both cases. This highlights the need for evolutionary considerations in designing new drug therapies, as apparent patterns of collateral sensitivity may not be a …
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Studies of DNA repair and mutagenesis in Escherichia coli and Bacillus subtilis
… systems. In bacteria, there exists an inducible response to DNA damage termed the SOS response, in which damage induces the expression of greater than forty genes involved in repair. Mismatch repair (MMR) is a system by which the cell is able to avoid mutations by correcting DNA replication …
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Molecular mechanisms of DNA repair in Mycobacterium tuberculosis
… investigate the contribution of the DNA damage (SOS) response in adaptive mutagenesis, and focused on two specific components: the role of the specialist translesion synthesis DNA polymerase, DnaE2, in mutagenesis under stress and, secondly, the function of the mycobacterial homologue of a …
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Generation of SOS inhibitors as co-drugs to potentiate the activity of bactericidal antibiotics and to block the emergence of antibiotic resistance
… as adaptive mutation, which is activated by the SOS DNA repair and mutagenesis pathways. Accumulation of single-stranded DNA (ss-DNA) is the signal that induces the SOS response by promoting the formation of the RecA filament, which in turn activates the auto-cleavage activity of LexA and allows …
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Regulation of the mobile genetic element ICEBs1 by a conserved repressor and anti-repressor
… found in the Bacillus subtilis chromosome. The SOS response and the RapI-Phrl sensory system activate ICEBsl gene expression, excision, and transfer by inactivating the ICEBsl repressor protein ImmR. Although ImmR is similar to many characterized phage repressors, we found that, unlike these …
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On the Repair of DNA Breaks and the Specificity of the EcoRI Restriction Enzyme
… methylases are expressed in E. coli, the SOS DNA repair response is induced. This DNA damage is inflicted by E. coli restriction enzymes that cleave adenine (Mrr) or cytosine (McrB) methylated DNA. The genes encoding four of the five known E. coli restriction systems lie clustered …
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Use of a direct, positive selection strategy to generate improved prodrug-activating enzymes for cancer gene therapy
… which utilises bacteriophage lambda and the SOS response, was used to select NTR variants with increase sensitivity for CB1954 from each library. NTR mutants with greatest sensitivity were analysed by bacterial IC50 assays. Despite considerable work to generate and optimise the large library, …
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Consequences of a mobile genetic element integrated at secondary locations
… and/or viability, as well as induction of the SOS response. These effects are likely due to DNA damage resulting from plasmid-like, rolling-circle replication of the excision-defective ICEBs1 in the chromosome. Consistent with this model, deletion of ICEBs] replication genes (nicK and helP) …
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Subcellular localization and visualization of RecA and ImuAʹ in mycobacteria
… in Mtb. RecA is a key regulatory protein of the SOS response and ImuAʹ is a component of the mycobacterial mutasome, effecting DNA damage tolerance and mutagenesis. In this study, a comprehensive panel of M. smegmatis (Msm) RecA and ImuAʹ reporter strains was generated to explore the dynamics of …
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Quinolone mechanism of action: sensitivity, mutagenesis and tolerance
… One key effort is characterizing bacterial responses to antibiotic drug treatment to generate a more comprehensive understanding of the factors that contribute to cell death and to elucidate potential targets for new therapies. Quinolones are an important class of antibiotics that target DNA …
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The role of recombinational repair in the mechanism of killing during thymine starvation in Escherichia coli
… to accumulate chromosomal breaks and induce the SOS response, indicating chromosomal damages, but it is unclear if the chromosomal damage is the cause of lethality. The goal of this work is to elucidate the mechanism of thymineless death, by investigating if the killing is caused by formation of …
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Interplay between an integrative and conjugative element and its bacterial host
… on ICEBs1 inhibit the host DNA damage repair response by preventing RecA filament formation. This activity confers a fitness advantage to the host in that it dampens the SOS response and protects against phage activation while also stabilizing ICEBs1 DNA. I also present preliminary evidence …
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Differential Lipidomic and Proteomic Responses Induced by Sub-lethal Drug Challenge in Susceptible and Drug Resistant Mycobacterium smegmatis
… mycobacterial growth slows and stress response networks, including the SOS response, are temporarily activated. For both susceptible and resistant bacteria, cell wall remodelling begins early through dysregulation of cell wall and lipid synthesis enzymes — such as MtrAB, Mur proteins …
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Bioinformatic identification and characterization of cytokinetic regulators in Mtb
… placement, and rv2216 as encoding a potential SOS-associated cell division inhibitor, SulA. Bioinformatic-based assessments of orthology revealed a differential lineage than anticipated for the proteins encoded by both open reading frames (ORFs). We describe these two novel regulators in Mtb …
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Electrochemical Control of Bacterial Persister Cells
… resistance, pyocin-related functions, and SOS response. Comparative transmission electron microscopy (TEM) studies of the stationary phase <em>P. aeruginosa</em> PAO1 cells confirmed that DC treatments resulted in the compartmentalization of intracellular contents, release of outer membrane …
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